<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hi Aaron-<div><br><div><div>On 16 Nov 2009, at 09:09, Ponti, Aaron wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div lang="EN-US" link="blue" vlink="purple" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div class="Section1"><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Dear Will and Jason,<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Thanks for your feedback. I am posting my question to the confocal lists right away.<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Jason, I guess we simply don’t have a community that can make pressure on vendors. I am afraid most buyers do not care much about file formats and the like as long as the optics are good.</span></div></div></div></span></blockquote><div><br></div><div>I am much more optimistic-- in the long term. There is alot of interest from vendors and users-- the customers. If we don't push this, the problem only gets worse. Optics ARE important. So is working with your data.</div><div><br></div><div>Cheers,</div><div><br></div><div>Jason</div><div><br></div><br><blockquote type="cite"><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div lang="EN-US" link="blue" vlink="purple" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div class="Section1"><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">a2<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "> ---------------------------------------------------------------------<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">| Dr. Aaron C. Ponti<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">| Friedrich Miescher Institute for Biomedical Research<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">| Facility for Advanced Microscopy and Imaging<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">| Software development<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">| Maulbeerstrasse 66 CH-4058, Basel<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">| WRO-1066.2.16<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">| Tel: +41 61 696 3513<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">| Fax: +41 61 697 3976<o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">|<span class="Apple-converted-space"> </span><a href="http://www.fmi.ch/faim" style="color: blue; text-decoration: underline; ">http://www.fmi.ch/faim</a><o:p></o:p></span></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "> ----------------------------------------------------------------------<o:p></o:p></span></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div><div style="border-right-style: none; border-bottom-style: none; border-left-style: none; border-width: initial; border-color: initial; border-top-style: solid; border-top-color: rgb(181, 196, 223); border-top-width: 1pt; padding-top: 3pt; padding-right: 0in; padding-bottom: 0in; padding-left: 0in; "><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><b><span style="font-size: 10pt; font-family: Tahoma, sans-serif; ">From:</span></b><span style="font-size: 10pt; font-family: Tahoma, sans-serif; "><span class="Apple-converted-space"> </span>Jason Swedlow [<a href="mailto:jason@lifesci.dundee.ac.uk" style="color: blue; text-decoration: underline; ">mailto:jason@lifesci.dundee.ac.uk</a>]<span class="Apple-converted-space"> </span><br><b>Sent:</b><span class="Apple-converted-space"> </span>Saturday, November 14, 2009 9:40 AM<br><b>To:</b><span class="Apple-converted-space"> </span>Ponti, Aaron<br><b>Cc:</b><span class="Apple-converted-space"> </span><a href="mailto:ome-users@lists.openmicroscopy.org.uk" style="color: blue; text-decoration: underline; ">ome-users@lists.openmicroscopy.org.uk</a><span class="Apple-converted-space"> </span>OME-Users<br><b>Subject:</b><span class="Apple-converted-space"> </span>Re: [ome-users] Stage positions from Zeiss LSM 710, Olympus FluoView 1000, Leica SP/SP5<o:p></o:p></span></div></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Hi Aaron-<o:p></o:p></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Just adding one comment. From our contacts with Carl Zeiss MicroImaging we've been informally told that there were no changes in the LSM 710's file formats that we needed to know about. However, these formats are very complex, and something might have slipped by.<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">As Will mentioned, the confocal list server might be a good place to post this (this was the method we used some time ago).<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">OK, climbing on soapbox:<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">The current situation-- arbitrary file formats, with essentially any metadata in any format, and no definitive community notification when things change-- will only change when customers condition their purchases on this information being provided. In an ideal world (ok, a total pipe dream), our OME-XML and Bio-Formats developers would be notified BEFORE new formats were released and provided specifications and samples. We're a long way from that, but can get there, with the community's help. If this community insists that this information will be provided, we won't be having these problems anymore.<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Stepping down. Phew!<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Have a great weekend.<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Cheers,<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Jason<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">On 13 Nov 2009, at 17:28, Will Moore wrote:<o:p></o:p></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><br><br><o:p></o:p></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Hi Aaron,<o:p></o:p></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">The key question is whether the stage position metadata is actually stored in the file. If it is, then it should be possible for BioFormats to identify the correct bit of metadata and store it in the OME data model. <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">We have been working at improving the amount of metadata from these confocal formats that is used to populate the OME model. This takes time to identify how to read the piece of metadata in the original file, and how to map it into the OME model. However, we have so far been successful for the various bits of metadata we've attempted so far. <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">There are a couple of ways to determine whether the stage position metadata is actually in the file:<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Open the file in the proprietary software (Olympus, Zeiss, Lecia) and see if it is displayed. <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Or, open the file in ImageJ, using the BioFormats plugin and look at the "Original Metadata". <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">I've tried these with a sample of the various formats you mentioned, and I don't see that any of them have absolute stage positions. (some examples below)<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">I'm not even sure that BioFormats is reading the Leica stage positions properly. Haven't looked at this before. <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">However, I don't have any LSM 710 files (LSM 510 only), and it's also possible that newer versions of these microscopes might save this data. <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Some of these files seem to have places in the files for this metadata, and it's also possible that the formats might expand to accommodate stage metadata. <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">You could try asking on the confocal lists <a href="http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy" style="color: blue; text-decoration: underline; ">http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy</a><o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "> Sorry I couldn't be more help,<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "> Will. <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Looking at the "original metadata" of a couple of oib files, read by Bioformats (using the ImageJ plugin). NB. This is all the "available" metadata currently read by BioFormats, only a subset of this is used to populate the OME data model. <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">This is the data available for each plane of the image, shown for the first 2 planes of a Z stack.