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h.flynn at dundee.ac.uk
Fri Feb 21 18:15:13 GMT 2014
I am replying cc'd to our users list which is the proper place to ask Bio-Formats related questions.
The information on what formats Bio-Formats supports is on
If you click on the format type you have in this table, you will find more information about how well each format is supported which should answer your question about the transformation.
We are hoping to release an updated version of the Bio-Formats plugin early next week but in the meantime, you can download the 'loci-tools.jar' from http://downloads.openmicroscopy.org/bio-formats/5.0.0-rc1/
and use the documentation on http://www.openmicroscopy.org/site/support/bio-formats5/users/imagej/installing.html and http://www.openmicroscopy.org/site/support/bio-formats5/users/imagej/load-images.html to guide you through getting it installed and loading your images.
If you need any further help, please reply to the mailing list and someone will get back to you next week,
On 21 Feb 2014, at 17:53, Peter Gerondelis <peter.z.gerondelis at gsk.com<mailto:peter.z.gerondelis at gsk.com>> wrote:
Thank you for contacting us.
Please provide as much information as possible so we can deal with your enquiry efficiently.
Hi, I am looking to use ImageJ to analyze phosphorimaging files from my Typhoon and Storm. I can no longer use the software provided by the manufacturer (formerly Molecular Dynamics, then Amersham and now GE). My understanding is that these are not ordinary TIFF files. They are transformed by the instrument and/or software. Someone from the ImageJ list serve suggested that I try Bio Formats plugin. I thought I would try it but can’t figure out how to install. Before I put any additional energy to getting this plugin and installing it, I would like to confirm that the plugin does more than just open the file, but does it handle the transformation correctly? If so, can you explain to me which file I need to download from the website, how to get it properly installed into ImageJ and how to use it????
Please note the following related post from the ImageJ list serve:
Amersham .gel file warning<https://list.nih.gov/cgi-bin/wa.exe?A2=IMAGEJ;9ad542ec.0512>
Morri Feldman <[log in to unmask]<https://list.nih.gov/cgi-bin/wa.exe?LOGON=A2%3Dind0512%26L%3DIMAGEJ%26P%3DR16807%261%3DIMAGEJ%269%3DA%26I%3D-3%26J%3Don%26d%3DNo%2BMatch%253BMatch%253BMatches%26z%3D4>>
ImageJ Interest Group <[log in to unmask]<https://list.nih.gov/cgi-bin/wa.exe?LOGON=A2%3Dind0512%26L%3DIMAGEJ%26P%3DR16807%261%3DIMAGEJ%269%3DA%26I%3D-3%26J%3Don%26d%3DNo%2BMatch%253BMatch%253BMatches%26z%3D4>>
Tue, 20 Dec 2005 11:54:00 -0800
text/plain<https://list.nih.gov/cgi-bin/wa.exe?A3=ind0512&L=IMAGEJ&E=8bit&P=698804&B=--&T=text%2Fplain;%20charset=ISO-8859-1&header=1> (29 lines)
The Amersham .gel file format is based on the tiff standard. ImageJ
will read .gel files because they are tiff files. Unfortunately
Amersham scanners (e.g. Typhoon and Storm) transform the original
pixel values by taking their square root and scaling them before
saving them to a .gel file. When imagej opens a .gel file, it doesn't
reverse this transformation making the images appear muted and
unsuitable for quantification.
Here are some links.
A short note about Amersham's .gel file format:
A pdf document from when Molecular Dynamics owned the .gel file format:
If the information in the pdf is current, it should be possible to
read a few private tiff tags from the .gel file and use their values
to reverse the image transformation. Barring this solution, be wary
of quantifying data directly from .gel files.
UCSF Biophysics Program
Peter Gerondelis, Ph.D.
RD Infectious Disease R&D
5 Moore Drive, PO Box 13398, RTP, NC 27709-3398, United States
Email peter.z.gerondelis at gsk.com<mailto:peter.z.gerondelis at gsk.com>
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