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Hi Dotan,
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<div class="">Thank you for providing the sample data set for testing. I was able to test the dataset provided with the latest Bio-Formats release (5.7.2) and when opening either the .nd file or the .tif (while the .nd is present) the PhysicalSize X and Y are
showing as 0.645 microns and the X and Calibration match. Without the accompanying .nd file I see a value of 32.05 microns. </div>
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<div class="">What value of PhysicalSizeX and PhysicalSizeY were you reading when opening the .nd file via the Bio-Formats Importer?</div>
<div class="">Also which operating system are you using and which version of Bio-Formats and FIJI have you installed? </div>
<div class="">(Versions can be found in FIJI under Help > About ImageJ and Help > About Plugins > Bio-Formats Plugins )</div>
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<div class="">With Thanks,</div>
<div class="">David Gault</div>
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<div class="">On 27 Dec 2017, at 14:48, Dotan Kamber <<a href="mailto:Dotan.Kamber@eb1.co.il" class="">Dotan.Kamber@eb1.co.il</a>> wrote:</div>
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Dears,<o:p class=""></o:p></div>
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<o:p class=""> </o:p></div>
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I'm facing an issue with scaling of MetaMorph tif images acquired with VisiView when opening with FIJI.<o:p class=""></o:p></div>
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When separate tif images are saved as part of an "experiment " with accompanying .nd file the images are opened with the correct scaling (nm/pixel) using the Bio-Formats importer when pointing to the tif images but not upon pointing to the .nd file or upon
drag-and-drop to FIJI.<o:p class=""></o:p></div>
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The scaling is also wrong when a single image (without accompanying .nd file) is opened both via drag-and-drop and the Bio-Formats Importer.<o:p class=""></o:p></div>
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the same images are opened by Drag and Drop.<o:p class=""></o:p></div>
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The following files have been uploaded –<o:p class=""></o:p></div>
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Experiment_2_GFP.nd<o:p class=""></o:p></div>
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Experiment_2_GFP_t1.tif<o:p class=""></o:p></div>
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Experiment_2_GFP_t2.tif<o:p class=""></o:p></div>
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Experiment_3_GFP.tif<o:p class=""></o:p></div>
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For all the correct scaling is 0.645 microns per pixel<o:p class=""></o:p></div>
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This value shows up in the parameters XCalibration and YCalibartion.<o:p class=""></o:p></div>
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It seem that the wrong scaling is retrieved from the parameters XResolution and Yresoltion<o:p class=""></o:p></div>
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<o:p class=""> </o:p></div>
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<span style="font-size: 10pt; font-family: Tahoma, sans-serif;" class="">Happy new year!<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Tahoma, sans-serif;" class="">Dotan</span><b class=""><u class=""><span style="font-size: 14pt; color: blue;" class=""><o:p class=""></o:p></span></u></b></div>
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<o:p class=""> </o:p></div>
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