<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Hi Enno,<div><br></div><div>which version of Bio-Formats are you using? The upcoming 5.0.3 release of Bio-Formats</div><div>will include a large set of bug fixes for the ND2 format.</div><div>Could you upload a sample file to <a href="http://qa.openmicroscopy.org.uk/qa/upload/">http://qa.openmicroscopy.org.uk/qa/upload/</a> for us to check</div><div>this release also addresses your issues?</div><div><br></div><div>Best regards,</div><div>Sebastien</div><div><br><div><div>On 28 Jul 2014, at 12:19, Enno R. Oldewurtel <<a href="mailto:enno.oldewurtel@uni-koeln.de">enno.oldewurtel@uni-koeln.de</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><meta http-equiv="Content-Type" content="text/html; charset=utf-8"><div dir="ltr"><div>Dear all,<br><br></div><div>I just tried to read in ND2 files via matlab (either using r.openBytes directly or bfopen.m). However, when specifying a sub-image with x-origin>=2, the resulting image is skewed along a diagonal with slope depending on the x-origin, i.e. slope=1 for x-origin=2. The y-origin works with all values.<br>
<br>This is the command I use:<br>test=bfopen(f,2,1,400,300);<br><br></div><div>Using bfopen on a tif-file returns a correctly cropped image. Thus this might be a nd2 specific problem.<br><br></div><div>Has anyone experienced the same problem?<br>
<br></div><div>These are images obtained with x-origin=1 and x-origin=2:<span><x-originEquals1.png></span><span><x-originEquals2.png></span><br>
</div><div><br></div><div>Thanks,<br>Enno<br></div><div><br></div><br clear="all"><br>-- <br>Enno R. Oldewurtel<br>AG Prof. B. Maier / Biophysics Group<br>Biozentrum<br>Universitaet zu Koeln<br>Zuelpicher Str. 47b<br>50674 Koeln<br>
<br><a href="mailto:enno.oldewurtel@uni-koeln.de" target="_blank">enno.oldewurtel@uni-koeln.de</a><br><a href="http://www.biophysics.uni-koeln.de/" target="_blank">http://www.biophysics.uni-koeln.de/</a>
</div>
_______________________________________________<br>ome-users mailing list<br><a href="mailto:ome-users@lists.openmicroscopy.org.uk">ome-users@lists.openmicroscopy.org.uk</a><br>http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users<br></blockquote></div><br></div></body></html>