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<p class="MsoNormal">Hi all,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I’m new to Omero so forgive me if I’m bringing up a “trivial” topic.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I am able to successfully a series of .flex files that were taken using the “automated” acquisition mode on the Opera Microscope.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">However, I also have .flex files that do not come with a .mea or .res file and are around 1 GB in size each.<o:p></o:p></p>
<p class="MsoNormal">These were taken in the so called “manual” mode.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">When I use Omero Insight to import those .flex files, I get the following error:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">2011-07-06 13:42:46,054 205839 [2-thread-1] DEBUG ome.formats.OMEROMetadataStoreClient - omero.model.DetectorSettings:74:0 == Detector:0:0<o:p></o:p></p>
<p class="MsoNormal">2011-07-06 13:42:46,057 205842 [2-thread-1] DEBUG ome.formats.OMEROMetadataStoreClient - containerCache contains 1826 entries.<o:p></o:p></p>
<p class="MsoNormal">2011-07-06 13:42:46,059 205844 [2-thread-1] DEBUG ome.formats.OMEROMetadataStoreClient - referenceCache contains 1261 entries.<o:p></o:p></p>
<p class="MsoNormal">2011-07-06 13:42:46,657 206442 [2-thread-1] INFO ormats.importer.gui.LoggingImportMonitor - INTERNAL_EXCEPTION: D:\Opera Demo\OperaDB\life cells long term\fast kin\B5_1 GFP RFP Draq5 20times 7planes step1.flex<o:p></o:p></p>
<p class="MsoNormal">java.lang.RuntimeException: omero.ValidationException<o:p></o:p></p>
<p class="MsoNormal"> serverStackTrace = "ome.conditions.ValidationException: not-null property references a null or transient value: ome.model.core.PlaneInfo.theC; nested exception is org.hibernate.PropertyValueException: not-null property references a
null or transient value: ome.model.core.PlaneInfo.theC<o:p></o:p></p>
<p class="MsoNormal"> at org.springframework.orm.hibernate3.SessionFactoryUtils.convertHibernateAccessException(SessionFactoryUtils.java:648)<o:p></o:p></p>
<p class="MsoNormal"> at org.springframework.orm.hibernate3.HibernateAccessor.convertHibernateAccessException(HibernateAccessor.java:412)<o:p></o:p></p>
<p class="MsoNormal"> at org.springframework.orm.hibernate3.HibernateInterceptor.invoke(HibernateInterceptor.java:117)<o:p></o:p></p>
<p class="MsoNormal"> at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:172)<o:p></o:p></p>
<p class="MsoNormal"> at org.springframework.transaction.interceptor.TransactionInterceptor.invoke(TransactionInterceptor.java:108)<o:p></o:p></p>
<p class="MsoNormal"> at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:172)<o:p></o:p></p>
<p class="MsoNormal"> at ome.tools.hibernate.ProxyCleanupFilter$Interceptor.invoke(ProxyCleanupFilter.java:231)<o:p></o:p></p>
<p class="MsoNormal"> at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:172)<o:p></o:p></p>
<p class="MsoNormal"> at ome.services.util.ServiceHandler.invoke(ServiceHandler.java:116)<o:p></o:p></p>
<p class="MsoNormal"> at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:172)<o:p></o:p></p>
<p class="MsoNormal"> at org.springframework.aop.framework.JdkDynamicAopProxy.invoke(JdkDynamicAopProxy.java:202)<o:p></o:p></p>
<p class="MsoNormal"> at $Proxy64.doWork(Unknown Source)<o:p></o:p></p>
<p class="MsoNormal"> at ome.services.util.Executor$Impl.execute(Executor.java:371)<o:p></o:p></p>
<p class="MsoNormal"> at ome.services.throttling.Adapter.run(Adapter.java:51)<o:p></o:p></p>
<p class="MsoNormal"> at ome.services.throttling.InThreadThrottlingStrategy.runnableCall(InThreadThrottlingStrategy.java:89)<o:p></o:p></p>
