[ome-users] Bio-formats - Bug while parsing Micro-Manager metadata
Stephane Dallongeville
stephane.dallongeville at pasteur.fr
Thu Apr 6 16:45:09 BST 2017
Dear Bio-formats team,
We encounter a problem (verified on last Bio-Formats version) when we
try to load a Micro-Manager generated dataset with the metadata text file.
I attached one of the image of the dataset (don't need to provide the
whole dataset) and the problematic metadata.txt file.
Here's the error log we obtain when we try to open the image with the
metadata.txt file in the same folder :
java.lang.StringIndexOutOfBoundsException: String index out of range: -2
at java.lang.String.substring(String.java:1911)
at
loci.formats.in.MicromanagerReader.parsePosition(MicromanagerReader.java:808)
at
loci.formats.in.MicromanagerReader.parsePosition(MicromanagerReader.java:429)
at
loci.formats.in.MicromanagerReader.initFile(MicromanagerReader.java:297)
at loci.formats.FormatReader.setId(FormatReader.java:1397)
at
plugins.kernel.importer.LociImporterPlugin.open(LociImporterPlugin.java:496)
at icy.file.Loader.internalLoadSingle(Loader.java:3101)
at icy.file.Loader.loadSequences(Loader.java:2707)
at icy.file.Loader$7.run(Loader.java:2371)
at
java.util.concurrent.Executors$RunnableAdapter.call(Executors.java:471)
at java.util.concurrent.FutureTask.run(FutureTask.java:262)
at
java.util.concurrent.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1145)
at
java.util.concurrent.ThreadPoolExecutor$Worker.run(ThreadPoolExecutor.java:615)
at java.lang.Thread.run(Thread.java:745)
If we remove the metadata.txt file the problem disappears.
Hope it helps to resolve the issue :)
Best,
--
Stephane Dallongeville
Unité d'Analyse d'Images Biologiques
CNRS UMR 3691
Institut Pasteur
25 rue du Dr. Roux
75724 Paris cedex 15 (FRANCE)
Tel: +33 (0)1 45 68 87 01
Fax: +33 (0)1 40 61 33 30
http://www.bioimageanalysis.org/
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{
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:39 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 0,
"TimeFirst": false
},
"FrameKey-0-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 0,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000000_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 0,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 0,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "8056c9ed-0786-48cb-8954-c056fbc5997b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Focusing",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:04:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 417,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:39 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 0,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 0,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-1-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 1,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000001_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 1,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "2feadae1-201a-47b6-bbe9-015341294b7e",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:08:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 240243,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 1,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-2-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 2,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000002_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 2,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "20347239-2b58-4c83-8649-9b9402428a4c",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:12:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 480245,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 2,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-3-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 3,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000003_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 3,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "f8aec704-40dc-492f-9949-42814280783c",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:16:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 720240,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 3,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-4-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 4,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000004_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 4,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "fc694ff4-25a4-4d17-af55-174be2546784",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:20:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 960237,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 4,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-5-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 5,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000005_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 5,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "59063f9b-52f6-4c14-8e7f-691edb6af415",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:24:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 1200236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 5,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-6-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 6,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000006_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 6,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "80894516-3652-4d4b-bbc0-1cea1d054a45",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:28:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 1440236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 6,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-7-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 7,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000007_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 7,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "3a5c0497-a3c4-4290-afcd-179b88c0f6ee",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:32:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 1680233,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 7,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-8-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 8,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000008_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 8,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "b1a5eb1c-d185-4aaa-9d58-da3acd3c9e77",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:36:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 1920247,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 8,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-9-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 9,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000009_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 9,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "15959b46-3bbf-44d6-91a5-f285937c61be",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:40:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 2160236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 9,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-10-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 10,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000010_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 10,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "94e26c50-d0e5-4e1b-9bbe-0e768fac1b9c",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:44:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 2400242,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 10,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-11-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 11,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000011_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 11,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "627ec3ad-9eb8-4df3-bde6-efcfd85e6710",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:48:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 2640244,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 11,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-12-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 12,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000012_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 12,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "36e4e53f-dcad-452f-9aa5-e3f141c0c509",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:52:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 2880242,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 12,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-13-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 13,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000013_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 13,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "f935df7c-782c-482b-8ea4-3f92db4c1f38",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 16:56:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 3120246,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 13,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-14-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 14,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000014_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 14,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "ea20be9f-4ad2-44cf-893f-0bc3911b2026",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:00:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 3360249,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 14,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-15-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 15,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000015_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 15,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "dc9945f2-4438-4cc3-87f7-09a5b95b8a93",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:04:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 3600233,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 15,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-16-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 16,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000016_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 16,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "5654808b-1169-435a-a698-1f10c134bf91",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:08:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 3840299,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 16,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-17-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 17,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000017_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 17,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "e0505fd4-1b1a-4510-988f-7d6a03b38c96",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:12:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 4080236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 17,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-18-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 18,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000018_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 18,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "ac176ecf-cb17-4155-8033-c13d20c87f07",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:16:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 4320237,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 18,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-19-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 19,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000019_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 19,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "ae9c20aa-4bcc-44ff-afef-49e9e922e1e2",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:20:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 4560237,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 19,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-20-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 20,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000020_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 20,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "b1743a61-d25c-44d7-8c32-7c47475d6af6",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:24:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 4800242,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 20,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-21-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 21,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000021_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 21,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "2942c7bd-3721-4824-ab51-31f238ffca7c",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:28:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 5040231,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 21,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-22-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 22,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000022_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 