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Hi Katharine,
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<div class="">Do the dimensions and metadata on the file look to be correct? If you have a sample file which we can test you can upload it to <a href="https://www.openmicroscopy.org/qa2/qa/upload/" class="">https://www.openmicroscopy.org/qa2/qa/upload/</a></div>
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<div class="">With Thanks,</div>
<div class="">David Gault</div>
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<div class="">On 20 Sep 2018, at 17:22, Katharine Hanlon <<a href="mailto:katharine.hanlon@irmc.cc" class="">katharine.hanlon@irmc.cc</a>> wrote:</div>
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<div style="margin-top: 0px; margin-bottom: 0px;" class="">Hello, </div>
<div style="margin-top: 0px; margin-bottom: 0px;" class="">I am trying to open a nikon raw NEF file using bioformats in Fiji. Image appears as black. Have tried checking scaling and unchecking scaling box. Have tried auto contrast and other solutions. Image
always opens black. Is this a bug? Does xml data need to be embedded into NEF or not? </div>
<div style="margin-top: 0px; margin-bottom: 0px;" class="">I am wanting to do spectral separation of image so need to keep NEF format if possible. </div>
<div style="margin-top: 0px; margin-bottom: 0px;" class="">Please advise!</div>
<div style="margin-top: 0px; margin-bottom: 0px;" class="">Thanks</div>
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<div style="margin-top: 0px; margin-bottom: 0px;" class=""><span style="font-family: 'Lucida Sans Unicode', 'Lucida Grande', sans-serif;" class=""><i class=""><span style="background-color: rgb(255, 255, 255);" class=""></span><span style="background-color: rgb(255, 255, 255); color: rgb(0, 111, 201);" class=""><b class=""><span style="color: rgb(0, 111, 201);" class="">Katharine
Hanlon<span class="Apple-converted-space"> </span></span><span style="color: rgb(0, 111, 201);" class="">\</span><span style="color: rgb(0, 111, 201);" class=""> </span><span style="" class=""><span style="" class=""></span><b style="font-family: Calibri, Helvetica, sans-serif; font-size: 16px;" class=""><span style="color: rgb(0, 111, 201); font-size: 12pt;" class="">Medical
Imaging Specialist</span></b></span></b></span></i></span></div>
<span style="color: rgb(0, 111, 201);" class=""></span>
<div style="margin-top: 0px; margin-bottom: 0px;" class=""><span style="font-size: 10pt; font-family: 'Lucida Sans Unicode', 'Lucida Grande', sans-serif;" class=""><b class=""><span style="font-size: 11pt;" class=""><span style="font-size: 9pt; font-family: 'Arial Black', Arial, sans-serif; color: rgb(0, 111, 201);" class="">Scully-Welsh
Cancer Center</span><span style="font-family: 'Arial Black', Arial, sans-serif; font-size: 9pt; color: rgb(0, 111, 201);" class=""><span class="Apple-converted-space"> </span>| </span></span></b></span><span style="font-size: 10pt; font-family: 'Lucida Sans Unicode', 'Lucida Grande', sans-serif;" class=""><b class=""><span style="font-family: 'Arial Black', Arial, sans-serif; font-size: 9pt; color: rgb(0, 111, 201);" class="">Indian
River Medical Center</span></b></span></div>
<div style="margin-top: 0px; margin-bottom: 0px;" class=""><span style="font-size: 10pt; font-family: 'Lucida Sans Unicode', 'Lucida Grande', sans-serif;" class=""><b class=""><span style="font-family: 'Arial Black', Arial, sans-serif; font-size: 9pt;" class=""></span></b></span><b style="color: rgb(33, 33, 33); font-family: Calibri, sans-serif; font-size: 11pt;" class=""><span style="font-size: 12pt; font-family: 'Book Antiqua', serif, serif, EmojiFont; color: rgb(31, 73, 125);" class=""><span class="ms-font-color-themePrimary contextualExtensionHighlight ident_1281_1318 ms-border-color-themePrimary" tabindex="0" style="font-family: 'Arial Black', Arial, sans-serif; font-size: 9pt;">1000
36</span><sup class=""><span class="ms-font-color-themePrimary contextualExtensionHighlight ident_1281_1318 ms-border-color-themePrimary" tabindex="0" style="font-family: 'Arial Black', Arial, sans-serif; font-size: 9pt;">th</span></sup><span class="ms-font-color-themePrimary contextualExtensionHighlight ident_1281_1318 ms-border-color-themePrimary" tabindex="0" style="font-family: 'Arial Black', Arial, sans-serif; font-size: 9pt;"> Street</span></span></b></div>
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<span style="font-size: 9pt;" class=""></span><b class=""><span style="font-size: 12pt; font-family: 'Book Antiqua', serif, serif, EmojiFont; color: rgb(31, 73, 125);" class=""><span class="ms-font-color-themePrimary contextualExtensionHighlight ident_1281_1318 ms-border-color-themePrimary" tabindex="0" style="font-family: 'Arial Black', Arial, sans-serif; font-size: 9pt;">Vero
Beach, FL 32960</span></span></b></div>
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<span style="font-size: 9pt;" class=""></span><b class=""><span style="font-size: 12pt; font-family: 'Book Antiqua', serif, serif, EmojiFont; color: rgb(31, 73, 125);" class=""><span style="font-family: 'Arial Black', Arial, sans-serif; font-size: 9pt;" class="">Tel:
772-</span><span style="font-family: 'Arial Black', Arial, sans-serif; font-size: 9pt;" class="">226-4916</span></span></b><b class=""><span style="font-size: 12pt; font-family: 'Book Antiqua', serif, serif, EmojiFont; color: rgb(31, 73, 125);" class=""></span></b></div>
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<span style="font-size: 9pt;" class=""></span><b class=""><span style="font-size: 12pt; font-family: 'Book Antiqua', serif, serif, EmojiFont; color: rgb(31, 73, 125);" class=""><span style="font-family: 'Arial Black', Arial, sans-serif; font-size: 9pt;" class="">Fax:
772-</span><span style="font-family: 'Arial Black', Arial, sans-serif; font-size: 9pt;" class="">226-4930</span></span></b></div>
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