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<div>Hi,</div>
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<div>Memory management is very important in such cases, and there are multiple ways to handle these files.</div>
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<div>The documentation for the same is given in here,</div>
<div><a href="http://www.openmicroscopy.org/site/support/bio-formats5/users/imagej/managing-memory.html">http://www.openmicroscopy.org/site/support/bio-formats5/users/imagej/managing-memory.html</a></div>
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<div>Please try the suggestions in here and let us know if the issue persists.</div>
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<div>Best,</div>
<div>Balaji</div>
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<div style="color: rgb(40, 40, 40);"><span style="background-color: rgb(255, 255, 255);">__________________</span></div>
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<p style="margin-top: 0px; margin-bottom: 0px;">Mr Balaji Ramalingam</p>
<p style="margin-top: 0px; margin-bottom: 0px;">Software Developer<br>
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<p style="margin-top: 0px; margin-bottom: 0px;">OME Team</p>
<p style="margin-top: 0px; margin-bottom: 0px;">College of Life Sciences</p>
<p style="margin-top: 0px; margin-bottom: 0px;">University of Dundee</p>
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<span style="font-weight:bold">From: </span>Trevor Steve <<a href="mailto:tsteve@ualberta.ca">tsteve@ualberta.ca</a>><br>
<span style="font-weight:bold">Date: </span>Tuesday, 9 September 2014 17:51<br>
<span style="font-weight:bold">To: </span>"<a href="mailto:ome-users@lists.openmicroscopy.org.uk">ome-users@lists.openmicroscopy.org.uk</a>" <<a href="mailto:ome-users@lists.openmicroscopy.org.uk">ome-users@lists.openmicroscopy.org.uk</a>><br>
<span style="font-weight:bold">Subject: </span>[ome-users] Analyzing extremely large images from AxioScan/ZEN software<br>
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<div dir="ltr">Hi All,
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<div>I am attempting to analyze extremely large images produced on an AxioScan microscope and segmented using ZEN software. These are CZI files with JPEG-XR compression. </div>
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<div>Examples of my image sizes (pixels) are:</div>
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<div style="font-family:arial,sans-serif;font-size:12.8000001907349px">201276 X 203751</div>
<div style="font-family:arial,sans-serif;font-size:12.8000001907349px">205353 X 198345</div>
<div style="font-family:arial,sans-serif;font-size:12.8000001907349px">182611 X 215607</div>
<div style="font-family:arial,sans-serif;font-size:12.8000001907349px">167046 X 189615</div>
<div style="font-family:arial,sans-serif;font-size:12.8000001907349px">135139 X 188422</div>
<div style="font-family:arial,sans-serif;font-size:12.8000001907349px">158073 X 165835</div>
<div style="font-family:arial,sans-serif;font-size:12.8000001907349px">185008 X 144463</div>
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<div style="font-family:arial,sans-serif;font-size:12.8000001907349px"><br>
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<div style="font-family:arial,sans-serif;font-size:12.8000001907349px">When I run the .CZI to .TIF converter <a href="http://hcbi.fas.harvard.edu/resources_software" target="_blank" style="font-size:12.8000001907349px">http://hcbi.fas.harvard.edu/resources_software</a></div>
<div style="font-family:arial,sans-serif;font-size:12.8000001907349px">I end up with TIF files which are, as an example:</div>
<div style="font-family:arial,sans-serif;font-size:12.8000001907349px"><span style="font-size:12.8000001907349px">182612 x 215608 (> 39 GPIX), 74.3-80.2 GB</span><br>
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<div style="font-family:arial,sans-serif;font-size:12.8000001907349px"><span style="font-size:12.8000001907349px"><br>
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<div style="font-family:arial,sans-serif;font-size:12.8000001907349px"><span style="font-size:12.8000001907349px">These massive TIFF files cannot be opened by ImageJ (Bio-formats), Fiji, or Photoshop (CS5). </span><br>
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<div style="font-family:arial,sans-serif;font-size:12.8000001907349px"><span style="font-size:12.8000001907349px"><br>
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<div style="font-family:arial,sans-serif;font-size:12.8000001907349px"><span style="font-size:12.8000001907349px">I am wondering if anyone has found a solution to working with this type of data. Is it possible to reduce the resolution/size of the images produced
by the microscope/ZEN software?</span></div>
<div style="font-family:arial,sans-serif;font-size:12.8000001907349px"><span style="font-size:12.8000001907349px"><br>
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<div style="font-family:arial,sans-serif;font-size:12.8000001907349px"><span style="font-size:12.8000001907349px">Thanks, </span></div>
<div style="font-family:arial,sans-serif;font-size:12.8000001907349px"><span style="font-size:12.8000001907349px"><br>
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<div style="font-family:arial,sans-serif;font-size:12.8000001907349px"><span style="font-size:12.8000001907349px">Trevor</span></div>
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-- <br>
<div dir="ltr">Trevor Steve, MD
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<div>Neurology Resident (PGY-7)</div>
<div>PhD Student, Department of Medicine</div>
<div>University of Alberta</div>
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<span style="font-size:10pt;">The University of Dundee is a registered Scottish Charity, No: SC015096</span>
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