[ome-devel] smart way to open a time series of one pixel?

Grabmayr, Heinrich Heinrich.Grabmayr at ph.tum.de
Fri Feb 6 20:05:15 GMT 2015


Hi,

I have huge movies (20k to 60k frames) in file formats from various camera suppliers that can all be opened using bioformats. I would like to access the time series of indivindual pixels. At the moment, I do this in matlab by using
r = bfGetReader
and then using the loci.formats.ChannelSeparator's function to call
r.openBytes<http://downloads.openmicroscopy.org/bio-formats/5.0.6/api/loci/formats/ChannelSeparator.html#openBytes%28int,%20int,%20int,%20int,%20int%29>(int no, int x, int y, int w, int h) with w=h=1 and looping through all the planes. This takes ages. I was wondering whether there might be a smarter way to access the data I am interested in?

Thanks very much
   Heinrich
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