[ome-devel] Storage format for 'fitted' parameter (FLIM) images?
Melissa Linkert
melissa at glencoesoftware.com
Tue Mar 13 15:05:33 GMT 2012
Hi Ian,
> I've finally managed to get back to looking at this problem & in the absence of any feedback on here,
> I've started looking at openEXR as an open floating-point image format for holding processed parameter images.
>
> As far as I can tell this is already compatible with bio-formats.
I can't speak to the suitability of OpenEXR for storing FLIM data, but
do note that OpenEXR files are not currently supported in
OMERO/Bio-Formats. There are plans to support these files, as noted here:
http://trac.openmicroscopy.org.uk/ome/ticket/4125
...but the work has not been done yet, and realistically will not be
done until after the 4.4 release of OMERO/Bio-Formats.
Regards,
-Melissa
On Tue, Mar 13, 2012 at 02:20:18PM +0000, Munro, Ian wrote:
> Hi Again.
>
> I've finally managed to get back to looking at this problem & in the absence of any feedback on here,
> I've started looking at openEXR as an open floating-point image format for holding processed parameter images.
>
> As far as I can tell this is already compatible with bio-formats.
>
> Does anyone have any thoughts or objections?
>
> Regards
>
>
> Ian
>
>
> On 22 Feb 2012, at 12:16, wrote:
>
> > HI all
> >
> > At the recent Dundee meeting I described 3 categories of data required to make OMERO compatible with
> > fluorescence lifetime imaging (FLIM).
> >
> > 1) 'raw' time-resolved intensity data (as acquired by the microscope)
> > 2) fitted (floating point) parameter 'images' (generated by fitting software)
> > 3) merged RGB maps. (for inclusion in papers etc)
> >
> > Currently the 2nd type is stored as a .csv image by the fitting code that Donald has already included in OMERO.
> > There is concern here over this for two reasons:
> >
> > 1) iIt is inefficient in terms of storage.
> > 2) There will inevitably be a loss of precision.
> >
> > Could we change to another format for this (assuming it isn't too late) ?
> > If so what format?
> >
> > Any thoughts welcome.
> >
> > Regards
> >
> > Ian
>
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