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<p style="margin-top:0;margin-bottom:0">Hello David,</p>
<p style="margin-top:0;margin-bottom:0">Thanks for your reply. Since . <span style="color: rgb(33, 33, 33); font-family: wf_segoe-ui_normal, "Segoe UI", "Segoe WP", Tahoma, Arial, sans-serif, serif, EmojiFont; font-size: 15px;"> ‘</span><span style="font-size: 15px; color: rgb(69, 69, 69); font-family: "Helvetica Neue", serif, EmojiFont;">%s</span><span style="color: rgb(33, 33, 33); font-family: wf_segoe-ui_normal, "Segoe UI", "Segoe WP", Tahoma, Arial, sans-serif, serif, EmojiFont; font-size: 15px;">’
in the conversion will split the output into a separate file for each series, and i want a single file, I used the below command.</span></p>
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<p style="margin-top:0;margin-bottom:0"><span style="color: rgb(33, 33, 33); font-family: wf_segoe-ui_normal, "Segoe UI", "Segoe WP", Tahoma, Arial, sans-serif, serif, EmojiFont; font-size: 15px;"></p>
<pre style="overflow: auto hidden; padding: 5px; background-color: rgb(236, 239, 241); color: rgb(69, 90, 100); line-height: 15.6px; border-top: 1px solid rgb(170, 204, 153); border-bottom: 1px solid rgb(170, 204, 153); border-image: initial; border-left: none; border-right: none;">bfconvert /path/to/input output.tiff</pre>
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<p style="margin-top:0;margin-bottom:0"> The problem is the generated file is corrupted and i am unable to view it in the viewer properly. I was able to view the scn file before conversion but the tiff file generated was not the same.</p>
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<p style="margin-top:0;margin-bottom:0">Please resolve this issue. I uploaded my .scn file in the given link. Please let me know if you can properly convert it in to the tiff file.</p>
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<p style="margin-top:0;margin-bottom:0">Thanks,</p>
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<p>Mohanapriya</p>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> ome-users <ome-users-bounces@lists.openmicroscopy.org.uk> on behalf of David Gault (Staff) <d.gault@dundee.ac.uk><br>
<b>Sent:</b> Thursday, March 7, 2019 9:43:38 AM<br>
<b>To:</b> OME User Support List<br>
<b>Cc:</b> ome-users-request@lists.openmicroscopy.org.uk; devel OME; ome-users-bounces@lists.openmicroscopy.org.uk<br>
<b>Subject:</b> Re: [ome-users] SCN TO TIFF CONVERSION</font>
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<div class="" style="word-wrap:break-word">Hi Mohanapriya,
<div class=""><br class="">
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<div class="">Using ‘<span class="" style="color:rgb(69,69,69); font-family:'Helvetica Neue',serif,EmojiFont">%s</span>’ in the conversion will split the output into a separate file for each series. Is that what you intended? When you say that the planes are
separate is it the channel fields that are separate but you wish them to be interleaved? The bfconvert command currently bases the channel interleaving of the output file as the same as the that used for the input reader. </div>
<div class=""><br class="">
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<div class="">I was unable to access the file you linked, would you be able to upload it to <a href="https://www.openmicroscopy.org/qa2/qa/upload/?" class="">https://www.openmicroscopy.org/qa2/qa/upload/?</a></div>
<div class=""><br class="">
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<div class="">With Thanks,</div>
<div class="">David Gault</div>
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<blockquote type="cite" class="">
<div class="">On 6 Mar 2019, at 19:36, Narapareddy, Mohanapriya <<a href="mailto:mohanapriya.narapareddy@emory.edu" class="">mohanapriya.narapareddy@emory.edu</a>> wrote:</div>
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Narapareddy, Mohanapriya has shared a OneDrive for Business file with you. To view it, click the link below.</div>
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<td class=""><a href="https://emory-my.sharepoint.com/personal/mnarapa_emory_edu/Documents/Attachments/C3L.01064.21.scn" target="_blank" class="">
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<div class="" style="margin-top:0px; margin-bottom:0px">Hello,</div>
<div class="" style="margin-top:0px; margin-bottom:0px">I am facing problem with scn to tiff conversion. </div>
<div class="" style="margin-top:0px; margin-bottom:0px">Please help me with this issue.</div>
<div class="" style="margin-top:0px; margin-bottom:0px">Here i attach my scn file.</div>
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<div class="" style="margin-top:0px; margin-bottom:0px">Here is the command i used. please correct me if i am wrong.</div>
<div class="" style="margin-top:0px; margin-bottom:0px"><span class="" style="color:rgb(69,69,69); font-family:'Helvetica Neue',serif,EmojiFont; font-size:12px"> </span><span class="" style="color:rgb(69,69,69); font-family:'Helvetica Neue',serif,EmojiFont; font-size:12px">bfconvert
-bigtiff C3L.01064.21.scn sample1-%s.tif</span><br class="">
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<div class="" style="margin-top:0px; margin-bottom:0px">Thanks,</div>
<div class="" style="margin-top:0px; margin-bottom:0px">Mohanapriya</div>
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