<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><div>Hello,</div><div><br></div><div>I’m using bioformats to read datasets acquired with micromanager (one.tif stacks, split into several files per position when one position is larger than 4gb). Typically, I have 5 positions per experiment (and expect to upscale it up to 20-30); so far I was using this code (imagej macro run using fiji --headless) to open a dataset in a virtual stack:</div><br><blockquote type="cite"><div>path = "/path/to/file_Pos0.ome.tif";</div><div><br></div></blockquote><div></div><blockquote type="cite"><div><font color="#0f61c8">import ij.*;</font></div><div><font color="#0f61c8">import loci.plugins.*;</font></div><div><font color="#0f61c8">import loci.plugins.in.ImporterOptions;</font></div><div><font color="#0f61c8">ImporterOptions options = new ImporterOptions();</font></div><div><font color="#0f61c8">options.setId(path);</font></div><div><font color="#0f61c8">options.setVirtual(true);</font></div><div><font color="#0f61c8">ImagePlus[] imps = BF.openImagePlus(options);</font></div><div><font color="#0f61c8">imps[0].show();</font></div></blockquote><div><br></div><div>I works well with 5.1 beta (in 5.0 only the first position is loaded successfully). I would like to know if it’s possible to specify to load one series only (in particular to disable the parsing of the metadata of other series, e.g. using options.setSeriesOn() without luck so far). For large dataset, this would make a huge difference in performance in my case (parsing can easily reach on minute per position).</div><div><br></div><div>Thank you in advance for your help. Best regards,</div><div><br></div><div>Thomas</div><div><div><br></div><div>--</div><div>Thomas Julou | Computational & Systems Biology | Biozentrum – University of Basel | Klingelbergstrasse 50/70 CH-4056 Basel | +41 (0)61 267 16 21</div></div><div><br></div></body></html>