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Hi Kelli
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<div class="">My initial thoughts on this are that, looking at your first, log scale, image you seem to have a significant background.</div>
<div class="">FLIMfit has 3 options for handling backgrounds :</div>
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<div class="">1) 'Single value' just sets the background used in the fit to a constant set by the user.</div>
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<div class="">2) More realistically ‘image’ allows you to use a background image (just use the same setup w/out a sample)</div>
<div class="">to cater for spatially-varying backgrounds.</div>
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<div class="">3) Lastly you can use a time-varying background. This is to cater for situations where there is background fluorescence.</div>
<div class="">For example from the cover-slip.</div>
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<div class="">I’d suggest trying 1) to work out if this is the problem.</div>
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<div class="">Best Wishes</div>
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<div class="">Ian</div>
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<blockquote type="cite" class="">
<div class="">On 10 Jan 2018, at 20:09, Kelli Fenelon <<a href="mailto:kelli.fenelon@sickkids.ca" class="">kelli.fenelon@sickkids.ca</a>> wrote:</div>
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<div style="margin-top: 0px; margin-bottom: 0px;" class="">Hi again,</div>
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<div style="margin-top: 0px; margin-bottom: 0px;" class="">I've attached screenshots and my best attempt at explaining my thought process about the fitting issue I'm having. Do you think you can help?</div>
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<div style="margin-top: 0px; margin-bottom: 0px;" class="">Thanks again,</div>
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<div style="margin-top: 0px; margin-bottom: 0px;" class="">Kelli</div>
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<div id="divRplyFwdMsg" dir="ltr" class=""><font face="Calibri, sans-serif" style="font-size: 11pt;" class=""><b class="">From:</b><span class="Apple-converted-space"> </span>ome-users <<a href="mailto:ome-users-bounces@lists.openmicroscopy.org.uk" class="">ome-users-bounces@lists.openmicroscopy.org.uk</a>>
on behalf of Munro, Ian <<a href="mailto:i.munro@imperial.ac.uk" class="">i.munro@imperial.ac.uk</a>><br class="">
<b class="">Sent:</b><span class="Apple-converted-space"> </span>January 10, 2018 5:36:05 AM<br class="">
<b class="">To:</b><span class="Apple-converted-space"> </span>OME User Support List<br class="">
<b class="">Subject:</b><span class="Apple-converted-space"> </span>Re: [ome-users] FLIMfit question</font>
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<div class="" style="word-wrap: break-word;">HI Again Kelli
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<div class="">I was just wondering if any of my suggestions solved your problems?</div>
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<div class="">Best Wishes</div>
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<div class="">Ian</div>
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<div class="">On 4 Jan 2018, at 16:30, Munro, Ian <<a href="mailto:i.munro@imperial.ac.uk" class="">i.munro@imperial.ac.uk</a>> wrote:</div>
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<div class="" style="word-wrap: break-word;">Hi Kelli
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<div class="">Sorry I missed this e-mail earlier.</div>
<div class="">You can use the Segmentation Manager’s File menu to save and reload segmentations.</div>
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<div class="">Best Wishes</div>
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<div class="">Ian</div>
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<div class="">On 3 Jan 2018, at 22:21, Kelli Fenelon <<a href="mailto:kelli.fenelon@sickkids.ca" class="">kelli.fenelon@sickkids.ca</a>> wrote:</div>
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<div class="" style="margin-top: 0px; margin-bottom: 0px;">Hi Ian,</div>
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<div class="" style="margin-top: 0px; margin-bottom: 0px;">That link sends me back to a page that gives me this email address again...</div>
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<div class="" style="margin-top: 0px; margin-bottom: 0px;">The problem with saving I'm having is that when save as a 'raw' file, it forgets my segmentation. I guess what I really want to be able to do is save the segmentation.</div>
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<div class="" style="margin-top: 0px; margin-bottom: 0px;">Thanks again,</div>
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<div class="" style="margin-top: 0px; margin-bottom: 0px;">Kelli</div>
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<font face="Calibri, sans-serif" class="" style="font-size: 11pt;"><b class="">From:</b><span class="x_Apple-converted-space"> </span>ome-users <<a href="mailto:ome-users-bounces@lists.openmicroscopy.org.uk" class="">ome-users-bounces@lists.openmicroscopy.org.uk</a>>
on behalf of Munro, Ian <<a href="mailto:i.munro@imperial.ac.uk" class="">i.munro@imperial.ac.uk</a>><br class="">
<b class="">Sent:</b><span class="x_Apple-converted-space"> </span>January 3, 2018 5:01:14 PM<br class="">
<b class="">To:</b><span class="x_Apple-converted-space"> </span>OME User Support List<br class="">
<b class="">Subject:</b><span class="x_Apple-converted-space"> </span>Re: [ome-users] FLIMfit question</font>
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Hi Kelli
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<div class="">Normally the best way to ask questions is via the flimfit-users list <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.openmicroscopy.org.uk_mailman_listinfo_flimfit-2Dusers&d=DwMGaQ&c=Sj806OTFwmuG2UO1EEDr-2uZRzm2EPz39TfVBG2Km-o&r=7dh5bJkL96w3P1ytEeKLlSJh-HDZau-n0oI2N8-ia1I&m=jlhKZYUaGHQqwUmJkM2FHJu-edlR1HkTxm2JZaao9ig&s=Iqu76w8GRZb3mEFBTZxISm8v5AHmHAKEkaWPcpOI2VY&e=" class="">http://lists.openmicroscopy.org.uk/mailman/listinfo/flimfit-users</a></div>
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<div class="">I’m not sure about zooming the segmentation manager.</div>
<div class="">I’ll investigate and get back to you.</div>
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<div class="">I’m afraid I don’t follow exactly what you mean by saving progress could you please clarify?</div>
<div class="">In case it helps, you can save your data as a ‘raw’ file File->Save as Raw Dataset... to allow fast reloading.</div>
<div class="">You can also save the fit results using File->Save ft results table…</div>
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<div class="">Best Wishes</div>
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<div class="">Ian</div>
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<div class="">On 3 Jan 2018, at 19:15, Kelli Fenelon <<a href="mailto:kelli.fenelon@sickkids.ca" class="">kelli.fenelon@sickkids.ca</a>> wrote:</div>
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<div class="" style="margin-top: 0px; margin-bottom: 0px;">Hi,</div>
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</div>
<div class="" style="margin-top: 0px; margin-bottom: 0px;">I'm not sure if this is the proper method of going about asking my question, but it will have to do whilst my forum account awaits approval. </div>
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<div class="" style="margin-top: 0px; margin-bottom: 0px;">Is there any way to zoom in the segmentation manager or otherwise?</div>
<div class="" style="margin-top: 0px; margin-bottom: 0px;"><br class="">
</div>
<div class="" style="margin-top: 0px; margin-bottom: 0px;">Is there any way to save my progress and continue later? (Save fit/half a segmentation/etc)</div>
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<div class="" style="margin-top: 0px; margin-bottom: 0px;">Thanks a billion,</div>
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<div class="" style="margin-top: 0px; margin-bottom: 0px;">Kelli</div>
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mailing list</span><br style="font-family: Helvetica; font-size: 13px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
<a href="mailto:ome-users@lists.openmicroscopy.org.uk" style="font-family: Helvetica; font-size: 13px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">ome-users@lists.openmicroscopy.org.uk</a><br style="font-family: Helvetica; font-size: 13px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
<a href="http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users" style="font-family: Helvetica; font-size: 13px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users</a><br style="font-family: Helvetica; font-size: 13px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
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