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<p style="margin-top:0;margin-bottom:0">Hi David, </p>
<p style="margin-top:0;margin-bottom:0">Thank you for your help!</p>
<p style="margin-top:0;margin-bottom:0"><br>
</p>
<p style="margin-top:0;margin-bottom:0">I uploaded a sample file. The dimensions and size appear to be correct when opened; however there is no color information- the "value=0" when hovering anywhere in the black box. the sample file is MCF_1934.</p>
<p style="margin-top:0;margin-bottom:0">Thanks again!</p>
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<p style="margin-top:0; margin-bottom:0"><span style="font-family:"Lucida Sans Unicode","Lucida Grande",sans-serif"><i><span style="background-color:rgb(255,255,255)"></span><span style="background-color:rgb(255,255,255); color:rgb(0,111,201)"><b><span style="color:rgb(0,111,201)">Katharine
Hanlon </span><span style="color:rgb(0,111,201)">\</span><span style="color:rgb(0,111,201)"> </span><span style="color:rgb(0,0,0)"><span style="color:rgb(0,0,0)"></span><b style="font-family:Calibri,Helvetica,sans-serif; font-size:16px"><span style="color:rgb(0,111,201); font-size:12pt">Medical
Imaging Specialist</span></b></span></b></span></i></span></p>
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<p style="margin-top:0; margin-bottom:0"><span style="font-size:10pt; font-family:"Lucida Sans Unicode","Lucida Grande",sans-serif"><b><span style="font-size:11pt"><span style="font-size:9pt; font-family:"Arial Black",Arial,sans-serif; color:rgb(0,111,201)">Scully-Welsh
Cancer Center</span><span style="font-family:"Arial Black",Arial,sans-serif; font-size:9pt; color:rgb(0,111,201)"> | </span></span></b></span><span style="font-size:10pt; font-family:"Lucida Sans Unicode","Lucida Grande",sans-serif"><b><span style="font-family:"Arial Black",Arial,sans-serif; font-size:9pt; color:rgb(0,111,201)">Indian
River Medical Center</span></b></span></p>
<p style="margin-top:0; margin-bottom:0"><span style="font-size:10pt; font-family:"Lucida Sans Unicode","Lucida Grande",sans-serif"><b><span style="font-family:"Arial Black",Arial,sans-serif; font-size:9pt"></span></b></span><b style="color:rgb(33,33,33); font-family:Calibri,sans-serif; font-size:11pt"><span style="font-size:12pt; font-family:"Book Antiqua",serif,serif,EmojiFont; color:rgb(31,73,125)"><span class="contextualExtensionHighlight ms-font-color-themePrimary ms-border-color-themePrimary ident_1281_1318" tabindex="0" style="font-family:"Arial Black",Arial,sans-serif; font-size:9pt">1000
36</span><sup><span class="contextualExtensionHighlight ms-font-color-themePrimary ms-border-color-themePrimary ident_1281_1318" tabindex="0" style="font-family:"Arial Black",Arial,sans-serif; font-size:9pt">th</span></sup><span class="contextualExtensionHighlight ms-font-color-themePrimary ms-border-color-themePrimary ident_1281_1318" tabindex="0" style="font-family:"Arial Black",Arial,sans-serif; font-size:9pt"> Street</span></span></b></p>
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Beach, FL 32960</span></span></b></p>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> ome-users <ome-users-bounces@lists.openmicroscopy.org.uk> on behalf of David Gault (Staff) <d.gault@dundee.ac.uk><br>
<b>Sent:</b> Friday, September 21, 2018 6:04:36 AM<br>
<b>To:</b> OME User Support List<br>
<b>Subject:</b> EXTERNAL: Re: [ome-users] help</font>
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<p style="color:red">ATTENTION: This email was sent from someone outside of Indian River Medical Center. Always use caution when opening attachments or clicking links from unknown senders or when receiving unexpected emails.
