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    Hi Kai,<br>
    <br>
    As Sebastien mentions, I have the exact same problem. Sebastien
    provided guidance how to write a script to delete the broken pyramid
    files that block new ones from being generated and as soon as my
    current grant is out the door, I'll look at writing such a script.
    But in case you do it sooner than me, please let us all know. No
    doubt there are others with the same issue.<br>
    <br>
    Note that pyramid generation for those very large
    Axioscan/ZEN-generated OME-TIFFs takes a fairly long time, even on a
    powerful server. But I can attest that it does work on 5.2.2.<br>
    <br>
    Cheers,<br>
    - Damir<br>
    <br>
    <div class="moz-cite-prefix">On 3/8/2016 3:00 AM, Sebastien Besson
      (Staff) wrote:<br>
    </div>
    <blockquote
      cite="mid:6EEB05B9-78F9-4083-89DE-DCDB5691B6F8@dundee.ac.uk"
      type="cite">
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      Hi Kai,
      <div class=""><br class="">
      </div>
      <div class="">thanks for reporting this issue. Looking at the
        images, they were imported back into February</div>
      <div class="">using OMERO 5.2.1 / Bio-Formats 5.1.6. The pyramid
        generation was broken with exactly the</div>
      <div class="">same stack trace than the one reported by Damir in a
        forum thread [1] and fixed in Bio-Formats</div>
      <div class="">5.1.8.</div>
      <div class=""><br class="">
      </div>
      <div class="">There are a different answers to your email:</div>
      <div class=""><br class="">
      </div>
      <div class="">- re-importing the same image into an OMERO 5.2.2
        instance e.g. the demo server should work</div>
      <div class="">  out-of-the-box and the pyramid should be properly
        generated</div>
      <div class="">- in a production environment, deleting the stale
        pyramid files using the command suggested</div>
      <div class="">  in the thread linked above should also work
        without forcing a new import</div>
      <div class="">- from our side, we have created a card to improve
        the behavior illustrated by this use case both</div>
      <div class="">  server-side during pyramid generation and
        client-side e.g. via scripts [2]</div>
      <div class=""><br class="">
      </div>
      <div class="">Best,</div>
      <div class="">Sebastien</div>
      <div class=""><br class="">
      </div>
      <div class="">[1] <a moz-do-not-send="true"
href="https://www.openmicroscopy.org/community/viewtopic.php?f=4&t=7907"
          class="">https://www.openmicroscopy.org/community/viewtopic.php?f=4&t=7907</a></div>
      <div class="">[2] <a moz-do-not-send="true"
          href="https://trello.com/c/TM1Si3UN/38-pyramids" class="">https://trello.com/c/TM1Si3UN/38-pyramids</a></div>
      <div class=""><br class="">
      </div>
      <div class="">
        <div>
          <blockquote type="cite" class="">
            <div class="">On 7 Mar 2016, at 14:01, Kai Schleicher <<a
                moz-do-not-send="true"
                href="mailto:kai.schleicher@unibas.ch" class=""><a class="moz-txt-link-abbreviated" href="mailto:kai.schleicher@unibas.ch">kai.schleicher@unibas.ch</a></a>>
              wrote:</div>
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                  <p class="MsoNormal">Hi,<o:p class=""></o:p></p>
                  <p class="MsoNormal"><o:p class=""> </o:p></p>
                  <p class="MsoNormal">I recorded images in the czi
                    format on the Zeiss Axioscan Slidescanner and
                    exported them via Zen Blue to OME-TIFFs.<o:p
                      class=""></o:p></p>
                  <p class="MsoNormal"><o:p class=""> </o:p></p>
                  <p class="MsoNormal">These OME-Tiffs open correctly in
                    FIJI using the latest Bioformats.<o:p class=""></o:p></p>
                  <p class="MsoNormal"><o:p class=""> </o:p></p>
                  <p class="MsoNormal">However, they fail to open after
                    being imported into OMERO.<o:p class=""></o:p></p>
                  <p class="MsoNormal"><o:p class=""> </o:p></p>
                  <p class="MsoNormal">Import to is handled without
                    error but the images cannot be displayed in the
                    viewer. The also do not have a preview, instead the
                    “watch”-icon is shown.<o:p class=""></o:p></p>
                  <p class="MsoNormal"><o:p class=""> </o:p></p>
                  <p class="MsoNormal">I am using the latest version of
                    OMERO (your demo server) and this client:
                    OMERO.insight-5.2.2-ice35-b17-win.<o:p class=""></o:p></p>
                  <p class="MsoNormal"><o:p class=""> </o:p></p>
                  <p class="MsoNormal">The images in question have the
                    ID 203547 and 203548.<o:p class=""></o:p></p>
                  <p class="MsoNormal"><o:p class=""> </o:p></p>
                  <p class="MsoNormal">Thanks for your help and cheers,<o:p
                      class=""></o:p></p>
                  <p class="MsoNormal">Kai<o:p class=""></o:p></p>
                  <p class="MsoNormal"><o:p class=""> </o:p></p>
                  <p class="MsoNormal">--<o:p class=""></o:p></p>
                  <p class="MsoNormal">Kai Schleicher, PhD | Research
                    Associate in Advanced Light Microscopy | Biozentrum,
                    University of Basel | Klingelbergstrasse 50/70 |
                    CH-4056 Basel<o:p class=""></o:p></p>
                  <p class="MsoNormal">Phone: +41 61 267 22 50 | <a
                      moz-do-not-send="true"
                      href="mailto:kai.schleicher@unibas.ch" class="">
                      <span style="color:#0563C1" class=""><a class="moz-txt-link-abbreviated" href="mailto:kai.schleicher@unibas.ch">kai.schleicher@unibas.ch</a></span></a>
                    | <a moz-do-not-send="true"
                      href="http://www.biozentrum.unibas.ch/" class="">
                      <span style="color:#0563C1" class="">www.biozentrum.unibas.ch</span></a>
                    | <a moz-do-not-send="true"
                      href="http://www.microscopynetwork.unibas.ch/"
                      class="">
                      <span style="color:#0563C1" class="">www.microscopynetwork.unibas.ch</span></a><o:p
                      class=""></o:p></p>
                  <p class="MsoNormal"><o:p class=""> </o:p></p>
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      <br>
      <span style="font-size:10pt;">The University of Dundee is a
        registered Scottish Charity, No: SC015096</span>
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      <pre wrap="">_______________________________________________
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</pre>
    </blockquote>
    <br>
    <pre class="moz-signature" cols="72">-- 
Damir Sudar - Staff Scientist
Lawrence Berkeley Natl Laboratory / MBIB
One Cyclotron Road, MS 977, Berkeley, CA 94720, USA
T: 510/486-5346 - F: 510/486-5586 - E: <a class="moz-txt-link-abbreviated" href="mailto:DSudar@lbl.gov">DSudar@lbl.gov</a>

Visiting Scientist, Oregon Health & Science University</pre>
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