<p class="MsoNormal"> at ome.services.blitz.impl.AbstractAmdServant.runnableCall(AbstractAmdServant.java:150)<o:p></o:p></p>
<p class="MsoNormal"> at ome.services.blitz.impl.MetadataStoreI.saveToDB_async(MetadataStoreI.java:190)<o:p></o:p></p>
<p class="MsoNormal"> at omero.api._MetadataStoreTie.saveToDB_async(_MetadataStoreTie.java:85)<o:p></o:p></p>
<p class="MsoNormal"> at omero.api._MetadataStoreDisp.___saveToDB(_MetadataStoreDisp.java:218)<o:p></o:p></p>
<p class="MsoNormal"> at omero.api._MetadataStoreDisp.__dispatch(_MetadataStoreDisp.java:366)<o:p></o:p></p>
<p class="MsoNormal"> at IceInternal.Incoming.invoke(Incoming.java:159)<o:p></o:p></p>
<p class="MsoNormal"> at Ice.ConnectionI.invokeAll(ConnectionI.java:2037)<o:p></o:p></p>
<p class="MsoNormal"> at Ice.ConnectionI.message(ConnectionI.java:972)<o:p></o:p></p>
<p class="MsoNormal"> at IceInternal.ThreadPool.run(ThreadPool.java:577)<o:p></o:p></p>
<p class="MsoNormal"> at IceInternal.ThreadPool.access$100(ThreadPool.jva:12)<o:p></o:p></p>
<p class="MsoNormal"> at IceInternal.ThreadPool$EventHandlerThread.run(ThreadPool.java:971)<o:p></o:p></p>
<p class="MsoNormal"><o:p></o:p></p>
<p class="MsoNormal"> and few more errors of the sort.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Does anybody of you have any clue or tip on how to proceed in getting those pictures imported successfully?<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Maybe something went wrong during export ? Is there a big in the Importer?
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thank you for any help!<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">--<o:p></o:p></p>
<p class="MsoNormal">Christophe<o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:9.0pt;color:#7F7F7F">................................................................................<o:p></o:p></span></b></p>
<p class="MsoNormalCxSpMiddle" style="mso-margin-top-alt:3.0pt;margin-right:0in;margin-bottom:3.0pt;margin-left:0in;mso-add-space:auto">
<b><span style="font-size:10.0pt;color:gray">Dipl.-Ing. Christophe TREFOIS</span></b><span style="font-size:9.0pt;color:#7F7F7F"><br>
</span><span style="font-size:9.0pt;color:gray">PhD Student<o:p></o:p></span></p>
<p class="MsoNormalCxSpMiddle" style="mso-margin-top-alt:3.0pt;margin-right:0in;margin-bottom:3.0pt;margin-left:0in;mso-add-space:auto">
<i><span style="font-size:9.0pt;color:gray">Ing. Sys. Com. Dipl. EPF<o:p></o:p></span></i></p>
<p class="MsoNormalCxSpMiddle" style="mso-margin-top-alt:3.0pt;margin-right:0in;margin-bottom:3.0pt;margin-left:0in;mso-add-space:auto">
<span style="font-size:9.0pt;color:gray"><br>
<img width="37" height="38" id="Picture_x0020_1" src="cid:image001.jpg@01CC3BEE.FB432A80" alt="Description: cid:image001.jpg@01CACC35.07191DE0"><o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:3.0pt;margin-right:0in;margin-bottom:6.0pt;margin-left:0in">
<span style="font-size:9.0pt;color:gray">Luxembourg Centre for Systems Biomedicine<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-bottom:3.0pt"><span style="font-size:8.0pt;color:gray">UNIVERSITY OF LUXEMBOURG</span><span style="font-size:10.0pt;color:gray"><br>
</span><span style="font-size:8.0pt;color:gray">CAMPUS LIMPERTSBERG<br>
162a, avenue de la Faïencerie<br>
L-1511 Luxembourg<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:3.0pt;margin-right:0in;margin-bottom:3.0pt;margin-left:0in">
<span style="font-size:8.0pt;color:gray">T +352 46 66 44 6124<br>
F +352 46 66 44 6949<br>
</span><a href="http://www.uni.lu/" title="blocked::http://www.uni.lu/
http://www.uni.lu/"><span style="font-size:8.0pt;color:gray">www.lcsb.lu</span></a><span style="font-size:8.0pt;color:#7F7F7F"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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