[ome-users] getPlanePositionZ for z-stage AND z-piezo from ND2 file
William Moore (Staff)
W.Moore at dundee.ac.uk
Wed Aug 31 11:47:26 BST 2016
If you try opening your files in ImageJ/Fiji with the Bio-formats plugin and choose to
“Display Metadata” and “Display OME-XML metadata”, do you see anything there
that represents the z info you need?
It would be really helpful if you could send us a sample ND2 file that has this metadata.
Then we can check if / where Bio-Formats is reading it.
Please let us know what values you’re expecting for the z-stage and z-piezo once you’ve
uploaded the file so we know what to look for.
You an upload files at https://www.openmicroscopy.org/qa2/qa/upload/
On 30 Aug 2016, at 12:00, Enno R. Oldewurtel <eoldewur at pasteur.fr<mailto:eoldewur at pasteur.fr>> wrote:
maybe someone can help me retrieving the metadata for z-positions from an ND2 file.
I have been using the following in the past:
But now I work on a Nikon microscope that has both a z-stage and a z-piezo. How can I retrieve the two z values? At the moment, getPlanePositionZ(i,j) gives me the position of the piezo.
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