22,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "24d71d35-5f36-4266-9a2a-dde681ecc16e",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:32:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 5280234,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 22,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-23-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 23,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000023_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 23,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "eaaa2dff-f4af-4de9-9328-ce969d048330",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:36:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 5520234,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 23,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-24-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 24,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000024_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 24,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6fdb5faf-961c-4a67-99df-1c44e275fd2c",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:40:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 5760239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 24,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-25-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 25,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000025_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 25,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "9abdc58d-a16b-4ca1-aee5-1d28beb33f5b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:44:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 6000235,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 25,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-26-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 26,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000026_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 26,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "637d9f16-aca1-4d73-8f67-b97f1da3d838",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:48:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 6240243,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 26,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-27-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 27,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000027_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 27,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d99ae705-2f47-4804-8468-c3f6b2e7e988",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:52:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 6480235,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 27,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-28-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 28,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000028_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 28,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "b0618d4a-fd87-444d-baa0-d2cdb0fb7c01",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 17:56:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 6720242,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 28,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-29-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 29,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000029_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 29,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "56c802c6-448d-4adb-8150-78e81c21f091",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:00:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 6960235,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 29,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-30-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 30,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000030_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 30,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "ba38c365-6536-4a09-91f1-123a64b0839d",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:04:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 7200234,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 30,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-31-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 31,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000031_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 31,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6ef62f6a-9faa-4eb2-909d-6a200c44ce18",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:08:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 7440248,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 31,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-32-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 32,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000032_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 32,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "9717ee9d-2fce-475b-8fc0-fa6d40dd8019",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:12:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 7680227,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 32,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-33-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 33,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000033_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 33,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "1de89083-b530-4fc7-bc64-abfb6a9542bd",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:16:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 7920240,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 33,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-34-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 34,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000034_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 34,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "9dcb2ddf-2c3a-425f-9885-9e2e8a4eb95a",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:20:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 8160246,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 34,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-35-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 35,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000035_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 35,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "dd50e312-8294-46a5-adfc-b2db6729e8c8",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:24:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 8400234,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 35,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-36-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 36,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000036_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 36,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "a27c1f5b-0750-424b-ae75-bf21f00905ad",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:28:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 8640248,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 36,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-37-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 37,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000037_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 37,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "172b6023-fbc7-4954-8072-118500013163",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:32:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 8880236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 37,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-38-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 38,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000038_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 38,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "1d3aa3ca-9cc9-4dfd-9885-3bde0f99d92c",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:36:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 9120246,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 38,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-39-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 39,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000039_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 39,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "e3ee5706-07db-471b-9397-fc4c106424ff",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:40:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 9360234,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 39,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-40-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 40,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000040_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 40,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "afa9ebd6-83bc-4191-adb0-735ea724e593",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:44:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 9600236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 40,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-41-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 41,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000041_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 41,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "14747bc0-5c41-46e0-be92-6ea550669fef",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:48:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 9840238,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 41,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-42-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 42,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000042_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 42,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d31a9ebf-e32d-49cc-a4d6-25d010710d12",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:52:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 10080228,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 42,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-43-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 43,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000043_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 43,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "1a2ea5c7-3e4e-4ec8-a092-1c9f74adb380",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 18:56:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 10320249,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 43,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-44-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 44,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000044_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 44,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "959f1df0-00a6-4d6f-826a-7c152c989ee0",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:00:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 10560243,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 44,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-45-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 45,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000045_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 45,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "13f33d9e-c305-4ef2-a092-83e998e58f79",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:04:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 10800239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 45,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-46-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 46,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000046_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 46,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "aa9f674a-67a1-4c96-b09d-44e69619dad6",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:08:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 11040231,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 46,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-47-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 47,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000047_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 47,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "237113c8-5f09-4467-9b49-20b62412f786",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:12:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 11280280,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 47,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-48-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 48,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000048_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 48,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "93402dc0-3d44-44fb-b04d-84fd0535d85a",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:16:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 11520231,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 48,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-49-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 49,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000049_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 49,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6dc94372-920e-4358-a516-de318b2365d8",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:20:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 