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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Dear all,<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class=""> </span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">We are pleased to announce the release of FLIMfit<i class=""> </i>5.0.3, available for download at <span style="color: rgb(0, 112, 192);" class=""><a href="http://flimfit.org/downloads/latest" style="color: purple;" class=""><span style="color: rgb(0, 112, 192);" class="">http://flimfit.org/downloads/latest</span></a>.</span><o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Please note that we have updated to Matlab Runtime 2016b, Mac users will need to install the new runtime, available from the downloads page<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">and that this version of FLIMfit is compatible with OMERO 5.2 servers.</span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class=""> <o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">A guide to using FLIMfit to analyse multiphoton FLIM data with tutorial videos is available in a recent paper,<span style="color: rgb(0, 112, 192);" class=""><a href="https://doi.org/10.1016/j.ymeth.2017.04.014" style="color: purple;" class=""><span style="color: rgb(0, 112, 192);" class="">Conway,
JRW, Warren, SC & Timpson, P, Methods (2017)</span></a></span><o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class=""> </span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class=""> </span></div>
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<b class=""><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">New features<o:p class=""></o:p></span></b></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">New segmentation
manager with new freehand tool options and improved performance.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Ability
to estimate IRF from a mono-exponential reference using an exponentially modified gaussian model. This can produce a high quality IRF for TCSPC detectors without secondary peaks or significant afterpulsing.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Add support
for Picoquant HydraHarp and TimeHarp data files.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Add support
for data acquired during bidirectional scan.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Where possible
determine repetition rate from data (currently ptu/pt3 files).<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class=""><o:p class=""></o:p></span></div>
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<b class=""><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Performance Enhancements<o:p class=""></o:p></span></b></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Significantly
improved loading times for some data formats.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Added option
to display smoothed data in the decay tab. When off, this significantly reduces the time to switch between datasets.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">This option
does not affect smoothing during data fitting.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Improved
performance selecting/deselecting images to fit.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Update to
Bioformats 5.4.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class=""><o:p class=""></o:p></span></div>
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<b class=""><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Other Enhancements<o:p class=""></o:p></span></b></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Add option
to plot weighted or unweighted values in plotter.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Visual enhacements
to plotter.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Enable plotting
by image number.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Allow setting
default Gate Max value.<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Improved
support for older MacOS X versions (10.9.x).<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class=""><o:p class=""></o:p></span></div>
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<b class=""><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Bug fixes<o:p class=""></o:p></span></b></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Allow t0
shift map estimation from any loaded dataset (rather than first only)<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Symbol;" class="">·<span style="font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> </span></span><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Fix crash
when fitting t0 and offset locally</span></div>
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</span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class=""> </span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Kind Regards,<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Dr Sean Warren, Garvan Institute of Medical Research<br class="">
Dr Ian Munro, Photonics Group, Imperial College, London<o:p class=""></o:p></span></div>
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<span style="font-size: 10pt; font-family: Arial, sans-serif;" class=""> </span></div>
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<b class=""><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">Dr. Sean Warren </span></b><span style="font-size: 10pt; font-family: Arial, sans-serif;" class="">| Research Officer<br class="">
Invasion and Metastasis<br class="">
Cancer Division<br class="">
<b class="">Garvan Institute of Medical Research </b><br class="">
The Kinghorn Cancer Centre,<b class=""> </b>370 Victoria Street, Darlinghurst, NSW 2010</span></div>
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