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<p class="MsoNormal">Dear list,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I have a 60GB .nd2 file generated using NIS runnin a Nikon microscope. It is a multi-point (25 xy positions), multi-channel (4 channels) time lapse (54 time points). When I open it with BioFormats 5.4 as part of Fjii/ImageJ (2.0.0-rc-59/1.51k;
Java 1.8.0_66 on Windows 8.1, 64-bit), it shows all 25 xy positions in the importer window, but the stack that opens for a point is actually not from one position, but each time point is from a different position. I presume that xy is treated as t and vice
versa. <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I have attached a screenshot of the nd2 opened in NIS Elements to illustrate the structure.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I am not sure whether this is an actual bug or user error. If it is the latter, my apologies, but I would still very much appreciate any support.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Many thanks,<o:p></o:p></p>
<p class="MsoNormal">Max<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB"><img width="423" height="423" id="Picture_x0020_2" src="cid:image002.jpg@01D2A7B3.643E1320"></span><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="color:black;mso-fareast-language:EN-GB">--<br>
Maximilian Fries<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black;mso-fareast-language:EN-GB">PhD student<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black;mso-fareast-language:EN-GB"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:black;mso-fareast-language:EN-GB">Venkitaraman Group<br>
</span><span style="mso-fareast-language:EN-GB">MRC Cancer Unit - University of Cambridge<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB">Hutchison/MRC Research Centre<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB">Box 197, Biomedical Campus<br>
Cambridge<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB">United Kingdom<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB">CB2 0XZ<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:black;mso-fareast-language:EN-GB"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:black;mso-fareast-language:EN-GB">E-Mail:
<a href="mailto:mf474@mrc-cu.cam.ac.uk"><span style="color:blue">mf474@mrc-cu.cam.ac.uk</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black;mso-fareast-language:EN-GB">+44 (0) 1223 763376 (office)<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB"><img border="0" width="117" height="65" id="Picture_x0020_22" src="cid:image006.jpg@01D2A7B3.643E1320" alt="SWAN logo.jpg"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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