11760241,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 49,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-50-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 50,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000050_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 50,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "1dbed9a1-fd53-46e3-b6f8-569721e55879",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:24:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 12000235,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 50,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-51-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 51,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000051_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 51,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "5c1f367d-3a4b-4bef-a53d-5bcae16a54e3",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:28:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 12240239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 51,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-52-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 52,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000052_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 52,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "362dff6e-7314-447f-9a64-f10c4b072e0b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:32:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 12480228,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 52,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-53-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 53,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000053_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 53,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "cd82675f-cdf1-495d-ab29-3bef8013abb7",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:36:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 12720239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 53,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-54-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 54,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000054_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 54,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "1631b2fb-597a-488f-a66d-1c33fc6d76f7",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:40:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 12960236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 54,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-55-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 55,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000055_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 55,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "83792624-8cc5-4277-871c-1c1e7538235b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:44:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 13200231,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 55,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-56-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 56,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000056_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 56,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "c9002770-db01-4d3f-996f-c4408f471cd3",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:48:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 13440238,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 56,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-57-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 57,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000057_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 57,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "86d45a8e-8481-4c77-b040-81ce77415d9e",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:52:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 13680237,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 57,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-58-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 58,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000058_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 58,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "519bdd15-393e-4eb8-aca9-a3a1ab8f1fa7",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 19:56:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 13920242,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 58,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-59-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 59,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000059_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 59,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6f88e971-7358-44ba-9478-037d5c81bef4",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:00:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 14160229,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 59,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-60-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 60,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000060_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 60,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "027d512f-b146-4110-bc6f-523b8fc2f4a3",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:04:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 14400241,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 60,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-61-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 61,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000061_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 61,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "2622b95a-94e9-4648-9032-7b6ad2757bd5",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:08:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 14640232,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 61,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-62-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 62,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000062_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 62,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6891cc56-909a-48ba-b1e3-da5a54a48448",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:12:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 14880300,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 62,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-63-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 63,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000063_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 63,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "e88bdd42-90dc-42ee-bdc2-c5288b525deb",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:16:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 15120241,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 63,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-64-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 64,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000064_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 64,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "f86e7441-bc77-47ed-972d-c4be9acac0d2",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:20:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 15360234,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 64,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-65-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 65,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000065_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 65,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "990025b1-347a-4468-ae6d-6a82c3ca7d0f",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:24:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 15600239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 65,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-66-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 66,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000066_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 66,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "539a8497-d522-4c23-b172-5aac4fc56bfa",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:28:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 15840239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 66,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-67-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 67,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000067_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 67,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "840d1cd4-b316-4d3f-8869-98cdaf7615f9",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:32:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 16080246,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 67,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-68-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 68,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000068_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 68,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "1127675d-53f0-44c8-9dd4-5c83bf4b8dc3",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:36:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 16320243,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 68,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-69-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 69,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000069_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 69,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "4a1c59dc-b237-425a-a6ab-4ff50920cba3",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:40:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 16560235,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 69,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-70-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 70,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000070_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 70,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "2d880e69-3d2b-4673-af37-b4955ad2fb0e",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:44:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 16800236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 70,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-71-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 71,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000071_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 71,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "2cbc3332-621f-41cb-8b2c-7e82426fea1e",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:48:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 17040233,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 71,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-72-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 72,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000072_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 72,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "a55b4839-8798-4f97-ad91-3a4aa87e416a",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:52:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 17280234,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 72,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-73-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 73,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000073_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 73,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "c01245a1-8ec7-4edd-8a5f-7a2537f338d5",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 20:56:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 17520240,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 73,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-74-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 74,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000074_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 74,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "045cd3ae-c2ed-4a63-93d7-64ae282992c7",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:00:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 17760229,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 74,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-75-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 75,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000075_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 75,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "02d732ca-6bfc-4c14-9c5f-a7d0300703d5",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:04:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 18000247,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 75,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-76-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 76,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000076_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 76,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d4cd9fc7-1361-4b8f-9b16-bfd62f03e0a7",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:08:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 