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<p class="MsoNormal">Hello,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">we have .FLI files from Lambert Instruments' Capture software (version 1.0.4.0) for the high-speed camera HiCAM FLUO. Images are streamed at high speed and stored as UINT12 (2 pixels in 3 bytes) or optionally as UINT8. The FLIM reader in
bio-formats (<a href="https://github.com/openmicroscopy/bioformats/blob/develop/components/formats-gpl/src/loci/formats/in/LiFlimReader.java">https://github.com/openmicroscopy/bioformats/blob/develop/components/formats-gpl/src/loci/formats/in/LiFlimReader.java</a>)
does not seem to support 12-bit intensities. Also, when asking bio-formats to return the metadata, the whole file is returned (which suggests that the metadata of standard FLIM .FLI files is organized -- or terminated -- differently).
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">The file is binary with the first bytes containing metadata information in readable text format. One field is 'hasDarkImage'. If hasDarkImage == 1, the very last frame is a dark image that is subtracted on-the-fly in the software when displaying
the frames (but the images are stored raw in the file). The 'exposureTime' is in micro seconds. Finally, the software seems to support 'compression', although there is no setting for turning it on in the software. The software supports binning.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Would you be interested in adding support for these files to bio-formats? We have example files that we could upload.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks a lot,<o:p></o:p></p>
<p class="MsoNormal">a2<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">----<o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">Dr. Aaron Ponti<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Software and Data Management Engineer<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Image Analysis Specialist<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Single Cell Facility<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Department of Biosystems Science and Engineering (D-BSSE)<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE-CH" style="color:#1F497D">ETH Zürich<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE-CH" style="color:#1F497D">Office 2.30<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE-CH" style="color:#1F497D">Mattenstrasse 26<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE-CH" style="color:#1F497D">4058 Basel, Switzerland<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE-CH" style="color:#1F497D"><a href="mailto:aaron.ponti@bsse.ethz.ch"><span style="color:#0563C1">aaron.ponti@bsse.ethz.ch</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE-CH" style="color:#1F497D">T: +41 61 387 33 74<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE-CH" style="color:#1F497D">F: +41 61 387 39 93<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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