[ome-users] missing images reading Nikon nd2 files

Melissa Linkert melissa at glencoesoftware.com
Thu Jul 17 23:28:23 BST 2014


Hi Karsten,

> Roger, I have to correct, the uploaded dataset ...MM63_C10h has after NIS Elements Viewer 105 rgb images, not 106. One, the last image is missing and that is always the case. 
> 
> NIS don't show me any series and we don't know where either series or t/z sequences appear. Also my colleague states having saved the files without any known changes!

Thank you for uploading example datasets.  We now have a proposed fix
for this problem:

https://github.com/openmicroscopy/bioformats/pull/1226

which I expect to be included in the upcoming 5.0.3 release.

Regards,
-Melissa

On Wed, Jul 16, 2014 at 01:36:09PM +0200, Karsten wrote:
> 
> Roger, I have to correct, the uploaded dataset ...MM63_C10h has after NIS Elements Viewer 105 rgb images, not 106. One, the last image is missing and that is always the case. 
> 
> NIS don't show me any series and we don't know where either series or t/z sequences appear. Also my colleague states having saved the files without any known changes!
> 
> Regards
> 
> Karsten
> 
> Again with better subject
> 
> Am 16.07.2014 um 13:00 schrieb ome-users-request at lists.openmicroscopy.org.uk:
> 
> > For the latter image I see:
> > 
> > x = 1280
> > y = 960
> > c = 3
> > series = 104 (0..103)
> > 
> > It looks like it's missing the last two planes?  So it's definitely seen
> > as 106 series in Nikon Elements?  Are there any other problems here
> > relating to the image dimensionality e.g. for channels or z/t?
> 
> Karsten
> karo13de at googlemail.com
> 
> 
> 

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