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<p class="x_MsoNormal"><span style="font-size:11.0pt">Dear all,</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt"> </span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">We are pleased to announce the release of version 5.1.0 of the open-source fluorescence lifetime fitting and visualisation tool FLIMfit, providing a number of new features. Download version 5.1.0 here:
<a href="https://flimfit.org/downloads/5.1.0/" id="LPlnk717229" class="OWAAutoLink" previewremoved="true">
https://flimfit.org/downloads/5.1.0/</a> .</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt"> </span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">Please note that this version of FLIMfit uses Maximum Likelihood fitting by default. This is the best choice for TCSPC datasets.</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt"> </span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">We have also recently released
<i>Galene</i>, a new tool for motion correction of intravital FLIM data. <i>Galene</i> can correct for motion between and within frames of FIFO FLIM data to retrieve clear images from otherwise unusable data. You can download the software at
<a href="https://galene.flimfit.org" id="LPlnk795696" class="OWAAutoLink" previewremoved="true">
https://galene.flimfit.org</a>. The software is described in our recent eLife paper
<a href="https://elifesciences.org/articles/35800" id="LPlnk558111" class="OWAAutoLink" previewremoved="true">
https://elifesciences.org/articles/35800</a> . Any feedback or comments are very welcome!
</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt"> </span></p>
<p class="x_MsoNormal"><b><span style="font-size:11.0pt">New Features in FLIMfit 5.1.0</span></b></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">- New phasor-plot based segmentation allows, for example, gating out of tissue autofluorescence</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">- Load motion-corrected FLIM data from Galene
</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt"> </span></p>
<p class="x_MsoNormal"><b><span style="font-size:11.0pt">Enhancements</span></b></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">- Use Maximum Likelihood fitting by default</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">- Improved IRF estimation using photodiode model</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">- Load compressed B&H FIFO data with updated Bioformats</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">- Improve compatibility with LaVision .ome.tifs</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">- Save colourscale limits in exported image metadata
</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt"> </span></p>
<p class="x_MsoNormal"><b><span style="font-size:11.0pt">Bug fixes</span></b></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">- Fix ability to load plain TIF stacks</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">- Fix regression in reference reconvolution</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt"> </span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt"> </span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">Best regards,</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt">Sean Warren</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt"> </span></p>
<p class="x_MsoNormal"><b><span style="font-size:10.0pt; font-family:Helvetica">Dr. Sean Warren </span></b><span style="font-size:10.0pt; font-family:Helvetica">| Research Officer<br>
Invasion and Metastasis<br>
Cancer Division<br>
<b>Garvan Institute of Medical Research </b><br>
The Kinghorn Cancer Centre,<b> </b>370 Victoria Street, Darlinghurst, NSW 2010</span></p>
<p class="x_MsoNormal"> </p>
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