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Dear Bio-Formats experts,
<div><br>
I attached a TIF file which does not open correctly using the
latest version of Bio-Format. Note that older versions of
Bio-Format loaded the file differently (data were different) but
weren't correct either. Here is the original message from the user
who generated the image :<br>
<br>
<<<br>
</div>
<div>I use <a
href="http://www.imnc.in2p3.fr/pagesperso/deroulers/software/ndpitools/"
target="_blank" rel="nofollow"
onmousedown="this.href='http://www.google.com/url?q\75http%3A%2F%2Fwww.imnc.in2p3.fr%2Fpagesperso%2Fderoulers%2Fsoftware%2Fndpitools%2F\46sa\75D\46sntz\0751\46usg\75AFQjCNFzdq1e6Lsn-N4R1UCrXYfBvHQahA';return
true;"
onclick="this.href='http://www.google.com/url?q\75http%3A%2F%2Fwww.imnc.in2p3.fr%2Fpagesperso%2Fderoulers%2Fsoftware%2Fndpitools%2F\46sa\75D\46sntz\0751\46usg\75AFQjCNFzdq1e6Lsn-N4R1UCrXYfBvHQahA';return
true;">ndpisplit</a> under Linux to split an NDPI file. However,
Icy (latest version) doesn't read these files correctly and shows
weird colors. My first guess is that the files were not RGB, but
the tool "identify" by ImageMagic says indeed "Colorspace: sRGB".</div>
<div>I converted the image to YCrCb in MATLAB and I think it looks
like the one showed by Icy.</div>
<div>Icy shows it correctly when I open the ndpi file directly (if
the image is not huge), but sometimes I'll prefer to open the
split image.</div>
<div>I attach an image that Icy can't read correctly.</div>
>><br>
<br>
Note that you can replace Icy by Fiji/ImageJ, i tested on both
(using Bio-Formats importer) and the problem is the same.<br>
<br>
Best,<br>
<br>
<pre class="moz-signature" cols="72">--
Stephane Dallongeville
Unité d'Analyse d'Images Biologiques
CNRS UMR 3691
Institut Pasteur
25 rue du Dr. Roux
75724 Paris cedex 15 (FRANCE)
Tel: +33 (0)1 45 68 87 01
Fax: +33 (0)1 40 61 33 30
<a class="moz-txt-link-freetext" href="http://www.bioimageanalysis.org/">http://www.bioimageanalysis.org/</a></pre>
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