<div dir="ltr"><br clear="all"><div>Hello,</div><div><br></div><div>I am using bftools and trying to convert .vsi images into .tiff using
<strong class="gmail-command" style="color:rgb(55,71,79);font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255);text-decoration-style:initial;text-decoration-color:initial">bfconvert</strong><span style="color:rgb(55,71,79);font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255);text-decoration-style:initial;text-decoration-color:initial;float:none;display:inline"><span> </span></span><span style="text-decoration:none;font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255)"><a class="gmail-reference gmail-internal" href="https://docs.openmicroscopy.org/bio-formats/5.7.1/users/comlinetools/index.html" style="color:rgb(29,141,205);text-decoration:none;font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255)">command line tool</a><font color="#37474f" style="">. But I am getting following error:</font></span></div><div><em style="text-decoration:none;font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255)"><font color="#37474f">
<strong style="box-sizing:border-box;font-weight:600;color:rgb(36,41,46);font-family:-apple-system,BlinkMacSystemFont,"Segoe UI",Helvetica,Arial,sans-serif,"Apple Color Emoji","Segoe UI Emoji","Segoe UI Symbol";font-size:14px;font-style:normal;text-align:start;background-color:rgb(255,255,255);text-decoration-style:initial;text-decoration-color:initial">Exception in thread "main" loci.formats.FormatException: Plane index:1 must be < 1<br style="box-sizing:border-box">at loci.formats.FormatWriter.checkParams(FormatWriter.java:387)<br style="box-sizing:border-box">at loci.formats.out.TiffWriter.saveBytes(TiffWriter.java:215)<br style="box-sizing:border-box">at loci.formats.tools.ImageConverter.convertTilePlane(ImageConverter.java:748)<br style="box-sizing:border-box">at loci.formats.tools.ImageConverter.convertPlane(ImageConverter.java:634)<br style="box-sizing:border-box">at loci.formats.tools.ImageConverter.testConvert(ImageConverter.java:563)<br style="box-sizing:border-box">at loci.formats.tools.ImageConverter.main(ImageConverter.java:884)</strong>
<br></font></em></div><div><em style="text-decoration:none;font-family:"Open Sans","Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;text-align:justify;background-color:rgb(255,255,255)"><font color="#37474f"><br></font></em></div><div style="text-align:justify"><font color="#37474f" face="Open Sans, Helvetica Neue, Helvetica, Arial, sans-serif"><span style="font-size:16px">I have 40x .vsi files which I need to convert into .tiff format but it seems I am not able to convert without compression. Kindly assist me.</span></font></div><div style="text-align:justify"><font color="#37474f" face="Open Sans, Helvetica Neue, Helvetica, Arial, sans-serif"><span style="font-size:16px"><br></span></font></div><div style="text-align:justify"><font color="#37474f" face="Open Sans, Helvetica Neue, Helvetica, Arial, sans-serif"><span style="font-size:16px">Thank you. Looking forward to your response.</span></font></div><div><br></div>Sincerely,<br><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><table cellpadding="0" cellspacing="0" border="0" style="background:none;border-collapse:collapse;border-spacing:0px;color:rgb(85,85,85);font-family:proxima-nova,"Helvetica Neue",Helvetica,Arial,sans-serif;font-size:16px;border:0px;margin:0px;padding:0px"><tbody><tr><td colspan="2" style="font-family:Times,Georgia,serif;padding-bottom:5px;color:rgb(247,117,31)">Mousumi Roy</td></tr><tr><td colspan="2" style="font-family:Times,Georgia,serif;color:rgb(51,51,51);font-size:12px"><i>Graduate Research assistant</i></td></tr><tr><td colspan="2" style="font-family:Times,Georgia,serif;color:rgb(51,51,51);font-size:12px">Computer Science</td></tr><tr><td colspan="2" style="font-family:Times,Georgia,serif;color:rgb(51,51,51);font-size:12px"><span style="font-weight:700">Stony Brook University</span></td></tr><tr><td width="20" valign="top" style="font-family:Times,Georgia,serif;vertical-align:top;width:20px;color:rgb(247,117,31);font-size:12px">m:</td><td style="font-family:Times,Georgia,serif;color:rgb(51,51,51);font-size:12px">6316275860</td></tr><tr><td width="20" valign="top" style="font-family:Times,Georgia,serif;vertical-align:top;width:20px;color:rgb(247,117,31);font-size:12px">a:</td><td valign="top" style="font-family:Times,Georgia,serif;vertical-align:top;color:rgb(51,51,51);font-size:12px">100 Nicolls Rd, Stony Brook, NY 11794</td></tr><tr><td width="20" valign="top" style="font-family:Times,Georgia,serif;vertical-align:top;width:20px;color:rgb(247,117,31);font-size:12px">e:</td><td valign="top" style="font-family:Times,Georgia,serif;vertical-align:top;color:rgb(51,51,51);font-size:12px"><a href="mailto:mousumi.roy@stonybrook.edu" style="color:rgb(29,161,219)" target="_blank">mousumi.roy@stonybrook.edu</a></td></tr></tbody></table></div></div></div>