[ome-users] Omero: Import RGB image

[Will Moore]

Hi Veronique,

  I'm not sure what is going on here.

I just tried importing your attached file and I got the same error as  
you (bright red channel).
But when I import other jpgs I get no problem.

If I open your jpg and save it as a jpg or tiff (using Preview on  
Mac) then it imports OK.
The red and blue channels have a little noise (due to jpg compression)?
But no bright red channel.

So, I'm not sure why your green image is importing strangely.

I'm just wondering why you are converting grey images into rgb before  
import, since you are only using 1 channel of the rgb.
Why don't you simply import the grey-level image?
You can then set the rendering in Insight to change the appearance of  
the image if you wish.


   Will.


On 7 Jul 2009, at 13:13, V. Mallouh wrote:

> Hello,
>
> Please find below the description of our problem during the import  
> of a RGB image in Omero (4.0.3).
>
> We have generated (with ImageJ) RGB images by putting the original  
> grey-level image in one of the RGB channels (according  
> fluorochrome), the two other channels kept blank (greylevel=0).
>
> Importing these RGB images in Omero is working fine when datas are  
> is in the Red or in the Blue channel.
> But in the  case where:
>                       Green channel is containing the datas
>                       Red and Blue channels kept blank
> then the display of the Red channel is wrong (bright red instead of  
> black), resulting in a red colored RGB image.
>
> The same occurs with tiff or ome-tif files.
> You will find the RGB image attached to this mail.
>
> Thanks for your support,
>
> Véronique Mallouh
> IGBMC
> 1 Rue Laurent Fries
> 67404 Illkirch Cedex
> France
> mallouh at igbmc.fr
>
> <RGB-pb.jpg>
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> ome-users mailing list
> ome-users at lists.openmicroscopy.org.uk
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William Moore
Division of Gene Regulation and Expression
College of Life Sciences
University of Dundee
Scotland
DD1 5PH

Tel 01382 386364

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