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<p class="MsoNormal">Hello,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I am want to read the Nikon .nd2 image file using loci_tools.jar for MATLAB. I have successfully imported the image data using loci_tools.jar with the following MATLAB commands:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="color:#4F81BD">imgdata=bfopen(‘fullfilepath\*.nd2’); %command for opening images using BioFormats MATLAB scripts<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#4F81BD"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#4F81BD">img=imgdata{1,1}{1,1}; %First image in the timeseries<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#4F81BD"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#4F81BD">metadata=imgdata{1,2}; %Metadata in the .nd2 file<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#4F81BD"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#4F81BD">omeMeta = imgdata{1, 4}; %OME Metadata<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">The Image data, and the OME metadata look fine. However, the original metadata is missing the time information. For example, when opening Zeiss .lsm files, I see the time information in metadata as, “*TimeStamp*,*TimeStamp*,…”. Where
can I find this time information for each image plane in the .nd2 files?<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks much,<o:p></o:p></p>
<p class="MsoNormal">Lewis Kraft<o:p></o:p></p>
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