[lm-announce] FW: Quantitative Imaging course at CSHL

[Samuel Swift]

Dear All,

Please see below for details of the 2015  Quantitative Imaging course at Cold Spring Harbour.  More information can be found at: http://meetings.cshl.edu/courses/2015/c-qicm15.shtml

Best,
Sam



From: Waters, Jennifer Catherine [mailto:jennifer_waters at hms.harvard.edu]
Sent: Friday, November 14, 2014 5:09 PM
To: Jason Swedlow
Subject: Quantitative Imaging course at CSHL

Dear Jason,

I wanted to let you know about a quantitative microscopy course that Torsten Wittmann (UCSF), Hunter Elliott (Harvard) and I are organizing at Cold Spring Harbor Laboratory, called "Quantitative Imaging: From Cells to Molecules".  We will be teaching the course for the fourth time in April 2015, with applications due January 31, 2015.  Our course has received excellent reviews from the students, and we feel it has been a great success.  We accept only 16 students, and we have excellent support from vendors in the form of equipment loans - so each student gets lots of hands-on time with state-of-the-art microscopes during the labs.  We also emphasize quantitation throughout the course, both in lectures and in daily quantitative lab exercises.  If you are interested in educating your students or post-docs in quantitative imaging, we hope you will encourage them to apply.  The course is supported by a grant from NCI, so Cold Spring Harbor is able to offer financial aid to many students to help cover tuition costs.

We would also appreciate if you could hang up the attached poster, and forward this email to any colleagues you think might be interested.

Course Description:
This course will focus on advanced quantitative fluorescence microscopy techniques used for imaging a range of biological specimens, from cells to single molecules. The course is designed for cell and molecular biologists with little or no microscopy experience who wish to begin utilizing microscopy in their own research. Students will gain a theoretical understanding of, and hands-on experience with, state-of-the-art equipment used in quantitative fluorescence microscopy, including: wide-field fluorescence microscopy, laser scanning and spinning disk confocal microscopy, total internal fluorescence microscopy (TIRF), super-resolution methods (structured illumination, STED, STORM and PALM) and digital image processing and analysis. Students will learn how to design and implement a wide range of imaging experiments using these techniques. Students will use the techniques to address specific quantitative questions and then discuss the results as a group, learning to troubleshoot the common problems that occur in the course of a quantitative imaging experiment. Among the lectures presented are: quantitative microscopy basics, transmitted light microscopy, image segmentation, image analysis, CCD & sCMOS cameras, confocal microscopy, multi-photon microscopy, deconvolution, TIRF, imaging ratio-metric "biosensors" (including FRET), light sheet microscopy and super-resolution techniques. Students will also learn guidelines for choosing fluorescent proteins, and work with live samples requiring environmental control.

Instructors & Lecturers:  Jennifer Waters (Harvard), Torsten Wittmann (UCSF), Hunter Elliott (Harvard), Nathan Shaner (The Scintillon Institute), Dylan Burnette (Vanderbilt) & Pavel Tomancak (Max Planck Institute)

More info and online application:  http://meetings.cshl.edu/courses/2015/c-qicm15.shtml

Best, Jennifer
~~~~~~~~~~~~~~~
Jennifer C. Waters, Ph.D.
Director of Microscopy

Harvard Medical School
Dept of Cell Biology
240 Longwood Ave
Boston, MA 02115
P: (617) 432-3542
E: jennifer_waters at hms.harvard.edu<mailto:jennifer_waters at hms.harvard.edu>




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