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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">There might be a bug in opening .nd files in FIJI.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">My user took a multi-channel, multi-position z-stack timelapse using a widefield system controlled by Metamorph software. You get an ".nd" file that compiles all the
individual tif files.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">When I open the .nd file in FIJI, one of 3 channels acquired is always from the wrong xy position, except for the first position. The images, obviously open fine in
Metamorph.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">Has anybody seen something like this and know a work around? I will be happy to upload the files, but they are rather big.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">Sho Fujisawa<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">Sr. Digital Microscopist<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">Molecular Cytology Core Facility<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">Memorial Sloan-Kettering Cancer Center<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">417 E. 68th St.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">Zuckerman Room 1834<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">New York NY 10065<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">(212) 639-6667<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Bookman Old Style","serif"">(646) 888-2186<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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