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Hi Kai,
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<div class="">Thank you for raising this issue, using the process you followed should be expected to work and does appear to be fine when exporting to other formats such as TIFF. </div>
<div class="">Unfortunately it looks like this is a bug in the ICSWriter which is not saving the custom LUT values during the export. I have opened a Bio-Formats Trello card for this bug at <a href="https://trello.com/c/LzWW3y7F/291-icswriter-not-preserving-luts" class="">https://trello.com/c/LzWW3y7F/291-icswriter-not-preserving-luts</a> </div>
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<div class="">With Thanks,</div>
<div class="">David Gault</div>
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<div class="">On 2 Nov 2018, at 17:47, Kai Schleicher <<a href="mailto:kai.schleicher@unibas.ch" class="">kai.schleicher@unibas.ch</a>> wrote:</div>
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<div class="">Dear OME team,<br class="">
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I wish to apply a custom LUT in ImageJ and then save the image as ICS-1 using Bio-formats.<br class="">
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When I import the image again with Bio-formats, it does not show the LUT (see imageJ macro in below quote to reproduce the steps).<br class="">
<br class="">
Did I miss an option during import, or is there maybe a different way the LUT should be applied to the image for the exporter to save it?<br class="">
<br class="">
Thanks and cheers from Basel,<br class="">
Kai<br class="">
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<blockquote type="cite" class="">run("M51 Galaxy (177K, 16-bits)");<br class="">
run("cool", "display=m51.tif view=net.imagej.display.DefaultDatasetView@7a6b36cd");<br class="">
run("Bio-Formats Exporter", "save=/home/kai/Desktop/m51_cool.ids");<br class="">
run("Bio-Formats Importer", "open=/home/kai/Desktop/m51_cool.ids autoscale color_mode=Default rois_import=[ROI manager] view=Hyperstack stack_order=XYCZT");<br class="">
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-- <br class="">
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<blockquote type="cite" class="">Please note my NEW PHONE NUMBERS: +41 61 207 57 31 (direct) +41 61 207 22 50 (central)<<<br class="">
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Kai Schleicher, PhD | Research Associate in Advanced Light Microscopy | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel |<br class="">
Phone: +41 61 207 57 31 (direct) +41 61 207 22 50 (central) | <a href="mailto:kai.schleicher@unibas.ch" class="">
kai.schleicher@unibas.ch</a> | <a href="http://www.biozentrum.unibas.ch" class="">
www.biozentrum.unibas.ch</a> | <a href="http://www.microscopynetwork.unibas.ch" class="">
www.microscopynetwork.unibas.ch</a><br class="">
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