<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : AS Level<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>9999<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : AbsPositionUnitName<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>mm<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : AbsPositionValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Adjust Outward<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Adjust Retrun<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : AnalogPMTGain<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : AnalogPMTOffset<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>2<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Confocal<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>ON<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : CountingPMTGain<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : CountingPMTOffset<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.600000<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : CountingPMTVoltage<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : DataMax<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>4095.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : DataMin<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : DataName<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1h_after_pdt_C001Z001T001.tif<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : DataType<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>WORD<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ExcitationOutPutLevel<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>5<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : HeightConvertValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.207<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : HeightUnit<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>um<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ImageDepth<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>2<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ImageGroup<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>Normal<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ImageHeight<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1024<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ImageType<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>Intensity<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ImageWidth<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1024<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : LUTFileName<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>LUT1<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : LUTParameter<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>530227280<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : LightControl<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>9999<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Magnification<span class="apple-tab-span"><span class="Apple-converted-space"> </span></span>60.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Name<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>COFAFrameImage<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Number<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ObjectiveLens NAValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.35<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ObjectiveLens Name<span class="apple-tab-span"><span class="Apple-converted-space"> </span></span>UPLSAPO 60X O NA:1.35<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ObjectiveLens WDValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>9999.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Observation Mode<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>LSM<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : PMTDetectingMode<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>Analog<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : PMTVoltage<span class="apple-tab-span"><span class="Apple-converted-space"> </span></span>830<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Pan Scale<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>2<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Path<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>.\<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : PinholeDiameter<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>115000<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : PinholeScale<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : PixConvertValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : PixUnit<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>Intensity<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ROIFileName<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>530223744_9038484<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Resolution<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>10.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : RotationValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : RotationValue After Clip<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : RotationValue Before Clip<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>10<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : SamplingClock<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>500000<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ScanSpeed<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>2.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Step<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ValidBitCounts<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>12<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Version<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.0.0.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : WidthConvertValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.207<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : WidthUnit<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>um<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : X Pinhole<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>318<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : XPanValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : Y Pinhole<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>-570<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : YPanValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 0 : ZoomValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : AS Level<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>9999<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : AbsPositionUnitName<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>mm<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : AbsPositionValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Adjust Outward<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Adjust Retrun<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : AnalogPMTGain<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : AnalogPMTOffset<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>3<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Confocal<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>ON<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : CountingPMTGain<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : CountingPMTOffset<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.600000<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : CountingPMTVoltage<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : DataMax<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>4095.