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<p class="MsoNormal">In converting large .svs or .vsi images, I get the following error:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">/home/ltarbox/CIR-scans<o:p></o:p></p>
<p class="MsoNormal">54680.svs<o:p></o:p></p>
<p class="MsoNormal">SVSReader initializing 54680.svs<o:p></o:p></p>
<p class="MsoNormal">Reading IFDs<o:p></o:p></p>
<p class="MsoNormal">Populating metadata<o:p></o:p></p>
<p class="MsoNormal">Populating OME metadata<o:p></o:p></p>
<p class="MsoNormal">[Aperio SVS] -> /home/ltarbox/CIR-scans/out/WSI-2_S%s.ome.tif [OME-TIFF]<o:p></o:p></p>
<p class="MsoNormal">Switching to BigTIFF (by file size)<o:p></o:p></p>
<p class="MsoNormal">Tile size = 256 x 256<o:p></o:p></p>
<p class="MsoNormal">Exception in thread "main" java.lang.IllegalArgumentException<o:p></o:p></p>
<p class="MsoNormal"> at java.nio.Buffer.limit(Buffer.java:275)<o:p></o:p></p>
<p class="MsoNormal"> at loci.common.NIOFileHandle.buffer(NIOFileHandle.java:636)<o:p></o:p></p>
<p class="MsoNormal"> at loci.common.NIOFileHandle.writeSetup(NIOFileHandle.java:642)<o:p></o:p></p>
<p class="MsoNormal"> at loci.common.NIOFileHandle.write(NIOFileHandle.java:505)<o:p></o:p></p>
<p class="MsoNormal"> at loci.common.NIOFileHandle.write(NIOFileHandle.java:499)<o:p></o:p></p>
<p class="MsoNormal"> at loci.common.NIOFileHandle.write(NIOFileHandle.java:487)<o:p></o:p></p>
<p class="MsoNormal"> at loci.common.RandomAccessOutputStream.write(RandomAccessOutputStream.java:217)<o:p></o:p></p>
<p class="MsoNormal"> at loci.formats.tiff.TiffSaver.writeIFDStrips(TiffSaver.java:987)<o:p></o:p></p>
<p class="MsoNormal"> at loci.formats.tiff.TiffSaver.writeImageIFD(TiffSaver.java:451)<o:p></o:p></p>
<p class="MsoNormal"> at loci.formats.tiff.TiffSaver.writeImage(TiffSaver.java:401)<o:p></o:p></p>
<p class="MsoNormal"> at loci.formats.tiff.TiffSaver.writeImage(TiffSaver.java:277)<o:p></o:p></p>
<p class="MsoNormal"> at loci.formats.out.TiffWriter.saveBytes(TiffWriter.java:275)<o:p></o:p></p>
<p class="MsoNormal"> at loci.formats.out.OMETiffWriter.saveBytes(OMETiffWriter.java:218)<o:p></o:p></p>
<p class="MsoNormal"> at loci.formats.tools.ImageConverter.convertTilePlane(ImageConverter.java:748)<o:p></o:p></p>
<p class="MsoNormal"> at loci.formats.tools.ImageConverter.convertPlane(ImageConverter.java:634)<o:p></o:p></p>
<p class="MsoNormal"> at loci.formats.tools.ImageConverter.testConvert(ImageConverter.java:563)<o:p></o:p></p>
<p class="MsoNormal"> at loci.formats.tools.ImageConverter.main(ImageConverter.java:884)<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Smaller images convert fine. <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I tripled the memory size (to 40 GB), but it stll fails<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Ideas?<o:p></o:p></p>
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<p class="MsoNormal" style="border:none;padding:0in"><span style="color:#4472C4"><o:p> </o:p></span></p>
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<p class="MsoNormal"><i><span style="color:#4472C4">Lawrence Tarbox, Ph.D., Dept. of Biomedical Informatics, Univ. of Arkansas for Medical Sciences<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span style="color:#4472C4"><a href="mailto:LTarbox@uams.edu">mailto:LTarbox@uams.edu</a> +1.314.681-2752<o:p></o:p></span></i></p>
<p class="MsoNormal"><o:p> </o:p></p>
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