<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=us-ascii">
<meta name="Generator" content="Microsoft Word 12 (filtered medium)">
<style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:Tahoma;
panose-1:2 11 6 4 3 5 4 4 2 4;}
@font-face
{font-family:"Book Antiqua";
panose-1:2 4 6 2 5 3 5 3 3 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0cm;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Times New Roman","serif";}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:purple;
text-decoration:underline;}
span.EmailStyle17
{mso-style-type:personal-reply;
font-family:"Book Antiqua","serif";
color:#1F497D;}
.MsoChpDefault
{mso-style-type:export-only;}
@page WordSection1
{size:612.0pt 792.0pt;
margin:72.0pt 72.0pt 72.0pt 72.0pt;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-GB" link="blue" vlink="purple">
<div class="WordSection1">
<p class="MsoNormal"><font size="2" color="#1f497d" face="Book Antiqua"><span style="font-size:11.0pt;font-family:"Book Antiqua","serif";color:#1F497D">I’ve now tried to open an ics file generated by LOCI using the SLIM plugin (Lifetime) and got the same exceptions.
(All with the most up-to-date version of Fiji.)<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Book Antiqua"><span style="font-size:11.0pt;font-family:"Book Antiqua","serif";color:#1F497D">Paul<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Book Antiqua"><span style="font-size:11.0pt;font-family:"Book Antiqua","serif";color:#1F497D"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">____________________<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Dr. Paul Thomas, Director,<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">The Henry Wellcome Laboratory for Cell Imaging,<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">School of Biological Sciences,<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">University of East Anglia,<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Norwich Research Park,<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Norwich,<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">NR4 7TJ,<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">United Kingdom.<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">e-mail: p.thomas@uea.ac.uk<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Tel: +44-1603-592196<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Fax: +44-1603-592250<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Imaging web-site: http://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Personal web-page: http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Book Antiqua"><span style="font-size:11.0pt;font-family:"Book Antiqua","serif";color:#1F497D"><o:p> </o:p></span></font></p>
<div style="border:none;border-left:solid blue 1.5pt;padding:0cm 0cm 0cm 4.0pt">
<div>
<div style="border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0cm 0cm 0cm">
<p class="MsoNormal"><b><font size="2" face="Tahoma"><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif";font-weight:bold">From:</span></font></b><font size="2" face="Tahoma"><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">
ome-users-bounces@lists.openmicroscopy.org.uk [mailto:ome-users-bounces@lists.openmicroscopy.org.uk]
<b><span style="font-weight:bold">On Behalf Of </span></b>Curtis Rueden<br>
<b><span style="font-weight:bold">Sent:</span></b> 19 February 2013 19:39<br>
<b><span style="font-weight:bold">To:</span></b> daniel.martins@oncology.ox.ac.uk<br>
<b><span style="font-weight:bold">Cc:</span></b> OME-users mailing list<br>
<b><span style="font-weight:bold">Subject:</span></b> Re: [ome-users] [Software Feedback] LOCI plugin bug<o:p></o:p></span></font></p>
</div>
</div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><o:p> </o:p></span></font></p>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">Hi Daniel,<o:p></o:p></span></font></p>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><o:p> </o:p></span></font></p>
<div>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">> When you export a time series let's assume with 5 time points, the<o:p></o:p></span></font></p>
</div>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">> first time point is exported correctly, but then the following ones<o:p></o:p></span></font></p>
</div>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">> have an issue.<o:p></o:p></span></font></p>
</div>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><o:p> </o:p></span></font></p>
</div>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">Please update your Fiji to the latest version (Help > Update Fiji) and try again. If the problem still occurs, let us know. In that case though, we may need a test file
or macro; you can upload one using the Help > Upload Sample Image command if needed.<o:p></o:p></span></font></p>
</div>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><o:p> </o:p></span></font></p>
</div>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">Regards,<o:p></o:p></span></font></p>
</div>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">Curtis<o:p></o:p></span></font></p>
</div>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><o:p> </o:p></span></font></p>
</div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><o:p> </o:p></span></font></p>
<div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">On Tue, Feb 12, 2013 at 10:14 AM, <<a href="mailto:daniel.martins@oncology.ox.ac.uk" target="_blank">daniel.martins@oncology.ox.ac.uk</a>> wrote:<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">Dear all, I have been using your LOCI plugin to export some ics/ids time-series stacks using FIJI. I have noticed there is a bug on this feature. When you export a time
series let's assume with 5 time points, the first time point is exported correctly, but then the following ones have an issue. For example time-point 3 instead of being exported as a single frame, is exported as an ics stack with 3 frames, 2 empty frames and
the last one is the actual single frame you would want to be exported to begin with. In summary, each time-point is exported as a stack with a number of frames equal to the time point number. This bug only seems to occur for ics files. If you could help me
and look into this it would be greatly appreciated.<br>
<br>
All the best,<br>
<br>
Daniel Martins<o:p></o:p></span></font></p>
</div>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><o:p> </o:p></span></font></p>
</div>
</div>
</div>
</div>
</div>
</body>
</html>