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : DataMin<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : DataName<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1h_after_pdt_C002Z001T001.tif<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : DataType<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>WORD<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ExcitationOutPutLevel<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>4<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : HeightConvertValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.207<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : HeightUnit<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>um<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ImageDepth<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>2<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ImageGroup<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>Normal<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ImageHeight<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1024<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ImageType<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>Intensity<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ImageWidth<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1024<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : LUTFileName<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>LUT2<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : LUTParameter<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>530238208<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : LightControl<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>9999<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Magnification<span class="apple-tab-span"><span class="Apple-converted-space"> </span></span>60.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Name<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>COFAFrameImage<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Number<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ObjectiveLens NAValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.35<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ObjectiveLens Name<span class="apple-tab-span"><span class="Apple-converted-space"> </span></span>UPLSAPO 60X O NA:1.35<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ObjectiveLens WDValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>9999.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Observation Mode<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>LSM<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : PMTDetectingMode<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>Analog<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : PMTVoltage<span class="apple-tab-span"><span class="Apple-converted-space"> </span></span>700<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Pan Scale<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>2<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Path<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>.\<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : PinholeDiameter<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>115000<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : PinholeScale<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : PixConvertValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : PixUnit<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>Intensity<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ROIFileName<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>530223744_9038484<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Resolution<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>10.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : RotationValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : RotationValue After Clip<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : RotationValue Before Clip<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>10<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : SamplingClock<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>500000<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ScanSpeed<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>2.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Step<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ValidBitCounts<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>12<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Version<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.0.0.0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : WidthConvertValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0.207<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : WidthUnit<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>um<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : X Pinhole<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>318<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : XPanValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : Y Pinhole<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>-570<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : YPanValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>0<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Image 1 : ZoomValue<span class="apple-tab-span"> <span class="Apple-converted-space"> </span></span>1.0<o:p></o:p></div></div></div></div><div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">It looks like there might be some parameters for storing stage position (e.g. YPanValue) but they are not populated. <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">I also don't see any stage position info displayed in the Olympus software for this file, so I'm assuming that it is not stored. <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">However, I don't have a file format specification to hand, and it's also possible that newer microscopes might store this data. <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Looking at a couple of Leica LIF files, I see that the metadata displayed by the software does not include stage position, but if I look in the exported metadata, I can see StagePosX="0" StagePosY="0" StagePosZ="0". So, the parameters exist in the file but it is not written in this case. This may be because the microscope that acquired this image was not fitted with a motorized stage? <o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">On 13 Nov 2009, at 16:05, Ponti, Aaron wrote:<o:p></o:p></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><br><br><o:p></o:p></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Hello<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">We are investigating the purchase of one of the following confocals: Zeiss LSM 710, Olympus FluoView 1000, and Leica SP/SP5. One of the criteria for the choice is the possibility to read stage positions from the files (using the loci/ome-xml tools). With loci_tools 4.1 it seems that reading the stage positions into the OME schema is supported for the Leica, but is not for the other two. Can anybody confirm this? Does anybody know if stage positions arestored at all in .lsm and .oib files?