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<p style="margin-bottom: 0cm; line-height: 100%">Hello again,<br>
</p>
<p style="margin-bottom: 0cm; line-height: 100%">Sorry for emailing
you again but I've just got the hint, that I should be more
specific, since there are different DV files. That's why I tried
all different DV data stacks I got so far from a OMX-V3
microsocope. <br>
</p>
<p style="margin-bottom: 0cm; line-height: 100%">Retrieving the
stage position from the meta data in Matlab works very good for
(processed) data stacks using the provided software from
Deltavision (Softworx). So I can get the information from
reconstructed 3D SIM-stacks, image stacks acquired in STORM
mode(which are technically just single widefield images in a time
series) and 3D deconvoluted stacks.<br>
</p>
<p style="margin-bottom: 0cm; line-height: 100%">Unfortunately it
does not work for unprocessed SIM stacks, conventional 3D-stacks
and which is why I am writing for data stacks with several time
points and just one z-position. (experimental setup: 5000 images
(every 10ms one image)). I know that the information of the stage
position is stored in the data stacks since I can get the
information using Softworx.<br>
</p>
<p style="margin-bottom: 0cm; line-height: 100%">Working minimal
code for 3D-Stack (several z-slices)</p>
<p style="margin-bottom: 0cm; line-height: 100%">r=
bfGetReader(fpath);<br>
m = r.getMetadataStore();<br>
m.getPlanePositionZ(0, 0)</p>
<p style="margin-bottom: 0cm; line-height: 100%"> </p>
<p style="margin-bottom: 0cm; line-height: 100%">ans =
ome.units.quantity.Length: value[3612.2], unit[reference frame]
stored as java.lang.Float</p>
<p style="margin-bottom: 0cm; line-height: 100%"> </p>
<p style="margin-bottom: 0cm; line-height: 100%"><br>
For the data stack with several time points, I just got</p>
<p style="margin-bottom: 0cm; line-height: 100%">ans = []</p>
<p style="margin-bottom: 0cm; line-height: 100%">Do I miss
something, or is there any other commando I could use to get this
information from my data stack?</p>
<p style="margin-bottom: 0cm; line-height: 100%">Thank you very
much!</p>
<p style="margin-bottom: 0cm; line-height: 100%">Cheers,</p>
<p style="margin-bottom: 0cm; line-height: 100%">Alex</p>
<br>
<br>
<div class="moz-cite-prefix">On 20/06/16 19:33, Alexander Al Saroori
wrote:<br>
</div>
<blockquote cite="mid:576836F4.5090303@googlemail.com" type="cite">
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<p style="margin-bottom: 0cm; line-height: 100%">Hi together,</p>
<p style="margin-bottom: 0cm; line-height: 100%">I have some
problems to get the camera position stored in the metadata of my
deltavision (DV) files, using Bio-Formats in Matlab. It works
very good for single image stacks (with several z-slices) but
not for data stacks with several timepoints and just one
z-position. (experimental setup: 5000 images (every 10ms one
image)). I know that the information of the camera position is
stored in the time series since I can get the information using
Softworx (provided Deltavision software).</p>
<p style="margin-bottom: 0cm; line-height: 100%">Working minimal
code for 3D-Stack (several z-slices)</p>
<p style="margin-bottom: 0cm; line-height: 100%">r=
bfGetReader(fpath);<br>
m = r.getMetadataStore();<br>
m.getPlanePositionZ(0, 0)</p>
<p style="margin-bottom: 0cm; line-height: 100%"> </p>
<p style="margin-bottom: 0cm; line-height: 100%">ans =
ome.units.quantity.Length: value[3612.2], unit[reference frame]
stored as java.lang.Float</p>
<p style="margin-bottom: 0cm; line-height: 100%"> </p>
<p style="margin-bottom: 0cm; line-height: 100%"><br>
For the data stack with several timepoints, I just got</p>
<p style="margin-bottom: 0cm; line-height: 100%">ans = []</p>
<p style="margin-bottom: 0cm; line-height: 100%">Do I miss
something, or is there any other commando I could use to get
this information from my data stack?</p>
<p style="margin-bottom: 0cm; line-height: 100%">Thank you very
much!</p>
<p style="margin-bottom: 0cm; line-height: 100%">Cheers,</p>
<p style="margin-bottom: 0cm; line-height: 100%">Alex</p>
<p style="margin-bottom: 0cm; line-height: 100%"><br>
</p>
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