<html><head>
<meta http-equiv="Content-Type" content="text/html; charset=Windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 14px; font-family: Calibri, sans-serif;"><div>Hi Ian,</div><div><br></div><div>It is just a non-FLIM time course with a z-stack at each time point.</div><div><br></div><div>I could take a FLIM z-stack time course if you want?</div><div><br></div><div>Cheers</div><div><br></div><div>Ewan</div><div><br></div><span id="OLK_SRC_BODY_SECTION"><div style="font-family:Calibri; font-size:11pt; text-align:left; color:black; BORDER-BOTTOM: medium none; BORDER-LEFT: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 0in; PADDING-RIGHT: 0in; BORDER-TOP: #b5c4df 1pt solid; BORDER-RIGHT: medium none; PADDING-TOP: 3pt"><span style="font-weight:bold">From: </span> <Munro>, Ian <<a href="mailto:i.munro@imperial.ac.uk">i.munro@imperial.ac.uk</a>><br><span style="font-weight:bold">Date: </span> Wednesday, 30 April 2014 13:20<br><span style="font-weight:bold">To: </span> "<a href="mailto:ome-users@lists.openmicroscopy.org.uk">ome-users@lists.openmicroscopy.org.uk</a>" <<a href="mailto:ome-users@lists.openmicroscopy.org.uk">ome-users@lists.openmicroscopy.org.uk</a>><br><span style="font-weight:bold">Cc: </span> Ewan McGhee <<a href="mailto:e.mcghee@beatson.gla.ac.uk">e.mcghee@beatson.gla.ac.uk</a>><br><span style="font-weight:bold">Subject: </span> Re: [ome-users] LaVision Biotec Imspector .msr file import with version 5 bio-importer<br></div><div><br></div><div><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
Hi Ewan
<div><br></div><div><font color="#050505">Re the file that you uploaded in March: - z-TC-for Loci.msr </font></div><div><font color="#050505">Can I ask you whether this is a FLIM z-stack or non-FLIM ?</font></div><div><font color="#050505">There seems to be no indication in the file of this.</font></div><div><font color="#050505"><br></font></div><div><font color="#050505"><code class="xml color1" style="white-space: pre; border-top-left-radius: 0px !important; border-top-right-radius: 0px !important; border-bottom-right-radius: 0px !important; border-bottom-left-radius: 0px !important; background-image: none !important; border: 0px !important; bottom: auto !important; float: none !important; height: auto !important; left: auto !important; line-height: 1.1em !important; margin: 0px !important; outline: 0px !important; overflow: visible !important; padding: 0px !important; position: static !important; right: auto !important; top: auto !important; vertical-align: baseline !important; width: auto !important; box-sizing: content-box !important; font-family: Consolas, 'Bitstream Vera Sans Mono', 'Courier New', Courier, monospace !important; font-size: 1em !important; min-height: inherit !important;">For
example The InstrumentMode</code><code class="xml plain" style="white-space: pre; border-top-left-radius: 0px !important; border-top-right-radius: 0px !important; border-bottom-right-radius: 0px !important; border-bottom-left-radius: 0px !important; background-image: none !important; border: 0px !important; bottom: auto !important; float: none !important; height: auto !important; left: auto !important; line-height: 1.1em !important; margin: 0px !important; outline: 0px !important; overflow: visible !important; padding: 0px !important; position: static !important; right: auto !important; top: auto !important; vertical-align: baseline !important; width: auto !important; box-sizing: content-box !important; font-family: Consolas, 'Bitstream Vera Sans Mono', 'Courier New', Courier, monospace !important; font-size: 1em !important; min-height: inherit !important;">=</code><code class="xml string" style="white-space: pre; border-top-left-radius: 0px !important; border-top-right-radius: 0px !important; border-bottom-right-radius: 0px !important; border-bottom-left-radius: 0px !important; background-image: none !important; border: 0px !important; bottom: auto !important; float: none !important; height: auto !important; left: auto !important; line-height: 1.1em !important; margin: 0px !important; outline: 0px !important; overflow: visible !important; padding: 0px !important; position: static !important; right: auto !important; top: auto !important; vertical-align: baseline !important; width: auto !important; box-sizing: content-box !important; font-family: Consolas, 'Bitstream Vera Sans Mono', 'Courier New', Courier, monospace !important; font-size: 1em !important; min-height: inherit !important;">"Single-Beam
Ti:Sa”</code></font></div><div><code class="xml string" style="white-space: pre; border-top-left-radius: 0px !important; border-top-right-radius: 0px !important; border-bottom-right-radius: 0px !important; border-bottom-left-radius: 0px !important; background-image: none !important; border: 0px !important; bottom: auto !important; float: none !important; height: auto !important; left: auto !important; line-height: 1.1em !important; margin: 0px !important; outline: 0px !important; overflow: visible !important; padding: 0px !important; position: static !important; right: auto !important; top: auto !important; vertical-align: baseline !important; width: auto !important; box-sizing: content-box !important; font-family: Consolas, 'Bitstream Vera Sans Mono', 'Courier New', Courier, monospace !important; font-size: 1em !important; min-height: inherit !important;"><font color="#050505">&
the units of time are given as S rather than ns.</font></code></div><div><code class="xml string" style="white-space: pre; border-top-left-radius: 0px !important; border-top-right-radius: 0px !important; border-bottom-right-radius: 0px !important; border-bottom-left-radius: 0px !important; background-image: none !important; border: 0px !important; bottom: auto !important; float: none !important; height: auto !important; left: auto !important; line-height: 1.1em !important; margin: 0px !important; outline: 0px !important; overflow: visible !important; padding: 0px !important; position: static !important; right: auto !important; top: auto !important; vertical-align: baseline !important; width: auto !important; box-sizing: content-box !important; font-family: Consolas, 'Bitstream Vera Sans Mono', 'Courier New', Courier, monospace !important; font-size: 1em !important; min-height: inherit !important;"><font color="#050505"><br></font></code></div><div><code class="xml string" style="white-space: pre; border-top-left-radius: 0px !important; border-top-right-radius: 0px !important; border-bottom-right-radius: 0px !important; border-bottom-left-radius: 0px !important; background-image: none !important; border: 0px !important; bottom: auto !important; float: none !important; height: auto !important; left: auto !important; line-height: 1.1em !important; margin: 0px !important; outline: 0px !important; overflow: visible !important; padding: 0px !important; position: static !important; right: auto !important; top: auto !important; vertical-align: baseline !important; width: auto !important; box-sizing: content-box !important; font-family: Consolas, 'Bitstream Vera Sans Mono', 'Courier New', Courier, monospace !important; font-size: 1em !important; min-height: inherit !important; color: blue !important;"><br></code></div><div>Ian</div><div><br></div></div></div></span></body></html>