18240230,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 76,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-77-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 77,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000077_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 77,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "5656075e-47eb-4dd6-8e34-ec4cf1ccfb93",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:12:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 18480240,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 77,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-78-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 78,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000078_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 78,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "2515fc7a-9c18-4515-a44c-3c84f8f947ae",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:16:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 18720236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 78,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-79-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 79,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000079_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 79,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "5d9fd19a-89cb-4bc2-802f-e43a31930fb2",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:20:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 18960244,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 79,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-80-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 80,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000080_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 80,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "68563d65-9bd8-469d-8730-c25d773fdb1f",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:24:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 19200232,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 80,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-81-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 81,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000081_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 81,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d891242e-27a6-4e99-8cbc-83c11f99b906",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:28:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 19440277,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 81,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-82-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 82,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000082_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 82,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "a76b555e-c5b0-45b4-a397-3099698a1792",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:32:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 19680241,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 82,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-83-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 83,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000083_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 83,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "e46c940d-cd79-4908-a542-342f1b95d835",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:36:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 19920239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 83,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-84-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 84,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000084_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 84,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "e9bb9756-a5a8-44a3-8239-98a73f9c604f",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:40:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 20160245,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 84,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-85-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 85,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000085_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 85,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "e425275e-6560-4e9c-a952-6675f73df7f2",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:44:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 20400233,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 85,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-86-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 86,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000086_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 86,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "94f8d198-2251-4419-8c69-3401490c75af",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:48:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 20640241,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 86,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-87-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 87,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000087_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 87,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "192eecf5-e1a4-4669-bef3-c48921d34bea",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:52:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 20880248,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 87,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-88-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 88,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000088_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 88,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "aa8d738c-173c-4708-bb3a-ac7857b53502",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 21:56:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 21120499,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 88,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-89-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 89,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000089_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 89,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d155d5e7-fe1f-478b-8e2e-6bf70b782247",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:00:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 21360239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 89,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-90-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 90,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000090_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 90,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "cf4f8b56-e047-4aa4-9d75-4c3dcfaf9023",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:04:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 21600238,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 90,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-91-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 91,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000091_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 91,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "87829f46-a9a4-4152-ae01-f0dd8e86f20e",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:08:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 21840246,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 91,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-92-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 92,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000092_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 92,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "fdc3c741-95d6-4977-aa24-e793720755fd",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:12:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 22080241,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 92,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-93-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 93,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000093_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 93,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "e116d525-ad43-456e-bb41-fa5f3739c3ad",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:16:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 22320235,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 93,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-94-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 94,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000094_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 94,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6262924a-6366-412a-9816-c67b99464c3a",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:20:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 22560246,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 94,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-95-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 95,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000095_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 95,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "9cf4058e-c3e2-486b-a91b-409f1b8b3d64",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:24:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 22800240,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 95,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-96-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 96,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000096_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 96,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "beb10ce5-caad-49c0-bd55-77aa8fea907f",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:28:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 23040239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 96,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-97-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 97,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000097_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 97,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "1a774acf-ef95-468c-aadf-df1e4318b5c5",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:32:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 23280246,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 97,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-98-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 98,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000098_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 98,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "e380cbfe-9c92-4cf4-8142-8cad6549548d",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:36:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 23520240,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 98,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-99-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 99,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000099_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 99,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "7352bd5a-0374-4876-a283-86b6895b08e7",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:40:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 23760235,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 99,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-100-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 100,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000100_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 100,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "43499a0b-7f5d-4ca1-82ad-89b934cf7b2f",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:44:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 24000247,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 100,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-101-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 101,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000101_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 101,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "2987e182-d914-4a10-8487-a0c925e67a39",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:48:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 24240242,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 101,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-102-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 102,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000102_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 102,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "243e5ac6-8e3c-4936-a02b-211840eeff21",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:52:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 24480238,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 102,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-103-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 103,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000103_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 103,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6065bfe7-d072-43a8-9507-10e527c6b43d",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 