[lm-announce] molecular probes workshop - update

[Sam Swift]

Dear All,

I am very surprised to hear that there are still places left on the  
molecular probes workshop.  Paul has provided an update to the  
program (see below).  This should appeal to anybody using or wishing  
to use the Alexa dyes and, more generally, is a valuable and rare  
opportunity to improve your understanding of dye chemistry and the  
use of fluorophores in fluorescence microscopy.  Please contact Paul  
to book your place.

Cheers,
Sam



Hi,

All those that have already replied are welcome to attend, plenty of  
spaces left .


A slightly more final programme :


Friday 20th June

10.30am - 1.45pm

Front Seminar Room WTB

Presented by:

Nick Dolman: Molecular Probes Scientist
Annemieke Walker: Our Invitrogen Cell Biology Technical Sales Specialist

1030 am. Fixed cell stuff.~30 minutes
Intro about Mol Probes.
Optimising imaging-check list for getting the best image
Alexa Fluor dyes PLUS picking a good primary!!
Anti fade reagents
Reagents to block non specific binding
Nuclear dyes
Questions

1115: Novel Assays/Nucleic acid/protein/saccharide synthesis. ~30  
minutes
Click IT EdU.
Click IT AHA
Click IT for glycoproteins
Start on quick explanation of Click chemistry
Applications
Advantages
Questions

1200: Live Cell. ~30 minutes
Organelle lights/BacMam/ion channels
Comparison of Organelle lights and organic organelle stains.
Sensors….
Novel sensors and other ions (Premo Cameleon, FluxOR), other ion  
channels mainly R & D stuff
Questions

1230 – 1345 Trouble shooting Q & A session
Your chance to ask the experts how to get the best from their products.

For further information contact Paul Appleton p.l.appleton at dundee.ac.uk