[ome-users] Imspector msr FLIM

[Ewan McGhee]

Hi,

I believe I’ve encountered a problem trying to use the raw msr files produced by our LaVision Biotec Trim-scopes – specifically, the FLIM data that we acquire.

There seems to be two separate problems:

1. The data taken on the old Trim-scope which runs 32-bit Imspector and uses the LaVisionBiotec x16-TCSPC detector, loads into FLIMFit but the time axis used for measuring the decay is wrong (it shows as 0 to 1 ps, when it should be 0 to 12 ns). The one-tiff export produced by Imspector of the same data, however, loads fine with the correct time axis values.

2. The new Trim-scope we have runs the 64-bit version of Imspector as we have the new hybrid PMT FLIM detectors on this. The msr file produced form this system just crashes out completely -

Error using flim_data_series/get_image_dimensions (line 108)
Java exception occurred:
java.lang.ArithmeticException: / by zero
at loci.formats.in.ImspectorReader.initFile(ImspectorReader.java:421)
at loci.formats.FormatReader.setId(FormatReader.java:1426)
at loci.formats.ImageReader.setId(ImageReader.java:835)
at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:651)
at loci.formats.ChannelFiller.setId(ChannelFiller.java:223)
at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:651)
at loci.formats.ChannelSeparator.setId(ChannelSeparator.java:289).

I have uploaded an example of each data file to https://www.openmicroscopy.org/qa2/qa/upload/, with the detector type indicated in the file name: 01 - 770nm FITC - HybridPMT.msr and 02 - 70 - 1 - tp1 - x16 TCSPC.msr.

If I can help any other way just let me know.

Cheers,

Ewan
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