22:56:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 24720232,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 103,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-104-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 104,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000104_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 104,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "2bc4db7d-a6ec-4785-bdb3-70db353233f1",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:00:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 24960243,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 104,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-105-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 105,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000105_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 105,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "876329c5-026a-41d8-a34a-60136c12a79a",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:04:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 25200238,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 105,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-106-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 106,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000106_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 106,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "0dca6b07-5f2d-45cc-9a50-260fa9c06d94",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:08:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 25440248,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 106,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-107-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 107,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000107_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 107,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "88952122-4cd3-441c-b9ec-bfb641a9835b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:12:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 25680308,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 107,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-108-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 108,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000108_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 108,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "913b276d-095c-4eba-9dbf-06d7cd6047f9",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:16:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 25920238,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 108,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-109-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 109,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000109_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 109,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "354685f6-2a8f-4d5f-9f46-6310cb0c3480",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:20:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 26160232,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 109,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-110-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 110,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000110_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 110,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "a107fcdb-4119-4e5c-8270-9dc508e15d69",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:24:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 26400243,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 110,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-111-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 111,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000111_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 111,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6eb28bb5-4bc1-4f67-b01b-efbc83b253e1",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:28:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 26640240,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 111,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-112-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 112,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000112_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 112,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "ca289e76-dbc9-4661-8106-ce0da1dabf7b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:32:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 26880235,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 112,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-113-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 113,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000113_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 113,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "a19463d0-63ce-4b3e-a9c0-1be57b990a4b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:36:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 27120244,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 113,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-114-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 114,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000114_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 114,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "26094244-3f8c-4d78-b550-3df0dc9164c3",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:40:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 27360239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 114,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-115-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 115,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000115_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 115,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "580ed785-8d96-476d-83d8-aaf6d3e4b23d",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:44:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 27600234,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 115,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-116-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 116,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000116_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 116,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "4a50e024-f119-4b30-8e79-da6bfb535451",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:48:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 27840244,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 116,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-117-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 117,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000117_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 117,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "8d8e8507-c59c-4040-b353-f32e5ccee704",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:52:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 28080239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 117,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-118-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 118,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000118_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 118,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "e08f9ecb-51ab-4a93-aa8e-ae3fe15fe2a8",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-09 23:56:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 28320237,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 118,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-119-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 119,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000119_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 119,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "71b5dcda-7706-41b4-a445-f6f4af08734b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:00:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 28560244,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 119,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-120-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 120,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000120_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 120,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "b49d81e0-1720-44e4-b5e7-1aebff1671f1",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:04:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 28800239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 120,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-121-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 121,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000121_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 121,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6cfafa4a-d165-43c7-815a-6cc5cc0366fb",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:08:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 29040235,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 121,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-122-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 122,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000122_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 122,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "34e80842-177a-4c47-adaf-553868df51c3",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:12:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 29280247,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 122,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-123-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 123,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000123_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 123,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "321ef6e6-4cb7-4b56-b4a6-79d73a0045a9",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:16:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 29520241,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 123,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-124-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 124,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000124_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 124,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "bc111e25-2ef9-4b95-8180-afdef35d7a44",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:20:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 29760236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 124,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-125-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 125,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000125_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 125,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "30deafca-d7f8-43f8-a41e-0997f9adb344",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:24:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 30000246,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 125,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-126-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 126,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000126_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 126,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "5d94f0d4-5efe-4b80-826f-fe2544fe893e",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:28:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 30240241,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 126,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-127-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 127,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000127_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 127,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "f6180f8f-4251-4e27-b8ed-48ff63c7f900",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:32:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 30480237,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 127,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-128-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 128,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000128_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 128,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "3c2974c2-9551-4ace-bb57-e3075c323a1b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:36:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 30720247,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 128,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-129-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 129,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000129_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 129,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d37d055a-5523-455f-b514-d9d36d11c9d0",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:40:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 30960243,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 129,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-130-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 130,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000130_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 