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<div class="">Dear Sho,</div>
<div class=""><br class="">
</div>
<div class="">thanks for reporting this issue. From your description it looks like this is a bug</div>
<div class="">in Bio-Formats. Can you confirm the Bio-Formats version you are using is 5.1.2</div>
<div class="">from the '<em class="menuselection">Help > About Plugins > Bio-Formats Plugins...</em>.' menu in Fiji?</div>
<div class=""><br class="">
</div>
<div class="">Uploading sample files would be ideal for us to assess the nature of the error.</div>
<div class="">If your files are smaller than 2GB, you can try to upload them at </div>
<div class=""><a href="https://www.openmicroscopy.org/qa2/qa/upload/" class="">https://www.openmicroscopy.org/qa2/qa/upload/</a>. If this is too big, uploading</div>
<div class="">the .nd file and the list of the TIFF file names would definitely be a good start.</div>
<div class="">Alternatively, we can provide the details of an FTP server to upload larger</div>
<div class="">datasets. Let us know which solution is the most adapted to you.</div>
<div class=""><br class="">
</div>
<div class="">Best regards,</div>
<div class="">Sebastien</div>
<br class="">
<div>
<blockquote type="cite" class="">
<div class="">On 15 Jun 2015, at 20:37, <a href="mailto:fujisaws@mskcc.org" class="">
fujisaws@mskcc.org</a> wrote:</div>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">There might be a bug in opening .nd files in FIJI.<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class=""> </span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">My user took a multi-channel, multi-position z-stack timelapse using a widefield system controlled by Metamorph software. You get an ".nd" file that compiles
all the individual tif files.<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class=""> </span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">When I open the .nd file in FIJI, one of 3 channels acquired is always from the wrong xy position, except for the first position. The images, obviously open
fine in Metamorph.<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class=""> </span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">Has anybody seen something like this and know a work around? I will be happy to upload the files, but they are rather big.<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class=""> </span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class=""> </span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">Sho Fujisawa<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">Sr. Digital Microscopist<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">Molecular Cytology Core Facility<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">Memorial Sloan-Kettering Cancer Center<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">417 E. 68th St.
<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">Zuckerman Room 1834<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">New York NY 10065<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">(212) 639-6667<o:p class=""></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"" class="">(646) 888-2186<o:p class=""></o:p></span></p>
<p class="MsoNormal"><o:p class=""> </o:p></p>
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