<div dir="ltr"><div class="gmail_quote">Hi all-</div><div class="gmail_quote"><div dir="ltr"><div><br></div><div>I have cross-posted this issue to both this list and the ImageJ list, as I'm not sure which is the best place to ask the question.</div><div><br></div><div>Similar to recent past posts on this list, I'm having trouble saving files that are > 4GB into ome.tif or IDS/ICS formats. I have a 5GB file, open as a virtual file, and when I go to export via Bioformats Exporter to IDS/ICS format, I get the following error:</div><div><br></div><div><div>(Fiji Is Just) ImageJ 2.0.0-rc-15/1.49p; Java 1.6.0_24 [64-bit]; Windows 7 6.1; 98MB of 12209MB (<1%)</div><div> </div><div>java.lang.IllegalArgumentException: Negative position</div><div><span style="white-space:pre-wrap"> </span>at sun.nio.ch.FileChannelImpl.read(FileChannelImpl.java:600)</div><div><span style="white-space:pre-wrap"> </span>at loci.common.NIOByteBufferProvider.allocateDirect(NIOByteBufferProvider.java:131)</div><div><span style="white-space:pre-wrap"> </span>at loci.common.NIOByteBufferProvider.allocate(NIOByteBufferProvider.java:116)</div><div><span style="white-space:pre-wrap"> </span>at loci.common.NIOFileHandle.buffer(NIOFileHandle.java:551)</div><div><span style="white-space:pre-wrap"> </span>at loci.common.NIOFileHandle.seek(NIOFileHandle.java:273)</div><div><span style="white-space:pre-wrap"> </span>at loci.common.RandomAccessOutputStream.seek(RandomAccessOutputStream.java:79)</div><div><span style="white-space:pre-wrap"> </span>at loci.formats.out.ICSWriter.saveBytes(ICSWriter.java:139)</div><div><span style="white-space:pre-wrap"> </span>at loci.formats.FormatWriter.saveBytes(FormatWriter.java:126)</div><div><span style="white-space:pre-wrap"> </span>at loci.plugins.out.Exporter.run(Exporter.java:629)</div><div><span style="white-space:pre-wrap"> </span>at loci.plugins.LociExporter.run(LociExporter.java:77)</div><div><span style="white-space:pre-wrap"> </span>at ij.plugin.filter.PlugInFilterRunner.processOneImage(PlugInFilterRunner.java:263)</div><div><span style="white-space:pre-wrap"> </span>at ij.plugin.filter.PlugInFilterRunner.<init>(PlugInFilterRunner.java:112)</div><div><span style="white-space:pre-wrap"> </span>at ij.IJ.runUserPlugIn(IJ.java:201)</div><div><span style="white-space:pre-wrap"> </span>at ij.IJ.runPlugIn(IJ.java:163)</div><div><span style="white-space:pre-wrap"> </span>at ij.Executer.runCommand(Executer.java:131)</div><div><span style="white-space:pre-wrap"> </span>at ij.Executer.run(Executer.java:64)</div><div><span style="white-space:pre-wrap"> </span>at java.lang.Thread.run(Thread.java:662)</div><div><br></div><div><br></div><div>When I go to export to ome.tif format, I get the same error previously reported on this list:</div><div><span style="color:rgb(0,0,0);font-family:Verdana,Geneva,Helvetica,Arial,sans-serif;font-size:13.4399995803833px">loci.formats.FormatException: File is too large; call </span><span style="color:rgb(0,0,0);font-family:Verdana,Geneva,Helvetica,Arial,sans-serif;font-size:13.4399995803833px">setBigTiff(true)</span><br></div><div><span style="color:rgb(0,0,0);font-family:Verdana,Geneva,Helvetica,Arial,sans-serif;font-size:13.4399995803833px"><br></span></div><div><span style="color:rgb(0,0,0);font-family:Verdana,Geneva,Helvetica,Arial,sans-serif;font-size:13.4399995803833px">Is it possible to retain the ome.tif format for >4GB? In my case, this is for importing into Huygens for deconvolution. </span></div><div><span style="color:rgb(0,0,0);font-family:Verdana,Geneva,Helvetica,Arial,sans-serif;font-size:13.4399995803833px"><br></span></div><div><span style="color:rgb(0,0,0);font-family:Verdana,Geneva,Helvetica,Arial,sans-serif;font-size:13.4399995803833px">Thanks so very much</span></div><div><span style="color:rgb(0,0,0);font-family:Verdana,Geneva,Helvetica,Arial,sans-serif;font-size:13.4399995803833px">-Jason</span></div>-- <br><div><div dir="ltr"><div><font face="Times New Roman" size="3">
</font><font color="#000000" face="Times New Roman" size="3">
</font><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:Arial,sans-serif;font-size:10pt"><font color="#000000">Jason
Miller, MD, PhD</font></span></p><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:Arial,sans-serif;font-size:10pt"><font color="#000000">University of Michigan Kellogg Eye Center</font></span></p><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:Arial,sans-serif;font-size:10pt"><font color="#000000">--</font></span></p><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:Arial,sans-serif;font-size:10pt"><font color="#000000">Home address:<br></font></span></p><font color="#000000" face="Times New Roman" size="3">
