<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hi, <div><br></div><div><div style="margin: 0px; font-size: 12px; ">I cannot locate detector gain in any of the OME metadata imported from my MetaMorph "MultiDimensional Acquisition" experiment .nd files. None of the various "gain" attributes seem to have any value. In fact, I can get exposure time for each plane and the "channelName" for each channel, very little else. dumpXML seems to show that very few values mapped into the OME metadata. Checking in Metamorph, I can locate the appropriate metadata in "Image Info>Annotations".</div><div style="margin: 0px; font-size: 12px; min-height: 14px; "><br></div><div style="margin: 0px; font-size: 12px; ">Problem is the same if I open the underlying .stk files individually, instead of the .nd file.</div><div style="margin: 0px; font-size: 12px; min-height: 14px; "><br></div><div style="margin: 0px; font-size: 12px; ">The problem was observed using bfopen in MatLab using the latest 4.4.4 BioFormats library. Shame, because I see the latest update addressed some .nd file functionality.</div><div style="margin: 0px; font-size: 12px; min-height: 14px; "><br></div><div style="margin: 0px; font-size: 12px; ">Not sure if this is a general issue with mapping MetaMorph multi-dimensional acquisition data into the OME metadata scheme. Could also be a problem of the replace-user-and-press-any-key variety, since I am newish at trying to peruse the OME metadata structure.</div><div style="margin: 0px; font-size: 12px; min-height: 14px; "><br></div></div><div style="margin: 0px; font-size: 12px; min-height: 14px; "><a href="https://www.dropbox.com/s/m4bq8l59tq8wkcd/Deficient%20MetaMorph%20md.zip">DropBox link to zipped example data</a></div><div style="margin: 0px; font-size: 12px; min-height: 14px; "><br></div><div style="margin: 0px; font-size: 12px; min-height: 14px; ">Ben Timney</div><div style="margin: 0px; font-size: 12px; min-height: 14px; "><br></div><div>
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div>________________</div><div>Ben Timney, PhD</div><div>Research Associate</div><div>Rout Laboratory,</div><div>1230 York Ave, #213</div><div>New York, NY 10065</div></div></span></span>
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