<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"><base href="x-msg://55/"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div><br></div>Hi Mike, <span class="Apple-tab-span" style="white-space:pre"> </span>(I've cc'd the users list in case anyone else has useful advice or would benefit from this).<div><div><br></div><div> You can configure this as described here: <a href="https://www.openmicroscopy.org/site/support/omero5/sysadmins/grid.html#ice-messagesizemax">https://www.openmicroscopy.org/site/support/omero5/sysadmins/grid.html#ice-messagesizemax</a></div><div><br></div><div>However, if you have a very large ROI (over approx 3000 x 3000 pixels) then the current script will fail when it tries to create a new image,</div><div>since "Big" images over this threshold are tiled and cannot be created from a single plane at a time.</div><div><br></div><div>This was discussed at length on our forums (which starts with the same error you have) at</div><div><a href="https://www.openmicroscopy.org/community/viewtopic.php?f=6&t=7565">https://www.openmicroscopy.org/community/viewtopic.php?f=6&t=7565</a></div><div><br></div><div>And concludes with a modified version of the script which you can find at:</div><div><a href="https://github.com/ebbwdan/omero_scripts/blob/master/omero/util_scripts/Slide_Scanner_Images_From_ROIs.py">https://github.com/ebbwdan/omero_scripts/blob/master/omero/util_scripts/Slide_Scanner_Images_From_ROIs.py</a></div><div><br></div><div>So maybe give that script a try - although no guarantees it'll work for your images since it may not have</div><div>been tested as much as the "official" released scripts.</div><div><br></div><div><br></div><div> Hope that helps,</div><div><br></div><div> Will.</div><div><br></div><div><br></div><div><div><br><div><div>On 20 Jan 2015, at 16:49, Michael McFarland <<a href="mailto:Michael.McFarland@jax.org">Michael.McFarland@jax.org</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div lang="EN-US" link="blue" vlink="purple" style="font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div class="WordSection1" style="page: WordSection1; "><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Hello, Will –<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">I got your email address from the Python script. I run the script but can’t<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">find any images afterwards.<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Here is my situation:<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Project: KOMP<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Dataset: ABR<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Image ID: 7532<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">ROI: I have two. One rectangle<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">I have a dataset named From_ROIs<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">I run the script and the log shows:<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span style="font-size: 10pt; font-family: 'Courier New'; ">unknown = Ice::MemoryLimitException<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span style="font-size: 10pt; font-family: 'Courier New'; ">Ice.MemoryLimitException<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span style="font-size: 10pt; font-family: 'Courier New'; "> reason = "requested 139264030 bytes, maximum allowed is 67108864 bytes (see Ice.MessageSizeMax)"<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Is there a way I can configure this?<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Thanks!<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">- Mike<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div></div><p>The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible.</p></div></blockquote></div><br></div></div></div></body></html>