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Hi Michael,
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</div>
<div class="">thanks for your feedback.</div>
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<div class="">Reading through your email, I suppose you are opening your nd2 files</div>
<div class="">using ImageJ. Which version of Bio-Formats did you download and use</div>
<div class="">for opening the files?</div>
<div class="">Also when the file opening stops, do you receive a warning or an</div>
<div class="">error message or does the application exit?</div>
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</div>
<div class="">Assuming the dataset started to load correctly, there might be a memory</div>
<div class="">issue. This page of the Bio-Formats user documentation [1] provides</div>
<div class="">some suggestions for dealing with large datasets. Is the dataset loaded</div>
<div class="">using the virtual stack for instance?</div>
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</div>
<div class="">Best regards,</div>
<div class="">Sebastien</div>
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</div>
<div class="">[1] <a href="https://www.openmicroscopy.org/site/support/bio-formats5.1/users/imagej/managing-memory.html" class="">https://www.openmicroscopy.org/site/support/bio-formats5.1/users/imagej/managing-memory.html</a></div>
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<blockquote type="cite" class="">
<div class="">On 22 Sep 2015, at 17:39, Cammer, Michael <<a href="mailto:Michael.Cammer@med.nyu.edu" class="">Michael.Cammer@med.nyu.edu</a>> wrote:</div>
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<p class="MsoNormal">We are having a problem opening .nd2 files.<o:p class=""></o:p></p>
<p class="MsoNormal">We have this problem is an older version of LOCI and a daily build we downloaded late last week.<o:p class=""></o:p></p>
<p class="MsoNormal"><o:p class=""> </o:p></p>
<p class="MsoNormal">Each file is 17.8 GB with 55 timepoints of 2 channels of 15 Z positions.<o:p class=""></o:p></p>
<p class="MsoNormal"><o:p class=""> </o:p></p>
<p class="MsoNormal">LOCI opens the first 27 timepoints, 2 channels, and 7 Z of the 28<sup class="">th</sup> timepoint and then just stops.
<o:p class=""></o:p></p>
<p class="MsoNormal"><o:p class=""> </o:p></p>
<p class="MsoNormal">If we export the file from Nikon Elements to OME TIFF, the whole thing can be operated on fine, but it would be preferable to be able to open directly into ImageJ.<o:p class=""></o:p></p>
<p class="MsoNormal"><o:p class=""> </o:p></p>
<p class="MsoNormal">Thank you.<o:p class=""></o:p></p>
<p class="MsoNormal"><o:p class=""> </o:p></p>
<p class="MsoNormal"><span style="font-size:10.0pt" class="">=========================================================================<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt" class="">Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt" class=""> Cell: 914-309-3270 **
</span><b class=""><span style="font-size:10.0pt;font-family:"Adobe Fan Heiti Std B","sans-serif"" class="">MY OFFICE HAS MOVED TO SKIRBALL 2<sup class="">nd</sup> FLOOR, Back right **</span></b><i class=""><span style="font-size:10.0pt" class=""><o:p class=""></o:p></span></i></p>
<p class="MsoNormal"><span style="font-size:10.0pt" class=""> <a href="http://ocs.med.nyu.edu/microscopy" class="">
<span style="color:blue" class="">http://ocs.med.nyu.edu/microscopy</span></a> & <a href="http://microscopynotes.com/" class="">
<span style="color:blue" class="">http://microscopynotes.com/</span></a><o:p class=""></o:p></span></p>
<p class="MsoNormal"><o:p class=""> </o:p></p>
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