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<TITLE>Can I convert 1-bit PNG files to 1-bit OME.TIFF files?</TITLE>
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<P ALIGN=LEFT><SPAN LANG="en-au"><FONT COLOR="#000080" SIZE=2 FACE="Arial">All,</FONT></SPAN><SPAN LANG="en-au"><FONT COLOR="#000080" SIZE=2 FACE="Arial"> </FONT></SPAN></P>
<P ALIGN=LEFT><SPAN LANG="en-au"><FONT COLOR="#000080" SIZE=2 FACE="Arial">best wishes for 2009</FONT></SPAN><SPAN LANG="en-au"></SPAN></P>
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<P ALIGN=LEFT><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">Is</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial"></FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"> <FONT COLOR="#000080" SIZE=2 FACE="Arial">it possible to convert 1-bit PNG files (cell-boundaries as a binay image) to 1-bit OME.Tiff files?</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"></SPAN></P>
<P ALIGN=LEFT><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">If I use the bfconvert command line tool, it ends up as a</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"> <FONT COLOR="#000080" SIZE=2 FACE="Arial">UINT8 image</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">…</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"></SPAN></P>
<P ALIGN=LEFT><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">The (compressed) PNG file is only 4 K, the Tiff becomes 3900 K</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">.</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"></SPAN></P>
<P ALIGN=LEFT><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">I have individual overlay files for the CellBodies, the Nuclei and the Neurites.</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"></SPAN></P>
<P ALIGN=LEFT><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">Ultimately, I want to import the</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">se PNG</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial"> file</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">s</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial"> as</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"> <FONT COLOR="#000080" SIZE=2 FACE="Arial">new</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"> <FONT COLOR="#000080" SIZE=2 FACE="Arial">“</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">Channel</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">s</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">”</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial"></FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"> <FONT COLOR="#000080" SIZE=2 FACE="Arial">of an existing file (with already 2 channels)</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"> <FONT COLOR="#000080" SIZE=2 FACE="Arial">to show it as an</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"> <FONT COLOR="#000080" SIZE=2 FACE="Arial">“</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">overlay</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">”</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial"> in the Insight Viewer.</FONT></SPAN></P>
<P ALIGN=LEFT><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">Are there easier ways to do this ?</FONT></SPAN></P>
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<P ALIGN=LEFT><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">Thanks,</FONT></SPAN></P>
<P ALIGN=LEFT><SPAN LANG="en-us"><FONT COLOR="#000080" SIZE=2 FACE="Arial">Best regads, frans</FONT></SPAN><SPAN LANG="en-au"></SPAN><SPAN LANG="en-us"></SPAN></P>
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