130,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "0edbafc3-0359-4cb9-8dbf-cfb63f8c712f",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:44:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 31200237,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 130,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-131-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 131,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000131_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 131,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "4e0fab53-b9ef-4eb6-9b45-d7519db97ab7",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:48:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 31440233,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 131,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-132-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 132,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000132_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 132,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6bb9066a-1eb4-4515-a919-2886b64e2684",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:52:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 31680245,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 132,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-133-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 133,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000133_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 133,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "5f9b6ba9-e0c1-4c83-95bb-ce3ca5090e03",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 00:56:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 31920240,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 133,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-134-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 134,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000134_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 134,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "13cdcd48-a1fb-4831-be2c-eedb19a0ffe3",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:00:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 32160236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 134,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-135-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 135,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000135_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 135,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "b397fd07-cc7c-41b8-b61c-1c4aa881d6da",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:04:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 32400245,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 135,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-136-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 136,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000136_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 136,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "388a2ff7-8c66-4218-89b6-caf6edcc8867",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:08:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 32640240,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 136,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-137-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 137,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000137_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 137,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "549141f2-8474-434b-9740-73b83c192764",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:12:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 32880234,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 137,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-138-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 138,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000138_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 138,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "9c5698e3-4631-4035-99ae-a73c11dec81d",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:16:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 33120246,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 138,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-139-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 139,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000139_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 139,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "f5942b38-0483-4813-9616-ce4ab94b4070",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:20:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 33360240,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 139,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-140-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 140,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000140_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 140,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "cb430db8-b443-4638-bc64-3f68e54dc997",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:24:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 33600238,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 140,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-141-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 141,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000141_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 141,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d79bc682-3c62-4f59-8c1f-28001801d419",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:28:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 33840246,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 141,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-142-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 142,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000142_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 142,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "64af51e0-b5ba-4318-9a28-9f173918cdb0",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:32:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 34080241,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 142,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-143-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 143,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000143_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 143,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "30613539-a22a-4eec-ac7f-af0732cdbd5b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:36:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 34320236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 143,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-144-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 144,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000144_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 144,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6a08f410-1e1f-4fef-afdb-a1aa4564c257",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:40:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 34560246,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 144,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-145-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 145,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000145_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 145,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "87468a9e-f34b-4f60-836b-47d0d398583f",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:44:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 34800242,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 145,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-146-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 146,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000146_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 146,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "9702786e-576f-4f56-8b74-7821e082e6ca",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:48:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 35040235,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 146,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-147-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 147,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000147_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 147,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "509a20ca-f261-431c-a12c-7e613e205cea",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:52:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 35280247,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 147,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-148-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 148,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000148_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 148,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d5fab5b5-3e92-4325-9cbd-0145f67aa7fe",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 01:56:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 35520242,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 148,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-149-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 149,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000149_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 149,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "21e46117-77ef-41f6-a8be-b6c4f9a19b29",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:00:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 35760236,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 149,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-150-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 150,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000150_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 150,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "beca7290-87b4-4e2f-a644-84da9a711d81",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:04:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 36000247,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 150,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-151-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 151,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000151_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 151,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "2d6c1179-98d6-47ea-baa6-da3f82413a8c",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:08:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 36240242,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 151,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-152-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 152,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000152_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 152,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "410227bc-435f-4c3d-85a8-95bcb30c8e26",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:12:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 36480238,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 152,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-153-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 153,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000153_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 153,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "3db7f3dc-bdcf-4714-a5de-2547abe9f3ee",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:16:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 36720232,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 153,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-154-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 154,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000154_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 154,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d6393b3d-3fa3-4195-ae17-623553188464",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:20:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 36960244,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 154,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-155-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 155,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000155_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 155,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "cd590742-1f1e-45ac-8be8-614e9eb224ed",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:24:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 37200239,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 155,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-156-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 156,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000156_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 156,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "81dfab3e-f301-4b27-999a-ba7568454c7f",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:28:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 37440249,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 156,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-157-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 157,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000157_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 