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<span id="docs-internal-guid-965982d8-51e6-69ae-562e-dca41d1621c1" class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">Dear
All,</span></div>
<br class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">We
are pleased to announce the release of version 4.11 of the high-speed open-source Fluorescence Lifetime fitting and visualisation tool FLIMfit.</span></div>
<br class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">Updates/Improvements</span></div>
<br class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">*
Compatible with versions 5.2.x of OMERO.</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">*
OMERO interface has been improved to simplify the handling of attached non-image files.</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">*
Improved error handling/reporting has been added.</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">*
Added ability to resample FIFO data spatially/temporally at load time. </span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">*
FLIMfit now prompts the user when a new version is available.</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">*
An “Exit” option has been added to the “File” menu.</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">*
improved handling of tiff/ome-tiff files (Bio-Formats is now used to distinguish the two for greater reliability.)</span></div>
<br class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">Bug
Fixes</span></div>
<br class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">*
Fix for problems loading single pixel, multichannel Becker & Hickl .sdt files (now using Bio-Formats 5.1.9)</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">*
Problems loading Picoquant .bin files that were introduced in Version 4.10.3 have been addressed.</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">*
Attempts to select combinations of multiple multidimensional files that FLIMfit is incapable of loading are now blocked in the User Interface.</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class=""><br class="">
</span></div>
<span id="docs-internal-guid-965982d8-51e6-f821-a89a-4af561cdfa65" class="">
<div style="line-height: 1.656; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">Known
Issues </span></div>
<br class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">Users
of LaVision BioTec’s Imspector software please note: the Bio-Formats library, which FLIMfit uses, supports version 4.0 of this software. It appears that recent changes to LaVision Imspector mean that FLIM files from version 5.0 are not always correctly interpreted
by the current Bio-Formats reader.</span></div>
<div style="line-height: 1.656; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">The
OME team have open tickets to add support for newer versions of this format.</span></div>
<br class="">
<div style="line-height: 1.656; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">In
the meantime our suggested work-around is to use the capability of this software to generate ome-tiffs.</span></div>
<div style="line-height: 1.656; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">However,
there is an issue here:</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">FLIMfit
supports OME-TIFF files which use the Modulo annotation introduced as a standard in the 2011 version of the OME model to store the FLIM time dimension.</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">The
OME-TIFF files from the Imspector system, unfortunately, use an older version of this standard and cannot, therefore be identified via Bio-Formats as FLIM data.</span></div>
<div style="line-height: 1.656; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">A
patch has been added to FLIMfit to attempt to handle these files but please treat with caution.</span></div>
<br class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">Downloads
and documentation for FLIMfit is available at</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><a href="http://flimfit.org/" style="text-decoration:none;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; color: rgb(17, 85, 204); font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; text-decoration: underline; vertical-align: baseline; white-space: pre-wrap;" class="">http://flimfit.org</span></a></div>
<br class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">Help
pages can be found at </span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="text-decoration: underline; font-size: 13.333333333333332px; font-family: Arial; color: rgb(17, 85, 204); font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class=""><a href="http://help.openmicroscopy.org/flimfit.html" class="">http://help.openmicroscopy.org/flimfit.html</a></span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><br class="">
</div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span id="docs-internal-guid-965982d8-51e7-b028-9fca-27e86d6f4362" class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">The
users list for this software can be found at:</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><a href="http://lists.openmicroscopy.org.uk/mailman/listinfo/flimfit-users" style="text-decoration:none;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; color: rgb(17, 85, 204); font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; text-decoration: underline; vertical-align: baseline; white-space: pre-wrap;" class="">http://lists.openmicroscopy.org.uk/mailman/listinfo/flimfit-users</span></a></div>
<br class="">
<br class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">Kind
Regards</span></div>
<br class="">
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">Dr
Ian Munro</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">Photonics
Group</span></div>
<div style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;" class=""><span style="font-size: 13.333333333333332px; font-family: Arial; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; vertical-align: baseline; white-space: pre-wrap;" class="">Imperial
College, London.</span></div>
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</span></div>
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