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<div>Hi Katharine,
<div class=""><br class="">
</div>
<div class="">Do the dimensions and metadata on the file look to be correct? If you have a sample file which we can test you can upload it to <a href="https://www.openmicroscopy.org/qa2/qa/upload/" class="">https://www.openmicroscopy.org/qa2/qa/upload/</a></div>
<div class=""><br class="">
</div>
<div class="">With Thanks,</div>
<div class="">David Gault</div>
<div class=""><br class="">
<div>
<blockquote type="cite" class="">
<div class="">On 20 Sep 2018, at 17:22, Katharine Hanlon <<a href="mailto:katharine.hanlon@irmc.cc" class="">katharine.hanlon@irmc.cc</a>> wrote:</div>
<br class="x_Apple-interchange-newline">
<div class="">
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<div class="" style="margin-top:0px; margin-bottom:0px">Hello, </div>
<div class="" style="margin-top:0px; margin-bottom:0px">I am trying to open a nikon raw NEF file using bioformats in Fiji. Image appears as black. Have tried checking scaling and unchecking scaling box. Have tried auto contrast and other solutions. Image always
opens black. Is this a bug? Does xml data need to be embedded into NEF or not? </div>
<div class="" style="margin-top:0px; margin-bottom:0px">I am wanting to do spectral separation of image so need to keep NEF format if possible. </div>
<div class="" style="margin-top:0px; margin-bottom:0px">Please advise!</div>
<div class="" style="margin-top:0px; margin-bottom:0px">Thanks</div>
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<div class="" style="margin-top:0px; margin-bottom:0px"><span class="" style="font-family:'Lucida Sans Unicode','Lucida Grande',sans-serif"><i class=""><span class="" style="background-color:rgb(255,255,255)"></span><span class="" style="background-color:rgb(255,255,255); color:rgb(0,111,201)"><b class=""><span class="" style="color:rgb(0,111,201)">Katharine
Hanlon<span class="x_Apple-converted-space"> </span></span><span class="" style="color:rgb(0,111,201)">\</span><span class="" style="color:rgb(0,111,201)"> </span><span class="" style=""><span class="" style=""></span><b class="" style="font-family:Calibri,Helvetica,sans-serif; font-size:16px"><span class="" style="color:rgb(0,111,201); font-size:12pt">Medical
Imaging Specialist</span></b></span></b></span></i></span></div>
<span class="" style="color:rgb(0,111,201)"></span>
<div class="" style="margin-top:0px; margin-bottom:0px"><span class="" style="font-size:10pt; font-family:'Lucida Sans Unicode','Lucida Grande',sans-serif"><b class=""><span class="" style="font-size:11pt"><span class="" style="font-size:9pt; font-family:'Arial Black',Arial,sans-serif; color:rgb(0,111,201)">Scully-Welsh
Cancer Center</span><span class="" style="font-family:'Arial Black',Arial,sans-serif; font-size:9pt; color:rgb(0,111,201)"><span class="x_Apple-converted-space"> </span>| </span></span></b></span><span class="" style="font-size:10pt; font-family:'Lucida Sans Unicode','Lucida Grande',sans-serif"><b class=""><span class="" style="font-family:'Arial Black',Arial,sans-serif; font-size:9pt; color:rgb(0,111,201)">Indian
River Medical Center</span></b></span></div>
<div class="" style="margin-top:0px; margin-bottom:0px"><span class="" style="font-size:10pt; font-family:'Lucida Sans Unicode','Lucida Grande',sans-serif"><b class=""><span class="" style="font-family:'Arial Black',Arial,sans-serif; font-size:9pt"></span></b></span><b class="" style="color:rgb(33,33,33); font-family:Calibri,sans-serif; font-size:11pt"><span class="" style="font-size:12pt; font-family:'Book Antiqua',serif,serif,EmojiFont; color:rgb(31,73,125)"><span class="x_ms-font-color-themePrimary x_contextualExtensionHighlight x_ident_1281_1318 x_ms-border-color-themePrimary" tabindex="0" style="font-family:'Arial Black',Arial,sans-serif; font-size:9pt">1000
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<div class="" style="margin:0in 0in 0.0001pt; font-size:11pt; font-family:Calibri,sans-serif; color:rgb(33,33,33)">
<span class="" style="font-size:9pt"></span><b class=""><span class="" style="font-size:12pt; font-family:'Book Antiqua',serif,serif,EmojiFont; color:rgb(31,73,125)"><span class="x_ms-font-color-themePrimary x_contextualExtensionHighlight x_ident_1281_1318 x_ms-border-color-themePrimary" tabindex="0" style="font-family:'Arial Black',Arial,sans-serif; font-size:9pt">Vero
Beach, FL 32960</span></span></b></div>
<span class="" style="font-size:9pt"></span>
<div class="" style="margin:0in 0in 0.0001pt; font-size:11pt; font-family:Calibri,sans-serif; color:rgb(33,33,33)">
<span class="" style="font-size:9pt"></span><b class=""><span class="" style="font-size:12pt; font-family:'Book Antiqua',serif,serif,EmojiFont; color:rgb(31,73,125)"><span class="" style="font-family:'Arial Black',Arial,sans-serif; font-size:9pt">Tel: 772-</span><span class="" style="font-family:'Arial Black',Arial,sans-serif; font-size:9pt">226-4916</span></span></b><b class=""><span class="" style="font-size:12pt; font-family:'Book Antiqua',serif,serif,EmojiFont; color:rgb(31,73,125)"></span></b></div>
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