[ome-users] Bio-Formats plug-in unable to open EasyRatio Pro files

Sebastien Besson (Staff) s.besson at dundee.ac.uk
Mon Aug 3 14:46:33 BST 2015

Hi Nickolai,

Bio-Formats supports files generated by ImagePro ending with the `.seq` extension. [1]
Unless EasyRatio Pro uses the same writing framework as ImagePro, Bio-Formats is
unlikely to support this image format. Is there an alternate format you can use to export
the imaging data from EasyRatio Pro?

Alternatively, if you want to request support for the EasyRatio Pro format in Bio-Formats,
you can upload some sample data to http://qa.openmicroscopy.org.uk/qa/upload/.


[1] https://www.openmicroscopy.org/site/support/bio-formats5.1/formats/imagepro-sequence.html

On 31 Jul 2015, at 10:22, Vysokov, Nickolai <nickolai.vysokov at kcl.ac.uk<mailto:nickolai.vysokov at kcl.ac.uk>> wrote:

Hi All

I am collecting my raw Ca2+ Imaging data in a software called EasyRatio Pro. It generates a session “.esf” file and “.seq” files containing the actual images (it looks like they are essentially TIF files intertwined with some metadata). The bio-formats support page says that .seq files are supported, but I cannot get FIJI to open them (I would like to use ImageJ macros for image processing and analysis). Is this a bug or the Bio-Formats never meant to open the .seq files from EasyRatio Pro? If latter is the case, then should I contact the Photon Technology International and ask for this feature (I cannot imagine it difficult to implement) or this is all within the hands of OME users?

Any advice would be greatly appreciated!

Yours faithfully,
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