157,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "b13a7b89-ecc3-4c46-bfd7-55824f317984",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:32:39 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 37680244,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 157,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-158-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 158,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000158_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 158,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "1d6aa97c-1971-4d4a-ae16-03c7bc9b14fa",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:37:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 37943821,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 158,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-159-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 159,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000159_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 159,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "f837a630-e82f-4e59-9144-cd7c5c5fced0",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:41:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 38183589,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 159,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-160-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 160,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000160_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 160,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d836d07d-17a8-4bf3-a264-b75bf422ec0f",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:45:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 38423599,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 160,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-161-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 161,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000161_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 161,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "91d5c2a4-4b81-48ac-8300-317ab175d6cd",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:49:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 38663594,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 161,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-162-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 162,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000162_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 162,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "cf4997f2-c5a2-45d4-b841-8fcc8791b4ae",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:53:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 38903592,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 162,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-163-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 163,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000163_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 163,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "6b45e212-1837-498c-972e-fb427a79ab77",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 02:57:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 39143587,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 163,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-164-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 164,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000164_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 164,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "a2c61a15-73bf-4438-8ac9-8160627f3ac0",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:01:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 39383598,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 164,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-165-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 165,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000165_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 165,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "f731e1cb-da43-4c2a-8951-05f0be4145c8",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:05:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 39623594,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 165,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-166-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 166,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000166_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 166,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "9f9ddcbe-c12a-46b6-9793-c82fb7ade620",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:09:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 39863590,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 166,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-167-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 167,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000167_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 167,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "e31955f5-766c-4b11-93fd-fbf8e2371d58",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:13:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 40103600,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 167,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-168-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 168,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000168_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 168,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "52501c37-769f-4595-b3f5-5c43e62d77c2",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:17:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 40343596,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 168,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-169-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 169,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000169_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 169,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "b174d47e-4fe5-43f7-afbf-f2c3365f53ec",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:21:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 40583594,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 169,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-170-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 170,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000170_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 170,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "40df50dd-e586-4722-afaa-7e1e3eb35ad9",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:25:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 40823590,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 170,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-171-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 171,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000171_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 171,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "c6d57fa8-a795-42cf-8e6b-062e368161a0",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:29:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 41063601,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 171,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-172-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 172,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000172_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 172,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d380aa1e-13c5-4a88-a46a-6611322fc7ee",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:33:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 41303598,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 172,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-173-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 173,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000173_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 173,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "137f3ed7-0ff5-4c72-b95e-22e6f20b81e9",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:37:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 41543594,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 173,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-174-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 174,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000174_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 174,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "a689b08b-1c02-43c4-b6bf-179d04378184",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:41:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 41783587,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 174,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-175-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 175,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000175_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 175,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "3b743608-ce9e-4196-a4b2-ae0c03cae20c",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:45:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 42023600,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 175,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-176-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 176,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000176_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 176,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "d4ad8c35-2d1e-475a-925a-82590f3a53c7",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:49:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 42263596,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 176,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-177-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 177,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000177_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 177,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "80b38374-7dc8-4dbe-b7f5-eae65fcc063e",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:53:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 42503593,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 177,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-178-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 178,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000178_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 178,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "134186f1-2841-4bd7-9f08-2b5de3a8a21b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 03:57:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 42743588,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 178,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-179-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 179,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000179_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 179,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "4673b96a-a436-44cc-b517-168fb715efa6",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:01:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 42983600,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 179,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-180-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 180,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000180_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 180,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "7a53ad8f-2f49-42b8-9659-da6b614ed6fd",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:05:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 43223596,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 180,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-181-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 181,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000181_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 181,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "ccf3ba83-dbb8-4108-9023-cbf726cc3b21",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:09:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 43463591,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 181,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-182-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 182,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000182_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 182,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "a5088ad8-8b97-405b-b328-372f55fbec78",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:13:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 43703603,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 182,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-183-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 183,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000183_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 183,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "c7114176-416c-489c-bb7a-9bb832196ab9",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:17:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 43943598,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 183,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-184-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 