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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal>Dear All,<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I have a big stream data from the Hamamatsu sCMOS camera. The file size is more than 10GB and the format can be .BTF(big tiff) or the .HIS format (Hamamatsu format). I tried to use the bioformats library (bfopen) to load in the MATLAB or the ImageJ, in both cases, the latest jar files are added to the path of the software. <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>However, for the HIS format, it only read portion of the data. That is probably due to the memory restriction of the bioformats only read 2 GB <o:p></o:p></p><p class=MsoNormal><span style='color:#1F497D'> error(['Image plane too large. Only 2GB of data can be extracted '...<o:p></o:p></span></p><p class=MsoNormal><span style='color:#1F497D'> 'at one time. You can workaround the problem by opening '...<o:p></o:p></span></p><p class=MsoNormal><span style='color:#1F497D'> 'the plane in tiles.']);<o:p></o:p></span></p><p class=MsoNormal>One suggest from the link <a href="http://www.openmicroscopy.org/site/support/bio-formats5.2/about/bug-reporting.html">http://www.openmicroscopy.org/site/support/bio-formats5.2/about/bug-reporting.html</a><o:p></o:p></p><p class=MsoNormal>But I do not find any examples to use it : <span class=pre><span style='font-size:10.5pt;font-family:Consolas;color:#333333;letter-spacing:.1pt;background:white'>openBytes(int,</span></span><span class=apple-converted-space><span style='font-size:10.5pt;font-family:Consolas;color:#333333;letter-spacing:.1pt;background:white'> </span></span><span class=pre><span style='font-size:10.5pt;font-family:Consolas;color:#333333;letter-spacing:.1pt;background:white'>int,int,</span></span><span class=apple-converted-space><span style='font-size:10.5pt;font-family:Consolas;color:#333333;letter-spacing:.1pt;background:white'> </span></span><span class=pre><span style='font-size:10.5pt;font-family:Consolas;color:#333333;letter-spacing:.1pt;background:white'>int,</span></span><span class=apple-converted-space><span style='font-size:10.5pt;font-family:Consolas;color:#333333;letter-spacing:.1pt;background:white'> </span></span><span class=pre><span style='font-size:10.5pt;font-family:Consolas;color:#333333;letter-spacing:.1pt;background:white'>int)</span></span><span class=apple-converted-space><span style='font-size:10.5pt;font-family:"Lucida Sans Unicode",sans-serif;color:black;letter-spacing:-.1pt;background:white'> <o:p></o:p></span></span></p><p class=MsoNormal><span class=apple-converted-space><span style='font-size:10.5pt;font-family:"Lucida Sans Unicode",sans-serif;color:black;letter-spacing:-.1pt;background:white'><o:p> </o:p></span></span></p><p class=MsoNormal><span class=apple-converted-space><span style='font-size:10.5pt;font-family:"Lucida Sans Unicode",sans-serif;color:black;letter-spacing:-.1pt;background:white'>If I use the big tiff format, the bio-formats can read the small dataset, but the larger dataset (>4GB), there is error to ask the Java advanced Imaging, which I have no clue to install or include. <o:p></o:p></span></span></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Error using bfGetReader (line 84)<o:p></o:p></p><p class=MsoNormal>Java exception occurred:<o:p></o:p></p><p class=MsoNormal>loci.formats.MissingLibraryException: Java Advanced Imaging<o:p></o:p></p><p class=MsoNormal>(JAI) is required to read some TIFF files. Please install JAI<o:p></o:p></p><p class=MsoNormal>from <a href="https://jai.dev.java.net/">https://jai.dev.java.net/</a><o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Can someone help me to find a solution. Really thanks a lot.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Best regards,<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Yuanyuan<o:p></o:p></p></div></body></html>