</font><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:Arial,sans-serif;font-size:10pt"><font color="#000000">117 Worden Ave<br></font></span></p><font color="#000000" face="Times New Roman" size="3">
</font><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:Arial,sans-serif;font-size:10pt"><font color="#000000">Ann Arbor, MI 48103<br></font></span></p><font color="#000000" face="Times New Roman" size="3">
</font><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:Arial,sans-serif;font-size:10pt"><font color="#000000">Cell: </font><a value="+14152252134"><font color="#000000">(415)
225-2134</font></a></span></p><font color="#000000" face="Times New Roman" size="3">
</font><p style="margin:0in 0in 0pt"><font color="#000000"><span style="color:rgb(31,73,125);font-family:Arial,sans-serif;font-size:10pt">E-mail:
</span><b><span style="color:rgb(49,132,155);font-family:Arial,sans-serif;font-size:10pt"><a href="mailto:jason.miller@stanfordalumni.org" target="_blank">jason.miller@stanfordalumni.org</a></span></b></font></p><p style="margin:0in 0in 0pt"><br><font color="#000000"><b><span style="color:rgb(49,132,155);font-family:Arial,sans-serif;font-size:10pt"></span></b></font></p><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:Arial,sans-serif;font-size:10pt"><font color="#000000">"When I was 5 years old, my mother always told me that happiness was the key to life. When I went to school, they asked me what I wanted to be when I grew up. I wrote down, 'Happy.' They told me I didn't understand the assignment. I told them they didn't understand life." - John Lennon<br></font></span></p><span href="http://www.quora.com/What-are-some-of-the-best-and-truest-life-quotes#"></span></div><div><div><font face="Times New Roman" size="3"></font></div></div></div></div>
</div></div>
</div><br><br clear="all"><div><br></div>-- <br><div class="gmail_signature"><div dir="ltr"><div><font face="Times New Roman" size="3">
</font><font color="#000000" face="Times New Roman" size="3">
</font><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:"Arial","sans-serif";font-size:10pt"><font color="#000000">Jason
Miller, MD, PhD</font></span></p><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:"Arial","sans-serif";font-size:10pt"><font color="#000000">University of Michigan Kellogg Eye Center</font></span></p><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:"Arial","sans-serif";font-size:10pt"><font color="#000000">--</font></span></p><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:"Arial","sans-serif";font-size:10pt"><font color="#000000">Home address:<br></font></span></p><font color="#000000" face="Times New Roman" size="3">
</font><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:"Arial","sans-serif";font-size:10pt"><font color="#000000">117 Worden Ave<br></font></span></p><font color="#000000" face="Times New Roman" size="3">
</font><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:"Arial","sans-serif";font-size:10pt"><font color="#000000">Ann Arbor, MI 48103<br></font></span></p><font color="#000000" face="Times New Roman" size="3">
</font><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:"Arial","sans-serif";font-size:10pt"><font color="#000000">Cell: </font><a value="+14152252134"><font color="#000000">(415)
225-2134</font></a></span></p><font color="#000000" face="Times New Roman" size="3">
</font><p style="margin:0in 0in 0pt"><font color="#000000"><span style="color:rgb(31,73,125);font-family:"Arial","sans-serif";font-size:10pt">E-mail:
</span><b><span style="color:rgb(49,132,155);font-family:"Arial","sans-serif";font-size:10pt"><a href="mailto:jason.miller@stanfordalumni.org" target="_blank">jason.miller@stanfordalumni.org</a></span></b></font></p><p style="margin:0in 0in 0pt"><br><font color="#000000"><b><span style="color:rgb(49,132,155);font-family:"Arial","sans-serif";font-size:10pt"></span></b></font></p><p style="margin:0in 0in 0pt"><span style="color:rgb(31,73,125);font-family:"Arial","sans-serif";font-size:10pt"><font color="#000000">"When I was 5 years old, my mother always told me that happiness was the key to life. When I went to school, they asked me what I wanted to be when I grew up. I wrote down, 'Happy.' They told me I didn't understand the assignment. I told them they didn't understand life." - John Lennon<br></font></span></p><span href="http://www.quora.com/What-are-some-of-the-best-and-truest-life-quotes#"></span></div><div><div><font face="Times New Roman" size="3"></font></div></div></div></div>
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