[ome-users] Crop on import with Nikon ND2 image files - Only works with x-origin=1

[Enno R. Oldewurtel]

Dear all,

I just tried to read in ND2 files via matlab (either using r.openBytes
directly or bfopen.m). However, when specifying a sub-image with
x-origin>=2, the resulting image is skewed along a diagonal with slope
depending on the x-origin, i.e. slope=1 for x-origin=2. The y-origin works
with all values.

This is the command I use:
test=bfopen(f,2,1,400,300);

Using bfopen on a tif-file returns a correctly cropped image. Thus this
might be a nd2 specific problem.

Has anyone experienced the same problem?

These are images obtained with x-origin=1 and x-origin=2:[image: Inline
image 1][image: Inline image 2]

Thanks,
Enno



-- 
Enno R. Oldewurtel
AG Prof. B. Maier / Biophysics Group
Biozentrum
Universitaet zu Koeln
Zuelpicher Str. 47b
50674 Koeln

enno.oldewurtel at uni-koeln.de
http://www.biophysics.uni-koeln.de/
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