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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal>Hi, <o:p></o:p></p><p class=MsoNormal>I am trying to split and save each channel of a dual color time-labs OME-TIFF using the Bioformats Exporter within FIJI.<o:p></o:p></p><p class=MsoNormal>When I export as .tif, the channels get split, but the OME time stamp for each frame is lost.<o:p></o:p></p><p class=MsoNormal>When I export as ome.tif, the OME time stamp is maintained but the channels are not split. The exporter creates two files, with same content as the original file. <o:p></o:p></p><p class=MsoNormal>Does anyone have a solution how to split channels and preserve the channel specific OME data?<o:p></o:p></p><p class=MsoNormal>Thanks for your support<o:p></o:p></p><p class=MsoNormal>Best Titus<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>(FIJI MACRO)<o:p></o:p></p><p class=MsoNormal>input = getDirectory("Choose Source Directory ");<o:p></o:p></p><p class=MsoNormal>output = input;<o:p></o:p></p><p class=MsoNormal>setBatchMode(true);<o:p></o:p></p><p class=MsoNormal>list = getFileList(input);<o:p></o:p></p><p class=MsoNormal>for (i = 0; i < list.length; i++)<o:p></o:p></p><p class=MsoNormal> processFile(input, output, list[i]);<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>function processFile(input, output, filename)<o:p></o:p></p><p class=MsoNormal style='text-indent:36.0pt'>{<o:p></o:p></p><p class=MsoNormal> a = input + filename;<o:p></o:p></p><p class=MsoNormal> run("Bio-Formats Importer", "open=a color_mode=Composite view=Hyperstack stack_order=XYCZT"); <o:p></o:p></p><p class=MsoNormal> name = getTitle;<o:p></o:p></p><p class=MsoNormal> b = output+name+'.tif'; //ome.tif<o:p></o:p></p><p class=MsoNormal> run("Bio-Formats Exporter", "save=["+b+"] write_each_channel compression=Uncompressed");<o:p></o:p></p><p class=MsoNormal> close();<o:p></o:p></p><p class=MsoNormal> call("java.lang.System.gc");<o:p></o:p></p><p class=MsoNormal style='text-indent:36.0pt'>}<o:p></o:p></p><p class=MsoNormal>call("java.lang.System.gc");<o:p></o:p></p><p class=MsoNormal>setBatchMode(false);<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Max Planck Institute<o:p></o:p></p><p class=MsoNormal>of Molecular Cell Biology<o:p></o:p></p><p class=MsoNormal><span lang=DE>and Genetics <o:p></o:p></span></p><p class=MsoNormal><span lang=DE>Pfotenhauerstr. 108<o:p></o:p></span></p><p class=MsoNormal><span lang=DE>01307 Dresden<o:p></o:p></span></p><p class=MsoNormal><span lang=DE>Phone: +49 351 210 2846<o:p></o:p></span></p><p class=MsoNormal><span lang=DE>Mobil: +49 176 8457 8797<o:p></o:p></span></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>