[FLIMfit-users] flim fit
Support list for users of FLIMfit Fluorescence Lifetime Imaging tool.
flimfit-users at lists.openmicroscopy.org.uk
Wed Nov 14 14:41:13 GMT 2018
Hi,
I have built a time gated Raman instrument that is capable of acquiring FLIM data.
I was hoping to use FLIMfit to produce the lifetime images but I am having difficulty determining the correct format for my data.
I have tried converting SDT files to ASCii using B&H SPC software but FLIMfit doesn't recongize the format once converted to ASCii.
I have tried producing stacks of images each image with a a different time stamp - however I don't think they are labelled correctly.
If someone could provide me with a reference data set in ascii set or a template for my stacked images that would be greatly appreciated!
Sincerely,
Christopher Corden.
University of Nottingham,
Biophotonics group.
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