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<p style="margin-top:0;margin-bottom:0"><span style="color: rgb(0, 0, 0);"> Hello Team,</span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-size: 11pt; font-family: "Times New Roman", Times, serif; color: rgb(0, 0, 0);"><br>
</span></p>
<p style="margin-top:0;margin-bottom:0"><span style="color: rgb(0, 0, 0);"></span><span style="font-size: 11pt; font-family: "Times New Roman", Times, serif; color: rgb(0, 0, 0);">I have been trying Bio-Formats to convert my image which is in scn format </span><span style="font-size: 11pt; font-family: "Times New Roman", Times, serif; color: rgb(0, 0, 0);">(Leica
scn) using the command line bftools.zip. I have received Converted TIFF</span><span style="font-size: 11pt; font-family: "Times New Roman", Times, serif; color: rgb(0, 0, 0);"> files which are huge in size (6GB and 4 GB respectively) </span><span style="font-size: 11pt; font-family: "Times New Roman", Times, serif; color: rgb(0, 0, 0);">as
compared to the original .scn file (300 MB</span><span style="font-size: 11pt; font-family: "Times New Roman", Times, serif; color: rgb(0, 0, 0);"> ) . The images do not open at all in any specialized <span style="font-family: "Times New Roman", Times, serif; font-size: 14.6667px;">software. </span></span><span style="font-size: 11pt; font-family: "Times New Roman", Times, serif; color: rgb(0, 0, 0);">Details
of the error are </span><span style="font-size: 11pt; font-family: "Times New Roman", Times, serif; color: rgb(0, 0, 0);">as follows:</span></p>
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<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif;"><span style="font-size: 11pt; font-family: "Times New Roman", Times, serif; color: rgb(0, 0, 0);">bftools
latest version : </span><span style="font-size: 11pt; font-family: "Times New Roman", Times, serif; color: rgb(0, 0, 0);">A bundle o</span></span><span style="font-family: "Times New Roman", Times, serif; font-size: 11pt; color: rgb(0, 0, 0);"></span><span style="font-family: "Times New Roman", Times, serif; font-size: 11pt; color: rgb(0, 0, 0);">f
scripts for using Bio-Formats on the command line with bioformats_package.jar already included. Windows Operating system with 4 GB RAM. I have included in the attachment all the information.</span></span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif;"><br>
</span></span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif; font-size: 11pt; color: rgb(0, 0, 0);">I
am unable to open the converted image in any other software as well. </span></span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif;"><br>
</span></span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif;"><span style="font-size: 11pt; font-family: "Times New Roman", Times, serif; color: rgb(0, 0, 0);">Details
like </span><span style="color: rgb(0, 0, 0); font-family: "Times New Roman", Times, serif; font-size: 11pt; text-align: justify;">width, height, number of channels, Z slices, and timepoints are attached in the screenshots.</span></span></span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif;"><span style="color: rgb(0, 0, 0); font-family: "Times New Roman", Times, serif; font-size: 11pt; text-align: justify;"><br>
</span></span></span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif;"><span style="color: rgb(0, 0, 0); font-family: "Times New Roman", Times, serif; font-size: 11pt; text-align: justify;">Please
look into it and advise accordingly.</span></span></span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif;"><span style="color: rgb(0, 0, 0); font-family: "Times New Roman", Times, serif; font-size: 11pt; text-align: justify;"><br>
</span></span></span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif;"><span style="color: rgb(0, 0, 0); font-family: "Times New Roman", Times, serif; font-size: 11pt; text-align: justify;">Warm
Regards,</span></span></span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif;"><span style="color: rgb(0, 0, 0); font-family: "Times New Roman", Times, serif; font-size: 11pt; text-align: justify;">Hitesha</span></span></span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif;"><br>
</span></span></p>
<p style="margin-top:0;margin-bottom:0"><span style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(254, 254, 254); font-size: 11pt; text-align: center;"><span style="font-family: "Times New Roman", Times, serif;"><br>
</span></span></p>
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