184,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000184_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 184,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "69652d91-1e1f-4cd9-a7d7-1cf3c4b78464",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:21:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 44183594,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 184,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-185-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 185,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000185_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 185,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "3d3ecc1e-d3e8-4190-a525-c6c324c48be4",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:25:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 44423590,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 185,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-186-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 186,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000186_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 186,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "4042ed32-688a-4e8f-bdec-ef2a12dccb56",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:29:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 44663601,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 186,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-187-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 187,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000187_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 187,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "28889f7f-e66e-4791-a2a5-ada80ed8d59b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:33:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 44903597,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 187,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-188-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 188,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000188_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 188,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "2e222892-322a-407f-94c6-307d11983a99",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:37:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 45143593,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 188,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-189-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 189,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000189_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 189,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "02d57f82-3f43-4c37-a546-37b515c9d8f9",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:41:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 45383866,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 189,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-190-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 190,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000190_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 190,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "0f894813-5621-4455-ab51-f8e7919b680b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:45:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 45623588,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 190,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-191-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 191,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000191_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 191,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "886328c9-4f39-4114-9344-1fee4307883f",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:49:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 45863591,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 191,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-192-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 192,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000192_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 192,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "a7eb23e3-8c6b-49a5-b885-ebe288297af9",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:53:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 46103613,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 192,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-193-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 193,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000193_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 193,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "1e802dfb-90b9-4cae-9bb4-d241ea2f9546",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 04:57:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 46343604,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 193,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-194-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 194,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000194_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 194,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "ce1e4dda-540f-4255-86a0-660642d5252a",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 05:01:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 46583602,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 194,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-195-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 195,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000195_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 195,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "ac91920a-bdbf-4b7b-8662-b2a1905eac75",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 05:05:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 46823878,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 195,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-196-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 196,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000196_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 196,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "5e12be84-32ba-4c1a-9c99-cfe0fab4bdb2",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 05:09:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 47063593,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 196,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-197-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 197,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000197_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 197,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "1cd762a9-6f1f-458c-aa81-f8da6c4379e7",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 05:13:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 47303589,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 197,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-198-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 198,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000198_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 198,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "660d585b-2169-486a-ac0f-a3cb5bcbc295",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 05:17:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 47543601,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 198,
"Andor Clara CCD-ReadoutTime": "86.52"
},
"FrameKey-199-0-0": {
"COM3-Verbose": "1",
"Andor Clara CCD-Shutter Opening Time": "20",
"Light path-Label": "2-Left100",
"Core-Shutter": "",
"Filter turret-Name": "TIFilterBlock1",
"Nikon Ti-E-FirmwareVersion": "Ti-E V2.10",
"COM3-Description": "Serial port driver (boost:asio)",
"Filter turret-State": "3",
"Andor Clara CCD-Advanced | Snap Image Additional Delay (ms)": "0",
"ZPositionUm": 9160.150136496872,
"ChannelIndex": 0,
"Core-AutoShutter": "0",
"Andor Clara CCD-PixelType": "16bit",
"Exposure-ms": 20,
"Andor Clara CCD-1. Camera Information : | Type | Model | Serial No. |": "| Clara | DR328_C01~G | 1474 |",
"SliceIndex": 0,
"Position": "Default",
"Andor Clara CCD-Overlap": "Off",
"CameraChannelIndex": 0,
"Andor Clara CCD-Camera": "| Clara | DR328_C01~G | 1474 |",
"Prior XY stage-MaxSpeed": "100",
"AxisPositions": null,
"Andor Clara CCD-ReadoutMode": "1.000 MHz",
"Z drive-Name": "TIZDrive",
"Light path-State": "1",
"Prior XY stage-SCurve": "100",
"Andor Clara CCD-TransposeXY": "0",
"PixelSizeUm": 0.16082474,
"Core-ChannelGroup": "Filter",
"Frame": 199,
"Andor Clara CCD-ReadMode": "Image",
"PFS Z offset-Name": "TIPFSOffset",
"Prior XY stage-Port": "COM3",
"Core-ImageProcessor": "",
"FileName": "img_000000199_4 - Empty_000.tif",
"Andor Clara CCD-KeepCleanTime": "0.01",
"Core-Camera": "Andor Clara CCD",
"Prior XY stage-Description": "Prior XY stage driver adapter",
"PFS status-FullFocusTimeoutMs": "5000.0000",
"Light path-ExtraDelayMs": "0.0000",
"Andor Clara CCD-Region of Interest": "Full Image",
"Andor Clara CCD-ActualInterval-ms": "106.53",
"COM3-StopBits": "1",
"Andor Clara CCD-VerticalSpeed": "6.50",
"Andor Clara CCD-FanMode": "Full",
"Andor Clara CCD-SpuriousNoiseFilterDescription": "Spurious Noise Filter is Off",
"Filter turret-Label": "4-UV-2A",
"Width": 1392,
"Prior XY stage-StepSizeY_um": "1.0000",
"Objective turret-Name": "TINosePiece",
"Andor Clara CCD-SpuriousNoiseFilterThreshold": "0.0000",
"Prior XY stage-Acceleration": "100",
"Andor Clara CCD-Description": "Andor camera adapter",
"Andor Clara CCD-TimeOut": "10000",
"COM3-DataBits": "8",
"Prior XY stage-TransposeMirrorY": "0",
"Prior XY stage-TransposeMirrorX": "0",
"Nikon Ti-E-SoftwareVersion": "4.4.1.714",
"Nikon Ti-E-DeviceAddress": "USB_DEVICE(1)",
"Andor Clara CCD-CoolerMode": "Fan off at shutdown",
"WaitInterval": 240000,
"Filter turret-ExtraDelayMs": "0.0000",
"YPositionUm": -28090,
"Andor Clara CCD-TransposeCorrection": "0",
"Andor Clara CCD-Exposure": "20.00",
"COM3-BaudRate": "9600",
"Andor Clara CCD-Isolated Crop Mode": "Off",
"NextFrame": 199,
"Andor Clara CCD-Binning": "1",
"Core-Initialize": "1",
"BitDepth": 14,
"Andor Clara CCD-CCDTemperature": "1",
"Nikon Ti-E-DriverVersion": "micusb.sys 2.0.5.1",
"Z drive-Tolerance": "0",
"Andor Clara CCD-AD_Converter": "2. 14bit",
"Andor Clara CCD-Output_Amplifier": "Normal",
"Core-AutoFocus": "PFS status",
"Objective turret-Label": "4-Plan Fluor 40x NA 0.75 Dry",
"Camera": "",
"UUID": "864ee5d2-d909-4930-8077-42ea31f9da3b",
"Andor Clara CCD-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
"PositionName": "Pos0",
"COM3-Handshaking": "Off",
"Channel": "4 - Empty",
"Core-TimeoutMs": "5000",
"ROI": "0-0-1392-1040",
"COM3-Parity": "None",
"Slice": 0,
"SlicePosition": null,
"Andor Clara CCD-Overlap Help": "Should only turn on Overlap if using Burst or Live mode.",
"Andor Clara CCD-TransposeMirrorY": "0",
"Andor Clara CCD-TransposeMirrorX": "0",
"PFS status-Status": "Locked in focus",
"Core-Galvo": "",
"Andor Clara CCD-Shutter TTL Value": "High to Open",
"Andor Clara CCD-Advanced | Snap Image Timing Mode": "Delay for Exposure",
"Andor Clara CCD-Trigger": "Software",
"Andor Clara CCD-SpuriousNoiseFilter": "None",
"Andor Clara CCD-Shutter": "Open",
"Time": "2017-02-10 05:21:03 +0100",
"Objective turret-State": "3",
"PFS Z offset-Position": "416.10000000000002",
"Source": "Andor Clara CCD",
"Z drive-Speed": "1",
"Light path-Name": "TILightPath",
"Binning": "1",
"Core-XYStage": "Prior XY stage",
"PFS status-Name": "TIPFSStatus",
"XPositionUm": -18138,
"Height": 1040,
"COM3-AnswerTimeout": "500.0000",
"PixelType": "GRAY16",
"COM3-DelayBetweenCharsMs": "0.0000",
"Nikon Ti-E-DeviceIndex": "1",
"PFS status-FullFocusWaitAfterLockMs": "100.0000",
"Objective turret-ExtraDelayMs": "0.0000",
"Core-SLM": "",
"Andor Clara CCD-CCDTemperatureSetPoint": "0",
"ElapsedTime-ms": 47783823,
"COM3-Name": "COM3",
"Andor Clara CCD-Pre-Amp-Gain": "2x",
"Andor Clara CCD-Shutter Closing Time": "20",
"Core-Focus": "Z drive",
"PFS status-State": "On",
"Prior XY stage-StepSizeX_um": "1.0000",
"Summary": {
"Slices": 1,
"ChContrastMin": [0],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 240000,
"Time": "2017-02-09 16:04:41 +0100",
"Prefix": "test-13h_1",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28090
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28278
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18138,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18336,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18552,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"Prior XY stage": [
-18832,
-28457
],
"PFS Z offset": [416.1]
},
"GridColumnIndex": 0
}
],
"z-step_um": 0,
"GridRow": 0,
"BitDepth": 14,
"Depth": 2,
"Channels": 1,
"Source": "Micro-Manager",
"ChColors": [-1],
"MetadataVersion": 10,
"KeepShutterOpenChannels": false,
"ChContrastMax": [65536],
"CustomIntervals_ms": [],
"Height": 1040,
"MicroManagerVersion": "1.4.22",
"GridColumn": 0,
"UUID": "f2917f90-7f3d-4b2c-b885-44ee06c5d76e",
"PixelType": "GRAY16",
"Width": 1392,
"Directory": "C:\\Users\\Icy\\Desktop\\Conidies",
"Frames": 200,
"Comment": "",
"UserName": "Icy",
"PixelSize_um": 0.16082474,
"ROI": [
0,
0,
1392,
1040
],
"Date": "2017-02-09",
"ChNames": ["Default"],
"KeepShutterOpenSlices": false,
"IJType": 1,
"ComputerName": "Microscope",
"Positions": 6,
"PositionIndex": 5,
"TimeFirst": false
},
"PositionIndex": 0,
"FrameIndex": 199,
"Andor Clara CCD-ReadoutTime": "86.52"
}
}
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