<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi, Moore,<div class=""><br class=""></div><div class="">I think you got my idea.</div><div class="">I mean when you take the photo using the microscopy, you got a nd2 file. The nd2 file should record the actual time each image was taken.</div><div class="">The nd2 file is a video consists of all images. Therefore, each image in the nd2 file should contain the information of the </div><div class="">actual time that the image is taken. when I use bfconvert to convert the nd2 file to tiff file, I want to save that information also.</div><div class=""><br class=""></div><div class="">Using %A may give me the actual time the first image was taken in the nd2 file. However, it could not give the actual time all </div><div class="">the other images was taken in the same nd2 file. Due to there might be something wrong that the image was not taken as the the time I set. So I need to get the information of the actual time the image was taken. </div><div class=""><br class=""></div><div class="">So my question is that the <b class="">actual time</b> the image was taken is still conserved when I use bfconvert to convert nd2 to tiff, and how can I get that information (<b class="">actual time</b>)?</div><div class=""><br class=""></div><div class="">Thank you so much and best regards!</div><div class="">Fangfei</div><div class=""><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On Dec 16, 2014, at 11:19 AM, Melissa Linkert <<a href="mailto:melissa@glencoesoftware.com" class="">melissa@glencoesoftware.com</a>> wrote:</div><br class="Apple-interchange-newline"><div class="">Hi Fangfei,<br class=""><br class=""><blockquote type="cite" class=""><blockquote type="cite" class=""><blockquote type="cite" class="">When I use bfconvert to convert a nd2 file into a number of tiff files,<br class="">is there a way to extract the timestamp of each image, i.e., the time<br class="">each image is taken by microscope camera?<br class=""></blockquote></blockquote></blockquote><br class=""><blockquote type="cite" class="">It seems like Fangfei wanted to know the timestamp of each tiff,<br class="">E.g. timepoint 0 is 0 seconds<br class=""><span class="Apple-tab-span" style="white-space:pre"> </span>timepoint 1 is 10.5 seconds<br class=""></blockquote><br class="">If you are indeed hoping to record the timestamp, the only way to do so<br class="">now is by using the %A option in the output file name, for example:<br class=""><br class=""> bfconvert file.nd2 output-file_%A.tiff<br class=""><br class="">%A gives the full timestamp of the first image stored in the output<br class="">file. So if the start of the acquisition was 2014-12-16T10:15:00 and<br class="">the first image was acquired 5 seconds later, the output file name would<br class="">be output-file_2014-12-16T10:15:05.tiff. There is not currently a<br class="">built-in way of recording just the timestamp from the beginning of the<br class="">acquisition, but it could be added if that would be useful.<br class=""><br class="">Regards,<br class="">-Melissa<br class=""><br class="">On Tue, Dec 16, 2014 at 12:11:23PM +0000, William Moore wrote:<br class=""><blockquote type="cite" class="">It seems like Fangfei wanted to know the timestamp of each tiff,<br class="">E.g. timepoint 0 is 0 seconds<br class=""><span class="Apple-tab-span" style="white-space:pre"> </span>timepoint 1 is 10.5 seconds<br class=""><span class="Apple-tab-span" style="white-space:pre"> </span><span class="Apple-tab-span" style="white-space:pre"> </span>etc.<br class=""><br class="">Am I right?<br class=""><br class="">On 16 Dec 2014, at 11:48, "Balaji Ramalingam (Staff)" <<a href="mailto:b.ramalingam@dundee.ac.uk" class="">b.ramalingam@dundee.ac.uk</a>> wrote:<br class=""><br class=""><blockquote type="cite" class="">Hi,<br class=""><br class="">If the question is about converting each time point into an image,<br class="">The following example should help,<br class=""><br class="">bfconvert -timepoint 0 /path/to/input output-first-timepoint.tiff<br class=""><br class=""><br class="">The above example converts the first time point alone (-timepoint 0).<br class=""><br class="">For more information on bfconvert, please follow the following link,<br class=""><a href="http://www.openmicroscopy.org/site/support/bio-formats5/users/comlinetools/" class="">http://www.openmicroscopy.org/site/support/bio-formats5/users/comlinetools/</a><br class="">conversion.html<br class=""><br class="">Hope that helps.<br class=""><br class="">Best,<br class="">Balaji<br class=""><br class="">__________________<br class="">Mr Balaji Ramalingam<br class="">Software Developer<br class=""><br class="">OME Team<br class="">College of Life Sciences<br class="">University of Dundee<br class=""><br class=""><br class=""><br class=""><br class=""><br class=""><br class="">On 15/12/2014 18:59, "FangfeiLi" <lifangfei@hotmail.com> wrote:<br class=""><br class=""><blockquote type="cite" class="">Hello,<br class=""><br class="">May I ask a question?<br class=""><br class="">When I use bfconvert to convert a nd2 file into a number of tiff files,<br class="">is there a way to extract the timestamp of each image, i.e., the time<br class="">each image is taken by microscope camera?<br class=""><br class="">Thank you very much and best regards!<br class="">Fangfei<br class="">_______________________________________________<br class="">ome-users mailing list<br class="">ome-users@lists.openmicroscopy.org.uk<br class="">http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users<br class=""></blockquote><br class=""><br class="">The University of Dundee is a registered Scottish Charity, No: SC015096<br class="">_______________________________________________<br class="">ome-users mailing list<br class="">ome-users@lists.openmicroscopy.org.uk<br class="">http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users<br class=""></blockquote><br class="">_______________________________________________<br class="">ome-users mailing list<br class=""><a href="mailto:ome-users@lists.openmicroscopy.org.uk" class="">ome-users@lists.openmicroscopy.org.uk</a><br class="">http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users<br class=""></blockquote></div></blockquote></div><br class=""></div></div></body></html>