<div dir="ltr">Hi David,<div><br></div><div>Thank you for your reply. </div><div>
<p class="inbox-inbox-p1"><span class="inbox-inbox-s1">Version: 5.5.2</span></p>
<p class="inbox-inbox-p1"><span class="inbox-inbox-s1">Build date: 15 June 2017</span></p>
<p class="inbox-inbox-p1"><span class="inbox-inbox-s1">VCS revision: 4c2f2b00a12aa8a3894c54459a4cf02d5b02e161</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1">I am using the command:</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1">
</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1">../../bftools/bfconvert S34146_A1-Aromatase_01.vsi output.tiff </span></p><p class="inbox-inbox-inbox-inbox-p1"><span class="inbox-inbox-inbox-inbox-s1">(not anything else) and I am getting the following error:</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1">S34146_A1-Aromatase_01.vsi</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1">CellSensReader initializing S34146_A1-Aromatase_01.vsi</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1">[CellSens VSI] -> output.tiff [Tagged Image File Format]</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1">Switching to BigTIFF (by file size)</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1">Tile size = 512 x 512</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1">Exception in thread "main" java.lang.NullPointerException</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1"><span class="inbox-inbox-Apple-tab-span"> </span>at java.lang.System.arraycopy(Native Method)</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1"><span class="inbox-inbox-Apple-tab-span"> </span>at loci.formats.in.CellSensReader.openBytes(CellSensReader.java:538)</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1"><span class="inbox-inbox-Apple-tab-span"> </span>at loci.formats.FormatReader.openBytes(FormatReader.java:886)</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1"><span class="inbox-inbox-Apple-tab-span"> </span>at loci.formats.ImageReader.openBytes(ImageReader.java:441)</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1"><span class="inbox-inbox-Apple-tab-span"> </span>at loci.formats.tools.ImageConverter.convertTilePlane(ImageConverter.java:698)</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1"><span class="inbox-inbox-Apple-tab-span"> </span>at loci.formats.tools.ImageConverter.convertPlane(ImageConverter.java:634)</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1"><span class="inbox-inbox-Apple-tab-span"> </span>at loci.formats.tools.ImageConverter.testConvert(ImageConverter.java:563)</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1">
</span></p><p class="inbox-inbox-p1"><span class="inbox-inbox-s1"><span class="inbox-inbox-Apple-tab-span"> </span>at loci.formats.tools.ImageConverter.main(ImageConverter.java:884)</span></p><p class="inbox-inbox-p1">I have no clue howo should I fix this exception. Looking for your help.</p><p class="inbox-inbox-p1">Thank you,</p><p class="inbox-inbox-p1">Mousumi.</p></div></div><br><div class="gmail_quote"><div dir="ltr">On Thu, Jun 21, 2018 at 6:18 AM David Gault (Staff) <<a href="mailto:d.gault@dundee.ac.uk">d.gault@dundee.ac.uk</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
<div style="word-wrap:break-word">
Hi Mousumi,
<div><br>
</div>
<div>We received the file you uploaded and I have been able to test it with a number of different Bio-Formats versions. So far I have been unable to reproduce the error you were seeing using the command line tools. Do you know which version of Bio-Formats
you are currently using (you can find out by using <span style="font-family:Menlo;font-size:11px">bfconvert -version)</span>? Are you using any other options or parameters when performing the conversion?</div>
<div><br>
</div>
<div>With Thanks,</div>
<div>David Gault</div></div><div style="word-wrap:break-word">
<div><br>
<div>
<blockquote type="cite">
<div>On 20 Jun 2018, at 18:18, Mousumi Roy <<a href="mailto:mousumi.roy47@gmail.com" target="_blank">mousumi.roy47@gmail.com</a>> wrote:</div>
<br class="m_8850326165244786219Apple-interchange-newline">
<div>
<div dir="ltr">Hello David,
<div><br>
</div>
<div>I have uploaded the .vsi and supporting files in the link. Kindly let me know if it is accessible properly. Also, I have a request to not to share this image with others.</div>
<div><br>
</div>
<div>Thank you,</div>
<div>Mousumi.</div>
<div><br>
</div>
<div><br>
</div>
</div>
<br>
<div class="gmail_quote">
<div dir="ltr">On Wed, Jun 20, 2018 at 10:04 AM David Gault (Staff) <<a href="mailto:d.gault@dundee.ac.uk" target="_blank">d.gault@dundee.ac.uk</a>> wrote:<br>
</div>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
<div style="word-wrap:break-word">Hi Mousumi,
<div><br>
</div>
<div>Thank you for reporting the issue, this is not a bug which we are aware of with VSI, as such it would be good to have a file that reproduces the problem for testing and debugging. If you have a sample file which you can provide for testing you
can upload it to <a href="https://www.openmicroscopy.org/qa2/qa/upload/" target="_blank">https://www.openmicroscopy.org/qa2/qa/upload/</a></div>
<div><br>
</div>
<div>With Thanks,</div>
<div>David Gault</div>
<div><br>
<div>
<blockquote type="cite">
<div>On 19 Jun 2018, at 17:09, Mousumi Roy <<a href="mailto:mousumi.roy@stonybrook.edu" target="_blank">mousumi.roy@stonybrook.edu</a>> wrote:</div>
<br class="m_8850326165244786219m_8256412102395543556Apple-interchange-newline">
<div>
<div dir="ltr"><br clear="all">
<div>Hello,</div>
<div><br>
</div>
<div>I am using bftools and trying to convert .vsi images into .tiff using
