<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hello,<div><br></div><div>I have been using the combination of Omero for data storage and ImageJ for image analysis. In the past this has been very successful. I am having trouble using omero 4.2 and loci 4.2 together. I can export an ome.tif file from Omero and open it in ImageJ with LOCI and this works. I then do some things to the images and export the data set as an ome.tif with LOCI. This works well. I can then import the file into Omero 4.2 successfully and the image Looks fine in insight. However, If i re-export this data set as an ome.tif file and import into imageJ using LOCI the images are out of order the channels and Zs are mixed up. Using the Omero ImageJ plugin i can download the data set successfully into imageJ with out the rearrangement of the C and Z channels. I tried this a second time with another data set and just opened it with LOCI in ImageJ and resaved the data as ome.tif and imported into Omero. On this data set the Z was transposed to T but is not mangled. It appears to be a problem with Export from LOCI.</div><div><br></div><div><br></div><div>Thanks for your help,</div><div>All the Best,</div><div>Scott</div><div><br></div><div><br></div><br><br><div><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Menlo; "><font class="Apple-style-span" face="'Lucida Sans Unicode'">Scott Grieshaber Ph.D.</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Menlo; "><font class="Apple-style-span" face="'Lucida Sans Unicode'">Assistant Professor</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Menlo; "><font class="Apple-style-span" face="'Lucida Sans Unicode'">University of Florida</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Menlo; "><font class="Apple-style-span" face="'Lucida Sans Unicode'">Department of Oral Biology</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Menlo; "><font class="Apple-style-span" face="'Lucida Sans Unicode'">School of Dentistry</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Menlo; "><font class="Apple-style-span" face="'Lucida Sans Unicode'">PO BOX 100424 Room D5-33D</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Menlo; "><font class="Apple-style-span" face="'Lucida Sans Unicode'">1600 SW Archer Road</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Menlo; "><font class="Apple-style-span" face="'Lucida Sans Unicode'">Gainesville FL 32610</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Menlo; "><font class="Apple-style-span" face="'Lucida Sans Unicode'">(352) 273-8856</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 11px/normal Menlo; "><font class="Apple-style-span" face="'Lucida Sans Unicode'"><a href="mailto:sgriesha@ufl.edu">sgriesha@ufl.edu</a></font></div></div></span></div></body></html>