<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">Thanks<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-converted-space"> </span>---------------------------------------------------------------------<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">| Dr. Aaron C. Ponti<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">| Friedrich Miescher Institute for Biomedical Research<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">| Facility for Advanced Microscopy and Imaging<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">| Software development<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">| Maulbeerstrasse 66 CH-4058, Basel<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">| WRO-1066.2.16<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">| Tel: +41 61 696 3513<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">| Fax: +41 61 697 3976<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">|<span class="Apple-converted-space"> </span><a href="http://www.fmi.ch/faim" style="color: blue; text-decoration: underline; ">http://www.fmi.ch/faim</a><o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-converted-space"> </span>----------------------------------------------------------------------<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">_______________________________________________<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">ome-users mailing list<o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><a href="mailto:ome-users@lists.openmicroscopy.org.uk" style="color: blue; text-decoration: underline; ">ome-users@lists.openmicroscopy.org.uk</a><o:p></o:p></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><a href="http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users" style="color: blue; text-decoration: underline; ">http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users</a><o:p></o:p></div></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; ">William Moore<o:p></o:p></span></div></div><div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; ">Division of Gene Regulation and Expression<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; ">College of Life Sciences<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; ">University of Dundee<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; ">Scotland<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; ">DD1 5PH<o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; "><o:p> </o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; ">Tel 01382 386364<o:p></o:p></span></div></div></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; ">_______________________________________________<br>ome-users mailing list<br><a href="mailto:ome-users@lists.openmicroscopy.org.uk" style="color: blue; text-decoration: underline; ">ome-users@lists.openmicroscopy.org.uk</a><br><a href="http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users" style="color: blue; text-decoration: underline; ">http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users</a><o:p></o:p></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div><div><div><p class="MsoNormal" style="margin-top: 0in; margin-right: 0in; margin-bottom: 12pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p> </o:p></span></p></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">**************************</span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; font-family: Arial, sans-serif; color: black; ">Wellcome Trust Centre for Gene Regulation & Expression</span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; font-family: Arial, sans-serif; color: black; ">College of Life Sciences</span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; font-family: Arial, sans-serif; color: black; ">MSI/WTB/JBC Complex</span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">University of Dundee</span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">Dow Street</span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">Dundee DD1 5EH</span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">United Kingdom</span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p> </o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">phone (01382) 385819</span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">Intl phone: 44 1382 385819 </span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">FAX (01382) 388072 </span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">email:<span class="Apple-converted-space"> </span><a href="mailto:jason@lifesci.dundee.ac.uk" style="color: blue; text-decoration: underline; ">jason@lifesci.dundee.ac.uk</a></span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p> </o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">Lab Page:<span class="Apple-converted-space"> </span><a href="http://gre.lifesci.dundee.ac.uk/staff/jason_swedlow.html" style="color: blue; text-decoration: underline; ">http://gre.lifesci.dundee.ac.uk/staff/jason_swedlow.html</a></span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">Open Microscopy Environment:<span class="Apple-converted-space"> </span><a href="http://openmicroscopy.org" style="color: blue; text-decoration: underline; ">http://openmicroscopy.org</a></span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span class="apple-style-span"><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">**************************</span></span><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p> </o:p></span></div></div><div><div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">The University of Dundee is a Scottish Registered Charity, No. SC015096.<o:p></o:p></span></div></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><br><br></span><span style="font-size: 13.5pt; font-family: Helvetica, sans-serif; color: black; "><o:p></o:p></span></div></div></div><div style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div></div></div></span></blockquote></div><br><div apple-content-edited="true"> <span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="font-family: Helvetica; "><br class="Apple-interchange-newline"><br class="khtml-block-placeholder"></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">**************************</span></div><div style="font-family: Helvetica; "><font class="Apple-style-span" face="Arial">Wellcome Trust Centre for Gene Regulation & Expression</font></div><div style="font-family: Helvetica; "><font class="Apple-style-span" face="Arial">College of Life Sciences</font></div><div style="font-family: Helvetica; "><font class="Apple-style-span" face="Arial">MSI/WTB/JBC Complex</font></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">University of Dundee</span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Dow Street</span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Dundee DD1 5EH</span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">United Kingdom</span></div><div style="font-family: Helvetica; "><br style="font-family: Helvetica; "></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">phone (01382) 385819</span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Intl phone: 44 1382 385819 </span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">FAX (01382) 388072 </span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">email: <a href="mailto:jason@lifesci.dundee.ac.uk">jason@lifesci.dundee.ac.uk</a></span></div><div style="font-family: Helvetica; "><br style="font-family: Helvetica; "></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Lab Page: <a href="http://gre.lifesci.dundee.ac.uk/staff/jason_swedlow.html">http://gre.lifesci.dundee.ac.uk/staff/jason_swedlow.html</a></span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Open Microscopy Environment: <a href="http://openmicroscopy.org">http://openmicroscopy.org</a></span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">**************************</span></div><div style="font-family: Helvetica; "><br class="webkit-block-placeholder"></div><div style="font-family: Helvetica; "><div>The University of Dundee is a Scottish Registered Charity, No. SC015096.</div></div><br class="Apple-interchange-newline"></span><br class="Apple-interchange-newline"></div></span> </div><br></div></body></html>