<strong class="m_8850326165244786219m_8256412102395543556gmail-command" style="color:rgb(55,71,79);font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255);text-decoration-style:initial;text-decoration-color:initial">
bfconvert</strong><span style="color:rgb(55,71,79);font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255);text-decoration-style:initial;text-decoration-color:initial;float:none;display:inline"><span> </span></span><span style="text-decoration:none;font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255)"><a class="m_8850326165244786219m_8256412102395543556gmail-internal m_8850326165244786219m_8256412102395543556gmail-reference" href="https://docs.openmicroscopy.org/bio-formats/5.7.1/users/comlinetools/index.html" style="color:rgb(29,141,205);text-decoration:none;font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255)" target="_blank">command
line tool</a><font color="#37474f">. But I am getting following error:</font></span></div>
<div><em style="text-decoration:none;font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255)"><font color="#37474f"><strong style="box-sizing:border-box;font-weight:600;color:rgb(36,41,46);font-family:-apple-system,BlinkMacSystemFont,"Segoe UI",Helvetica,Arial,sans-serif,"Apple Color Emoji","Segoe UI Emoji","Segoe UI Symbol";font-size:14px;font-style:normal;text-align:start;background-color:rgb(255,255,255);text-decoration-style:initial;text-decoration-color:initial">Exception
in thread "main" loci.formats.FormatException: Plane index:1 must be < 1<br style="box-sizing:border-box">
at loci.formats.FormatWriter.checkParams(FormatWriter.java:387)<br style="box-sizing:border-box">
at loci.formats.out.TiffWriter.saveBytes(TiffWriter.java:215)<br style="box-sizing:border-box">
at loci.formats.tools.ImageConverter.convertTilePlane(ImageConverter.java:748)<br style="box-sizing:border-box">
at loci.formats.tools.ImageConverter.convertPlane(ImageConverter.java:634)<br style="box-sizing:border-box">
at loci.formats.tools.ImageConverter.testConvert(ImageConverter.java:563)<br style="box-sizing:border-box">
at loci.formats.tools.ImageConverter.main(ImageConverter.java:884)</strong> <br>
</font></em></div>
<div><em style="text-decoration:none;font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255)"><font color="#37474f"><br>
</font></em></div>
<div style="text-align:justify"><font color="#37474f" face="Open Sans, Helvetica Neue, Helvetica, Arial, sans-serif"><span style="font-size:16px">I have 40x .vsi files which I need to convert into .tiff format but it seems I am not
able to convert without compression. Kindly assist me.</span></font></div>
<div style="text-align:justify"><font color="#37474f" face="Open Sans, Helvetica Neue, Helvetica, Arial, sans-serif"><span style="font-size:16px"><br>
</span></font></div>
<div style="text-align:justify"><font color="#37474f" face="Open Sans, Helvetica Neue, Helvetica, Arial, sans-serif"><span style="font-size:16px">Thank you. Looking forward to your response.</span></font></div>
<div><br>
</div>
Sincerely,<br>
<div dir="ltr" class="m_8850326165244786219m_8256412102395543556gmail_signature" data-smartmail="gmail_signature">
<div dir="ltr">
<table cellpadding="0" cellspacing="0" border="0" style="background:none;border-collapse:collapse;border-spacing:0px;color:rgb(85,85,85);font-family:proxima-nova,"Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;border:0px;margin:0px;padding:0px">
<tbody>
<tr>
<td colspan="2" style="font-family:Times,Georgia,serif;padding-bottom:5px;color:rgb(247,117,31)">
Mousumi Roy</td>
</tr>
<tr>
<td colspan="2" style="font-family:Times,Georgia,serif;color:rgb(51,51,51);font-size:12px">
<i>Graduate Research assistant</i></td>
</tr>
<tr>
<td colspan="2" style="font-family:Times,Georgia,serif;color:rgb(51,51,51);font-size:12px">
Computer Science</td>
</tr>
<tr>
<td colspan="2" style="font-family:Times,Georgia,serif;color:rgb(51,51,51);font-size:12px">
<span style="font-weight:700">Stony Brook University</span></td>
</tr>
<tr>
<td width="20" valign="top" style="font-family:Times,Georgia,serif;vertical-align:top;width:20px;color:rgb(247,117,31);font-size:12px">
m:</td>
<td style="font-family:Times,Georgia,serif;color:rgb(51,51,51);font-size:12px">
<a href="tel:(631)%20627-5860" value="+16316275860" target="_blank">6316275860</a></td>
</tr>
<tr>
<td width="20" valign="top" style="font-family:Times,Georgia,serif;vertical-align:top;width:20px;color:rgb(247,117,31);font-size:12px">
a:</td>
<td valign="top" style="font-family:Times,Georgia,serif;vertical-align:top;color:rgb(51,51,51);font-size:12px">
<a href="https://maps.google.com/?q=100+Nicolls+Rd,+Stony+Brook,+NY+11794&entry=gmail&source=g" target="_blank">100 Nicolls Rd, Stony Brook, NY 11794</a></td>
</tr>
<tr>
<td width="20" valign="top" style="font-family:Times,Georgia,serif;vertical-align:top;width:20px;color:rgb(247,117,31);font-size:12px">
e:</td>
<td valign="top" style="font-family:Times,Georgia,serif;vertical-align:top;color:rgb(51,51,51);font-size:12px">
<a href="mailto:mousumi.roy@stonybrook.edu" style="color:rgb(29,161,219)" target="_blank">mousumi.roy@stonybrook.edu</a></td>
</tr>
</tbody>
</table>
</div>
</div>
</div>
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</blockquote>
</div>
<br>
</div>
<br>
<span style="font-size:10pt">The University of Dundee is a registered Scottish Charity, No: SC015096</span>
</div>
</blockquote>
</div>
</div>
</blockquote>
</div>
<br>
</div>
<br>
<span style="font-size:10pt">The University of Dundee is a registered Scottish Charity, No: SC015096</span>
</div></blockquote></div>